本論文係探究於 [Ru(bpy)2(dppz)]2+ 及其衍生物 complexs 對特定 AT-rich與 GC-rich DNA 序列之鏡像選擇性與結合性之研究。前人研究指出 [Ru(bpy)2(dppz)]2+ (dppz = dipyrido [3,2-a:2’,3’-c]phenazine) 是個有名的 “light switch” 分子，因為於非質子溶劑中，經照光後可以發出磷光，但是於質子溶劑水溶液中，磷光會被 H+ 給 quench 掉；且還可經由氧化電子傳遞，作為切斷特定 DNA 序列的試劑，此類 complexes中的 dppz ligand，能夠與 DNA 的鹼基產生 π stacking 而插入於 DNA 鹼基對之間，遮蔽掉溶劑中質子與 dppz ligand 上的 N 作用的可能性，於照光後仍可發出磷光。在本實驗中，我們還測試了另三個衍生物[Ru(dmbpy)2(dppz)]2+、[Ru(Cl2bpy)2(dppz)]2+ 和 [Ru(dCOOHbpy)2(dppz)]2+ complexes。在本實驗中我們以上述 Ru complexes 測試了四條雙股右旋且具迴文對稱的 octamers DNA 序列 (d[CGCATGCG])2、(d[CGCTAGCG])2、(d[TATGCATA])2 和 (d[TATCGATA])2。利用 UV/Vis 變溫吸收光譜、UV/Vis 的滴定、circular dichroism (CD) 變溫吸收光譜和 equilibrium dialysis 實驗，來推論出各別 Ru complexes所偏好的 DNA 序列。實驗結果顯示，我們在 [Ru(bpy)2(dppz)]2+、[Ru(dmbpy)2(dppz)]2+ 和 [Ru(Cl2bpy)2(dppz)]2+ 這三者皆可觀察到，其中 [Ru(dmbpy)2(dppz)]2+ 和 [Ru(Cl2bpy)2(dppz)]2+ 最為明顯強烈。且傾向對 AT-rich 的 DNA 序列作結合。然而 DNA 對 [Ru(bpy)2(dppz)]2+ 兩種 enantiomers 的區分能力不是很好，認為應該是對兩種 enantiomers 的結合能力都不差，卻少了專一性。而 [Ru(dCOOHbpy)2(dppz)]2+ 卻是幾乎沒有結合與插入 DNA 鹼基對中的能力。我們也觀察到了各 complexes 對於 DNA 中 purine 和 pyrimidine 彼此交錯排列的序列，有著較強的結合能力，例如 [Ru(bpy)2(dppz)]2+ 是對 (d[CGCATGCG])2，[Ru(dmbpy)2(dppz)]2+ 和 [Ru(Cl2bpy)2(dppz)]2+ 則是對 (d[TATGCATA])2，而 [Ru(dCOOHbpy)2(dppz)]2+ 因結合能力低無能判讀其序列之選擇性。推論於 ancillary ligand 上修飾推拉電子的取代基，是可以有效改善其 enantioselectivity 和與 DNA 結合的能力，且取代基大小比推拉電子性更強烈相關。未來可以以此為基礎，去設計更具專一性和結合能力的 DNA probes 或是 DNA 切割試劑。

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