近年來，許多將無機奈米結構界面與生物系統相連的材料已引起細胞學及生物醫學上廣泛的應用。半導體量子點是目前研究細胞影像中最有希望的新興螢光標記物。在本篇論文中，我們利用水溶性硒化鎘/硫化鋅奈米晶體的螢光，標定人類乳癌細胞 ( MCF 7 )，以研究量子點進入細胞的方式、生物相容性、及在細胞內的光學穩定性。由共軛焦螢光顯微鏡的影像發現，這些細胞內的螢光訊號數天後依然穩定存在，而細胞亦不斷地在進行分裂。為了更進一步確認量子點是否能專一性地標定細胞內的特定胞器，我們將量子點的表面修飾上能辨識細胞核的胜肽以標定細胞核。實驗結果，大多數的螢光訊號對細胞核具有專一性而且比有機染料的螢光更強又穩定。這些實驗結果更促使未來能設計更具功能化的量子點以利活細胞內的蛋白質動態追蹤。

Abstract
Inorganic nanomaterials that interface with biological systems have
recently attracted widespread interests in cellular and biomedical applications.
Semiconductor quantum dots (QDs) are among the most promising emerging
fluorescent labels for cellular imaging. In this thesis, to study the way into
cell、biocompatibility and photostability of QDs, we fluorescently labeled
breast cancer cells ( MCF 7 ) using water-soluble CdSe/ZnS core-shell
nanocrystals. Confocal microscopy images indicated that the cells remained
stably labeled for several days and continued cell division. However, to establish
whether QDs can specifically label organelles at a subcellular level, we used
QDs modified with the nucleus localization signal ( NLS ) peptides to label the
nuclei of the cells. The results showed that much labeling signals are specific for
the nuclei and are brighter and considerably more photostable than comparable
organic dyes. These results encourage the construction of more complex
functional QDs for protein tracking in cell.

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