中 文 摘 要
目的：探討單次高強度運動誘發之氧化壓力對規律運動及/或長期吸菸者血液氧化指標及抗氧化酶活性的影響。方法：本研究分為控制組（C組）、運動組（E組）、吸菸組（S組）及吸菸加運動組（ES組）共四組，每組人數各為男性8人，共32人。實驗處理以跑步機實施85%VO2max的強度進行單次高強度運動至衰竭，並於運動前、後採集血液樣本分析血漿乳酸濃度、硫巴比妥酸反應物質 (TBARS) 濃度、氧化態低密度脂蛋白濃度 (oxLDL) 及紅血球溶胞液的過氧化氫酶 (CAT) 活性、麩胱甘肽過氧化酶 (GPx) 活性、超氧離子歧化酶 (SOD) 活性等。所得數據以獨立樣本 t 考驗、單因子變異數分析 (ANOVA) 與雙因子(運動 × 吸菸)共變數分析 (ANCOVA) 檢定。結果：除血漿乳酸濃度各組運動後皆顯著高於運動前 (p < .05) 外，其餘指標運動前後並無顯著差異。運動前各指標的比較如下：ES組GPx活性顯著高於E組 (p < .05)；E組、S組及ES組的SOD活性皆顯著高於C組 (p < .05)，且ES組亦顯著高於E組 (p < .05)；E組CAT活性稍高於C組，ES組亦稍高於S組 (惟未達顯著水準)；吸菸組（ES組及S組）血漿oxLDL顯著高於不吸菸組（C組及E組） (p < .05)，而血漿TBARS則無差異。結論：規律運動能增加紅血球抗氧化酶活性，而長期吸菸可能因加重氧化壓力而有加成效果，同時增加心血管疾病危險因子；且單次高強度運動並不會對吸菸者造成更高的氧化壓力。

關鍵詞：長期吸菸、規律運動、抗氧化酶

Abstract
Purpose: To investigate the effects of oxidative stress induced by one bout of high-intensity exercise on blood oxidative indicators and activities of antioxidative enzymes in subjects with regular exercise and/or with chronic cigarette smoking. Methods: There were four groups (8 subjects for each group) : control group (C group), exercise group (E group), smoking group (S group), and exercise-smoking group (ES group). Subjects performed one bout of high-intensity treadmill exercise ( 85％VO2max ) to volitional exhaustion. Blood samples were collected before and immediately after exercise to analyze plasma lactate, thiobarbituric acid reactive substances (TBARS), oxidized low density lipoprotein (oxLDL), and the activity of antioxidative enzymes in erythrocyte lysate, such as catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx). Paired t test, independent one way ANOVA and two-way (exercise × smoking) ANCOVA were used for statistical analysis. Results: Except for plasma lactate, which was significantly higher before exercise than after exercise (p < .05). There were no significant differences between time trials in all other indicators. The comparisons between groups were described as follows: GPx activity was significantly higher in ES group than in E group (p < .05), SOD activity was significantly higher in E group, S group and ES group in comparison with C group (p < .05), and was also significantly higher in ES group than in E group (p < .05). CAT activity was slightly higher in E group than in C group, and also in ES group than in S group, but not statistically. Plasma oxLDL in subjects with cigarette smoking (ES group and S group) was significantly higher than nonsmokers (C group and E group) (p < .05). However, plasma TBARS showed no significant differences between groups. Conclusion: These results indicated that regular exercise can increase the activity of antioxidative enzymes. However, chronic cigarette smoking may have an additive effect, aggravated with oxidative stress; and increase the risks of cardiovascular disease. In addition, one bout of high-intensity may not increase oxidative stress on smokers.

Keywords: chronic cigarette smoking, regular exercise, antioxidative enzymes

一、中文部份
林勁宏（2003）。運動與脂質氧化傷害。中華體育，14(3)，78-86。

行政院衛生署（2004）。統計資料。2005年11月27日，取自行政院衛生署，衛生統計資訊網。
網址http://www.doh.gov.tw/statistic/index.htm

吳其芸（2001）。運動對於人體血液中脂質過氧化物及抗氧化物含量之影響。（碩士論文，台北醫學院）。全國博碩士論文資訊網， 089TMC00058004。

吳家慶(2005)。不同強度動態恢復對損傷肌肉之能及跑步經濟性的影響。未出版博士論文，國立台灣師範大學，台北市。

倪耀華（1992）。運動強度對血漿脂質過氧化物和超氧化物歧化酶活性的影響。中國運動醫學雜誌，11(3)，118。

趙克然, 楊毅軍, 曹道俊（2003）。氧自由基與臨床。台北市：合記。

二、西文部分
Alessio, H. M., & Goldfarb, A. H. (1988). Lipid peroxidation and scavenger enzymes during exercise: adaptive response to training. Journal of Applied Physiology, 64(4), 1333-1336.

Alessio, H. M., Hagerman, A. E., Fulkerson, B. K., Ambrose, J., Rice, R. E., & Wiley, R. L. (2000). Generation of reactive oxygen species after exhaustive aerobic and isometric exercise. Medicine & Science in Sports & Exercise, 32(9), 1576-1581.

