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研究生: 郭家瑀
Kuo, Chia-Yu
論文名稱: 以Figaro二氧化碳感測器監測新舊榕樹葉在不同溫度下之呼吸速率
Monitoring of the respiration rate of young and old Marabutan leaves at different temperatures by using a Figaro CO2 sensor
指導教授: 林震煌
Lin, Cheng-Huang
學位類別: 碩士
Master
系所名稱: 化學系
Department of Chemistry
論文出版年: 2019
畢業學年度: 107
語文別: 中文
論文頁數: 56
中文關鍵詞: 呼吸速率非分散性紅外線吸收氣體感測器自組裝二氧化碳感測裝置
英文關鍵詞: respiration rate, NDIR gas sensor, self-assembled carbon dioxide sensing device
DOI URL: http://doi.org/10.6345/NTNU201900114
論文種類: 學術論文
相關次數: 點閱:132下載:0
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  • 以往計算植物呼吸速率的方式為採用氣相層析質譜儀或使用外界的氣體分析儀採集封閉樣品內的氣體進行氧氣的消耗速率以及二氧化碳生成速率的計算。其過程不僅樣品製作繁瑣且有諸多限制,體積的限制下讓氣相層析質譜儀無法隨身攜帶,只能將植物的葉片摘取回實驗室進行測量。且質譜儀的價格要價不斐,其維護成本也不小,諸多原因讓對植物呼吸速率的研究造成阻礙。再者使用氣體分析儀的情況下,大量抽取封閉系統內的氣體更是會影響呼吸作用。
    本研究利用Figaro非分散性紅外線吸收氣體感測器晶片自組裝二氧化碳感測裝置,成功在短時間內觀察到榕樹樹葉於常溫到高溫的範圍下的暗呼吸作用速率。且發現,榕樹的樹葉在新生階段因為需要大量的養分來供給發育所需的能量,所以在常溫階段下(攝氏25度~45度)的呼吸速率會比早已成熟的深綠色老葉快。但到了高溫階段(攝氏45度~70度),新葉不僅呼吸速率較老葉低,也比老葉更早死亡。推測其原因為新葉的厚度較薄,其角質層也較為不完善,內部的呼吸作用相關酵素無法有效受到保護,所以對於高溫的耐受度相對老葉較為低。
    並且本研究開發的感測裝置長24公分、高12公分、寬15公分,且重量僅1.5公斤。方便攜帶的特性只要配合筆記型電腦即可隨地進行植物的呼吸作用速率測量。並且將感測器設計在封閉系統內部減少對呼吸作用造成的影響。

    The traditional way to calculate plant respiration rate was to calculate the rate of oxygen consumption or the rate of carbon dioxide generation using a gas chromatography mass spectrometer or a gas analyzer. However, many reasons hinder the research on the respiration rate of plants. With a big of amount volume, the gas chromatography mass spectrometer can’t be carried around, and only the leaves of the plants can be taken back to the laboratory for measurement. In the case of a gas analyzer, a large amount of gas extracted from the closed system will affect the respiration.
    In this study, a carbon dioxide sensing device was built using a commercially available NIPD gas sensor which was made by Figaro and successfully used to observe the dark respiration rate of Marabutan leaves in the range from normal temperature to high temperature in a short time. It is also found that the Marburatan leaves which are in the born stage have faster respiration rate than the already mature dark green leaves at normal temperature stage (25 degrees to 35 degrees Celsius) because they need energy for development. However, at high temperature stage (35 degrees to 70 degrees Celsius), the new leaves not only have a lower respiratory rate, but also die earlier than the old leaves. It is speculated that the reason is that the thickness of the new leaves is thin, the stratum corneum is also imperfect, and the inner mitochondria cannot be effectively protected, so the tolerance to high temperature is lower than that of the old leaves.
    Moreover, the sensing device developed in this study is 24 cm long, 12 cm high, 15 cm wide, and weighs only 1.5 kg. Easy-to-carry features can be used to measure the rate of respiration of plants with a laptop. And the sensor is designed inside the closed system to reduce the impact on leaves respiration.

