目的：探討連續服用強效抗氧化劑硫辛酸 (ALA) 對100公里跑步過程中之人體紅血球抗氧化系統與脂質氧化的影響。方法：將13位受試者隨機分為兩組：安慰劑 (CON) 與硫辛酸 (ALA) 組。兩組皆於賽前與每12 km處進行增補與採血，共計增補9次 (1600 mg)、採血10次。比賽全程佩帶心率錶，賽後分析運動成績與運動強度。血漿樣本分析檢測脂質過氧化物(TBARS) 、肌酸激酶 ( CK ) 活性、血糖與乳酸濃度；紅血球樣本分析檢測超氧離子歧化酶 (SOD) 活性、麩胱苷肽過氧化酶 (GPx) 活性與過氧化氫酶(CAT) 活性。所得數據以獨立樣本 t 考驗、混合設計雙因子 (組別 × 距離) 變異數分析 (ANOVA) 檢定。結果：增補硫辛酸對極限耐力跑過程之乳酸、CK與成績表現在組別上沒有達顯著差異。ALA組之血漿TBARS濃度 (3.7 ± 2.0 uM) 顯著低於CON組 (4.9 ± 2.8 uM)( p < .05)。ALA組之血糖濃度 (141.0 ± 14.9 mg‧dL-1) 顯著高於CON組 (130.7 ± 16.1 mg‧dL-1)(p < .05)。ALA組之紅血球SOD活性 (4461.99 ± 677.22 U‧gHb-1) 顯著低於CON組 (5143.49 ± 554.10 U‧gHb-1)( p < .05)。ALA組之紅血球GPx活性 (125.72 ± 22.43 kU‧gHb-1) 顯著高於CON組 (59.32 ±28.20 kU‧gHb-1)(p < .001)。ALA組之紅血球CAT活性 (24.27 ± 11.32 kU‧gHb-1) 顯著低於CON組 (42.56± 10.10 kU‧gHb-1)(p < .001)。結論：於極限耐力跑過程中連續增補硫辛酸能夠降低脂質過氧化傷害，提高紅血球GPx活性。
關鍵詞：硫辛酸、極限耐力跑、氧化傷害

Purpose: To investigate the effects of continuous oral supplementation of alpha-lipoic acid on human antioxidative enzyme activities and lipid peroxidation during extreme endurance running. Methods: A randomized double-blind study was carried out on 13 recreational long-distance runners who were continuously and orally supplemented with placebo (CON, n = 6) or alpha-lipoic acid (LA, n = 7) up to 1600 mg (200 mg for the first 1-7th supplementation and 100 mg for the 8, 9th supplementation) in a 100-km supermarathon race. Heart rates were monitored during running and the plasma concentrations of malondialdehyde (TBARS), glucose, lactate, creatine kinase, as well as antioxidant enzyme activities in red blood cells including superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) were measured 20 min before and at various distances (at 12, 24, 36, 48, 60, 72, 84, 96 and 100 km). Independent t test, mixed design of two-way (group × distance) ANOVA were used for data analysis. Results:. After continuous supplementation of α-lipoic acid in extreme endurance running, plasma lactate, creatine kinase and exercise performance showed no significant differences between groups. TBARS in ALA group (3.65 ± 2.03 uM) was significantly lower than CON group (4.88 ± 2.84 uM)(p < .05). Plasma glucose in ALA group ( 140.95 ± 14.89 mg‧dL-1) was significantly higer than CON group (130.70 ± 16.11 mg‧dL-1)(p < .05). Erythrocyte SOD activity in ALA group (4461.99 ± 677.22 U‧gHb-1) was significantly lower than CON group (5143.49 ± 554.10 U‧gHb-1)(p < .05). Erythrocyte GPx activity in ALA group (125.72 ± 22.43 kU‧gHb-1) was significantly higher than CON group (59.32 ± 28.20 kU‧gHb-1)(p < .001). Erythrocyte CAT activity in ALA group (24.27 ± 11.32 kU‧gHb-1) was significantly lower than CON group (42.56 ± 10.10 kU‧gHb-1)(p < .001). Conclusion:. Runners of 100-km road race benefited from LA supplement with lowered lipid peroxidation and increased GPx activity during exercise.
Key words:α-lipoic acid, extreme endurance running, oxidative damage

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