Anuradha, C. V., & Balakrishnan, S. D. (1998). Effect of training on lipid peroxidation, thiol status and antioxidant enzymes in tissues of rats. Indian Journal of Physiology and Pharmacology, 42(1), 64-70.

Ashton, T., Rowlands, C. C., Jones, E., Young, I. S., Jackson, S. K., Davies, B., et al. (1998). Electron spin resonance spectroscopic detection of oxygen-centred radicals in human serum following exhaustive exercise. European Journal of Applied Physiology andOccupational Physiology, 77(6), 498-502.

Ayaori, M., Hisada, T., Suzukawa, M., Yoshida, H., Nishiwaki, M., Ito, T., et al. (2000). Plasma levels and redox status of ascorbic acid and levels of lipid peroxidation products in active and passive smokers. Environmental Health Perspectives, 108(2), 105-108.

Balog, T., Sobocanec, S., Sverko, V., Krolo, I., Rocic, B., Marotti, M., et al. (2006). The influence of season on oxidant-antioxidant status in trained and sedentary subjects. Life Sciences, 78(13), 1441-1447.

Brites, F. D., Evelson, P. A., Christiansen, M. G., Nicol, M. F., Basilico, M. J., Wikinski, R. W., et al. (1999). Soccer players under regular training show oxidative stress but an improved plasma antioxidant status. Clinical Science (Lond), 96(4), 381-385.

Chang, C. K., Huang, H. Y., Tseng, H. F., Hsuuw, Y. D., & Tso, T. K. (2006). Interaction of vitamin E and exercise training on oxidative stress and antioxidant enzyme activities in rat skeletal muscles. The Journal of Nutritional Biochemistry.

Church, D. F., & Pryor, W. A. (1985). Free-radical chemistry of cigarette smoke and its toxicological implications. Environmental Health Perspectives, 64, 111-126.

Cooper, C. E., Vollaard, N. B., Choueiri, T., & Wilson, M. T. (2002). Exercise, free radicals and oxidative stress. Biochemical Society Transactions, 30(2), 280-285.

Davies, K. J., Quintanilha, A. T., Brooks, G. A., & Packer, L. (1982). Free radicals and tissue damage produced by exercise. Biochemical and Biophysical Research Communications, 107(4), 1198-1205.

Durak, I., Yalcin, S., Burak Cimen, M. Y., Buyukkocak, S., Kacmaz, M., & Ozturk, H. S. (1999). Effects of smoking on plasma and erythrocyte antioxidant defense systems. Journal of Toxicology and Environmental Health, 56(6), 373-378.

Halliwell, B., & Gutteridge, J. M. (1986). Oxygen free radicals and iron in relation to biology and medicine: some problems and concepts. Archives of Biochemistry and Biophysics, 246(2), 501-514.

Jackson, M. J., Papa, S., Bolanos, J., Bruckdorfer, R., Carlsen, H., Elliott, R. M., et al. (2002). Antioxidants, reactive oxygen and nitrogen species, gene induction and mitochondrial function. Molecular Aspects of Medicine, 23(1-3), 209-285.

Jenkins, R. R. (1988). Free radical chemistry. Relationship to Exercise. Sport of Medicine, 5(3), 156-170.

Kevin, L. G., Novalija, E., & Stowe, D. F. (2005). Reactive oxygen species as mediators of cardiac injury and protection: the relevance to anesthesia practice. Anesthesia and Analgesia, 101(5), 1275-1287.

Kim, S. H., Kim, J. S., Shin, H. S., & Keen, C. L. (2003). Influence of smoking on markers of oxidative stress and serum mineral concentrations in teenage girls in Korea. Nutrition, 19(3), 240-243.

Kim, S. H., Ensunsa, J. L., Zhu, Q. Y., Kim, J. S., Shin, H. S., & Keen, C. L. (2004). An 18-month follow-up study on the influence of smoking on blood antioxidant status of teenage girls in comparison with adult male smokers in Korea. Nutrition, 20(5), 437-444.

Kumar, C. T., Reddy, V. K., Prasad, M., Thyagaraju, K., & Reddanna, P. (1992). Dietary supplementation of vitamin E protects heart tissue from exercise-induced oxidant stress. Molecular and Cellular Biochemistry, 111(1-2), 109-115.

Lee, J., Goldfarb, A. H., Rescino, M. H., Hegde, S., Patrick, S., & Apperson, K. (2002). Eccentric exercise effect on blood oxidative-stress markers and delayed onset of muscle soreness. Medicine & Science in Sports & Exercise, 34(3), 443-448.

Liu, C. S., Lii, C. K., Ou, C. C., Tsai, C. H., Wei, Y. H., & Chen, H. W. (2000). Autoantibody against oxidized low-density lipoproteins may be enhanced by cigarette smoking. Chemico-biological
Interactions, 127(2), 125-137.

Lotito, S. B., & Fraga, C. G. (2000). Catechins delay lipid oxidation and alpha-tocopherol and beta-carotene depletion following ascorbate depletion in human plasma. Proceedings of the Society for Experimental Biology and Medicine, 225(1), 32-38.