    摘要 I Abstract II 目錄 III 圖次 VI 表次 VIII 第一章 緒論 1 1-1研究目的 1 1-2分析物簡介 3 第二章 分析原理及方法 4 2-1非分散性紅外線二氧化碳感測器 4 2-1-1紅外線吸收光譜歷史簡介 4 2-1-2紅外線吸收光譜原理 5 2-1-3非分散性紅外線偵測 6 2-2呼吸作用 8 2-2-1糖解作用 8 2-2-2乙醯輔酶A (Acetyl-CoA)的形成 9 2-2-3檸檬酸循環 9 2-2-4電子傳遞鏈 9 2-呼吸速率 10 2-4影響呼吸速率的因素 10 2-4-1植物種類 11 2-4-2溫度 11 2-4-3氧氣 11 2-4-4光照影響 11 2-4-5葉齡 12 2-4-6水分 12 第三章 儀器與實驗方法 13 3-1實驗儀器 13 3-1-1自組裝二氧化碳氣體感測裝置 13 3-1-2 CDM7160二氧化碳感測器 17 3-1-3葉片呼吸室 19 3-2 裝置控制、資料處理與LabVIEW程式編制 21 3-2-1二氧化碳濃度值的即時監測 21 3-2-2風扇型氣體流動裝置控制 23 3-3資料處理 25 呼吸作用速率 25 3-4儀器設備列表 26 第四章 研究過程與結果討論 28 4-1榕樹葉片選擇與處理 28 4-2實驗流程 29 4-3榕樹葉片呼吸速率觀測 30 4-3-1水浴溫控裝置溫度梯度 30 4-3-2二氧化碳濃度與時間關係曲線 31 4-3-3溫度與呼吸速率關係圖 32 4-4實驗方法2 37 第五章 結論 38 第六章 參考資料 40 創意化學實驗:嵌入式平台的氣體感測器對不同酵母菌在麵糰發酵過程中二氧化碳變化之研究 44

    1. Gammon, R.H., History of carbon dioxide in the atmosphere, in Atmosphere carbon dioxide and the global carbon cycle, J.R. Trabalka, Editor. 1985, United States Department of Energy: Oak Ridge National Laboratory. p. 26-62.
    2. Observatory, M.L. Daily CO2. 2019.
    3. Andrich, G., et al., Aerobic respiration rate of Golden Delicious apples as a function of temperature and PO2. Postharvest Biology and Technology, 1998. 14(1): p. 1-9.
    4. Boysen, L.R., et al., The limits to global-warming mitigation by terrestrial carbon removal. 2017. 5(5): p. 463-474.
    5. Cox, P.M., et al., Acceleration of global warming due to carbon-cycle feedbacks in a coupled climate model. Nature, 2000. 408(6809): p. 184-187.
    6. Huntingford, C., et al., Implications of improved representations of plant respiration in a changing climate. Nature Communications, 2017. 8(1): p. 1602.
    7. Nishiyama, M., et al., Temperature dependence of respiration rates of leaves, 18O-experiments and super-Arrhenius kinetics. Chemical Physics Letters, 2009. 482(4-6): p. 325-329.
    8. Saxe, H., et al., Tree and forest functioning in response to global warming. 2001. 149(3): p. 369-399.
    9. Geronimo, J. and H. Beevers, Effects of Aging and Temperature on Respiratory Metabolism of Green Leaves. Plant Physiology, 1964. 39(5): p. 786.
    10. Zhou, H., et al., Leaf-age effects on temperature responses of photosynthesis and respiration of an alpine oak, Quercus aquifolioides, in southwestern China. Tree Physiology, 2015. 35(11): p. 1236-1248.
    11. Acerbi, F., et al., Novel methodology for the in situ assessment of CO2 production rate and its application to anaerobic ripened cheese. Food Research International, 2015. 78: p. 295-301.
    12. González-Buesa, J. and M.L. Salvador, An Arduino-based low cost device for the measurement of the respiration rates of fruits and vegetables. Computers and Electronics in Agriculture, 2019. 162: p. 14-20.
    13. Nishiyama, M., et al., Mass spectrometric study of the kinetics of O2 consumption and CO2 production by breathing leaves. Chemical Physics Letters, 2009. 470(4-6): p. 332-336.
    14. Barbosa, N.C., R.A. Mendonça Vieira, and E.D. de Resende, Modeling the respiration rate of Golden papayas stored under different atmosphere conditions at room temperature. Postharvest Biology and Technology, 2018. 136: p. 152-160.
    15. Løkke, M.M., et al., Novel Wireless Sensor System for Monitoring Oxygen, Temperature and Respiration Rate of Horticultural Crops Post Harvest. Sensors, 2011. 11(9): p. 8456-8468;.
    16. Mahajan, P.V., A. Luca, and M. Edelenbos, Development of a small and flexible sensor-based respirometer for real-time determination of respiration rate, respiratory quotient and low O2 limit of fresh produce. Computers and Electronics in Agriculture, 2016. 121: p. 347-353.
    17. Germplasm Resources Information Network (GRIN). Agricultural Research Service (ARS), U.S.D.o.A.U. "Ficus microcarpa". 2019; Available from: (May,24,2019).
