目的：探討進行急性低氧運動測試 (4800m test) 前給予抗氧化劑增補對於減緩此測驗造成氧化傷害的影響，同時探討測試完後3天的抗氧化系統改變情形。方法：健康男性大學生12名 (年齡：21.6 ± 0.7 yr，身高：174.9 ± 6.8 cm，體重：78.4 ± 12.6 kg，最大攝氧量：44.4 ± 9.1 ml/kg/min，體脂肪：21.1 ± 3.5 %)以雙盲平衡次序原則分別讓實驗參與者服用麵粉 (control trial)、抗氧化劑(600 mg硫辛酸+1000 mg維生素C+400 IU維生素E)。採血點有：安靜態 (rest)、急性低氧運動測試前 (pre)、急性低氧運動測試後立即 (pt0’)、後30分鐘 (pt30’)、後60分鐘 (pt60’)、後1天 (pt1d)、後3天 (pt3d)。分析紅血球/血漿TBARS、蛋白質氧化傷害指標Carbonyl Protein、紅血球/血漿超氧化離子歧化酶(superoxide dismutase, SOD)、紅血球/血漿過氧化氫酶 (catalase, CAT)、紅血球/血漿麩胱甘肽過氧化酶 (glutathione peroxidase, GPx)、血漿葡萄糖 (glucose)、乳酸 (lactate)、尿酸 (uric acid)。結果：紅血球TBARS不論是對照處理或抗氧化劑處理皆在測試後立即顯著高於安靜態，但抗氧化劑在測試後30分鐘即可顯著降低紅血球TBARS，而無服用抗氧化劑則需至測試後1天才能降低；在測試後3天時間點不論對照處理或抗氧化劑處理皆無差異，但發現對照處理的血漿TBARS顯著高於安靜態，抗氧化劑處理的血漿GPx活性顯著低於安靜態，對照處理則在測試後3天的尿酸、紅血球GPx活性顯著高於安靜態。結論：抗氧化劑增補對於降低急性低氧運動測試所造成的氧化傷害有益，且在測試後3天也無氧化傷害。

Purpose: To investigate the effect of antioxidant supplement on oxidative stress in 4800 m test. Methods: Twelve healthy college students were subjected to two trials: placebo trial and antioxidant trial (600 mg α-lipoic acid, 1000 mg vitamin C, and 400 IU vitamin E). Plasma and RBC levels of thiobarbituric acid reactive substancesm (TBARS), Plasma and RBC glutathione peroxidase activity (GPx), Plasma and RBC catalase activity (CAT), Plasma and RBC superoxide dismutase activity (SOD), lactate, glucose, carbonyl protein, uric acid were measured before treated with supplement (rest), before 4800 m test (pre), after 4800 m test (pt0’), post 30 minute (pt30’), post 60 minute (pt60’), post 1 day (pt1d), post 3 days (pt3d). Results: The 4800 m test significantly increased in the concentration of RBC TBARS for both trials. Antioxidant trial reduced TBARS in pt30’. Control trial had significantly increased in the concentration of plasma TBARS at pt3d. Placebo trial level of uric acid was increased significantly following the 4800 m test. Conclusion: Antioxidant supplement can reduce oxidative stress from 4800 m test within 30 min. Also, it no significantly increased in oxidative stress for 3 days.

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