Ma, J., Hampl, J. S., & Betts, N. M. (2000). Antioxidant intakes and smoking status: data from the continuing survey of food intakes by individuals 1994-1996. The American Journal of Clinical Nutrition, 71(3), 774-780.

Maehira, F., Zaha, F., Miyagi, I., Tanahara, A., & Noho, A. (2000). Effects of passive smoking on the regulation of rat aortic cholesteryl ester hydrolases by signal transduction. Lipids, 35(5), 503-511.

Mezzetti, A., Lapenna, D., Pierdomenico, S. D., Calafiore, A. M., Costantini, F., Riario-Sforza, G., et al. (1995). Vitamins E, C and lipid peroxidation in plasma and arterial tissue of smokers and non-smokers. Atherosclerosis, 112(1), 91-99.

Miyazaki, H., Oh-ishi, S., Ookawara, T., Kizaki, T., Toshinai, K., Ha, S., 等 (2001). Strenuous endurance training in humans reduces oxidative stress following exhausting exercise. European Journal of Applied Physiology, 84(1-2), 1-6.

Ortenblad, N., Madsen, K., & Djurhuus, M. S. (1997). Antioxidant status and lipid peroxidation after short-term maximal exercise in trained and untrained humans. American Journal of Physiology, 272(4 Pt 2), R1258-1263.

Radak, Z., Kaneko, T., Tahara, S., Nakamoto, H., Ohno, H., Sasvari, M., et al. (1999). The effect of exercise training on oxidative damage of lipids, proteins, and DNA in rat skeletal muscle: evidence for beneficial outcomes. Free Radical Biology and Medicine, 27(1-2), 69-74.

Rissanen, T. H., Voutilainen, S., Nyyssonen, K., Salonen, R., Kaplan, G. A., & Salonen, J. T. (2003). Serum lycopene concentrations and carotid atherosclerosis: the Kuopio Ischaemic Heart Disease Risk Factor Study. The American Journal of Clinical Nutrition, 77(1), 133-138.

Sandhir, R., Subramanian, S., & Koul, A. (2003). Long-term smoking and ethanol exposure accentuates oxidative stress in hearts of mice. Cardiovascular Toxicology, 3(2), 135-140.

Selamoglu, S., Turgay, F., Kayatekin, B. M., Gonenc, S., & Yslegen, C. (2000). Aerobic and anaerobic training effects on the antioxidant enzymes of the blood. Acta Physiologica Hungarica, 87(3), 267-273.

Semin, I., Kayatekin, B. M., Gonenc, S., Acikgoz, O., Uysal, N., Delen, Y., et al. (2000). Lipid peroxidation and antioxidant enzyme levels of intestinal renal and muscle tissues after a 60 minutes exercise in trained mice. Indian Journal of Physiology and Pharmacology, 44(4), 419-427.

Sen, C. K. (2001). Antioxidants in exercise nutrition. Sports Medicine, 31(13), 891-908.

Sen, C. K., & Packer, L. (2000). Thiol homeostasis and supplements in physical exercise. American Journal of Clinical Nutrition, 72(2 Suppl), 653S-669S.

Suzuki, K., Ito, Y., Ochiai, J., Aoki, K., Wakai, K., Tamakoshi, A., et al. (2003). The relationship between smoking habits and serum levels of 8-OHdG, oxidized LDL antibodies, Mn-SOD and carotenoids in rural Japanese residents. Journal of Epidemiology, 13(1), 29-37.

Swain, D. P., & Leutholtz, B. C. (2002). Exercise prescription：a cas study approach to the ACSM guidelines. Champaign, IL: Human Kinetics.

Tsiara, S., Elisaf, M., & Mikhailidis, D. P. (2003). Influence of smoking on predictors of vascular disease. Angiology, 54(5), 507-530.

Van Soeren, M. H., & Graham, T. E. (1998). Effect of caffeine on metabolism, exercise endurance, and catecholamine responses after withdrawal. Journal of Applied Physiology, 85(4), 1493-1501.

Venditti, P., & Di Meo, S. (1997). Effect of training on antioxidant capacity, tissue damage, and endurance of adult male rats. International Journal of Sports Medicine, 18(7), 497-502.

Wei, W., Kim, Y., & Boudreau, N. (2001). Association of smoking with serum and dietary levels of antioxidants in adults: NHANES III, 1988-1994. American Journal of Public Health, 91(2), 258-264.

Yamaguchi, Y., Haginaka, J., Morimoto, S., Fujioka, Y., & Kunitomo, M. (2005). Facilitated nitration and oxidation of LDL in cigarette smokers. European Journal of Clinical Investigation, 35(3), 186-193.

Yamaguchi, Y., Matsuno, S., Kagota, S., Haginaka, J., & Kunitomo, M. (2001). Oxidants in cigarette smoke extract modify low-density lipoprotein in the plasma and facilitate atherogenesis in the aorta of Watanabe heritable hyperlipidemic rabbits. Atherosclerosis, 156(1), 109-117.