    18. Aughton, P., The Story of Astronomy. Vol. 256. 2011, New York: Quercus.
    19. Rowan-Robinson, M., Night Vision: Exploring the Infrared Universe. 2013.
    20. Thomas, N.C., The early history of spectroscopy. Journal of Chemical Education, 1991. 68(8).
    21. Lau, W.S., Infrared Characterization for Microelectronics. Infrared Characterization for Microelectronics. 1999, Singapore: hartered Semiconductor Manufacturing Limited.
    22. Peter Atkins, J.d.P., Elements of physical chemistry (5th ed.). 2009, Oxford: Oxford. 459.
    23. Smith, J.G., Chapter 13 Mass Spectrometry and Infrared SpectroscopyOrganic Chemistry 3rd. 2011, New York: McGraw-Hill Companies.
    24. M. Badger, R., A Relation Between Internuclear Distances and Bond Force Constants. The Journal of Chemical Physics, 1934. 2: p. 128-131
    25. Moody, L.M.H.C.J., Experimental organic chemistry: Principles and Practice (Illustrated ed.). 1989, Hoboken: Blackwell Scientific. 292.
    26. Michele Derrick, Dusan Stulik, and J.M. Landry, Infrared Spectroscopy in Conservation Science (Tools for Conservation). 2000, Los Angeles, California: getty Conservation Institute.
    27. Jongwon, K., et al. A study on NDIR-based CO2 sensor to apply remote air quality monitoring system. in 2009 ICCAS-SICE. 2009.
    28. Berg JM, T.J., Stryer L., Chapter 17, Kreb’s Cycle or Citric Acid Cycle or Tricarboxylic Acid Cycle, in Biochemistry. 5th edition. 2002, W H Freeman: New York.
    29. Stenesh, J., The Citric Acid Cycle, in Biochemistry, J. Stenesh, Editor. 1998, Springer US: Boston, MA. p. 273-291.
    30. Beevers, H., 4 - The Role of the Glyoxylate Cycle, in Lipids: Structure and Function, P.K. Stumpf, Editor. 1980, Academic Press. p. 117-130.
    31. Villar, R., A.A. Held, and J. Merino, Comparison of Methods to Estimate Dark Respiration in the Light in Leaves of Two Woody Species. Plant physiology, 1994. 105(1): p. 167-172.
    32. Byrd, G.T., R.F. Sage, and R.H. Brown, A Comparison of Dark Respiration between C(3) and C(4) Plants. Plant physiology, 1992. 100(1): p. 191-198.
    33. Villar, R., A.A. Held, and J. Merino, Dark Leaf Respiration in Light and Darkness of an Evergreen and a Deciduous Plant Species. Plant physiology, 1995. 107(2): p. 421-427.
    34. AMTHOR, J.S., Respiration in a future, higher-CO2 world. 1991. 14(1): p. 13-20.
    35. Sharp, R.E., M.A. Matthews, and J.S. Boyer, Kok effect and the quantum yield of photosynthesis : light partially inhibits dark respiration. Plant physiology, 1984. 75(1): p. 95-101.
    36. Collier, D.E. and B.A. Thibodeau, Changes in respiration and chemical content during autumnal senescence of Populus tremuloides and Quercus rubra leaves. Tree Physiology, 1995. 15(11): p. 759-764.
    37. Crous, K., et al., Light inhibition of leaf respiration in field-grown Eucalyptus saligna in whole-tree chambers under elevated atmospheric CO2 and summer drought. Vol. 35. 2011. 966-81.
    38. Rodríguez-Calcerrada, J., et al., Leaf CO2 efflux is attenuated by acclimation of respiration to heat and drought in a Mediterranean tree. 2011. 25(5): p. 983-995.
    39. Atkin, O.K. and D. Macherel, The crucial role of plant mitochondria in orchestrating drought tolerance. Annals of botany, 2009. 103(4): p. 581-597.
    40. Robinson, P.K., Enzymes: principles and biotechnological applications. Essays in biochemistry, 2015. 59: p. 1-41.
    41. Hatfield, J.L. and J.H. Prueger, Temperature extremes: Effect on plant growth and development. Weather and Climate Extremes, 2015. 10: p. 4-10.
    42. Daniel, R.M. and M.J. Danson, Temperature and the catalytic activity of enzymes: A fresh understanding. FEBS Letters, 2013. 587(17): p. 2738-2743.
    43. Jiang, X., et al., Dynamic Perturbation of the Active Site Determines Reversible Thermal Inactivation in Glycoside Hydrolase Family 12. Journal of Chemical Information and Modeling, 2017. 57(2): p. 288-297.
    44. Peterson, Michelle E., et al., The dependence of enzyme activity on temperature: determination and validation of parameters. Biochemical Journal, 2007. 402(2): p. 331.

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