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研究生： 楊盈盈
Yang Ying-Ying
論文名稱： 線上掃集濃縮－毛細管微胞電動層析法對靶場土壤中 火炸藥之分析研究
Determination of nitroaromatic explosives residue at military shooting ranges using a sweeping-MEKC method
指導教授： 林震煌
Lin, Cheng-Huang
學位類別： 碩士
Master
系所名稱： 化學系
Department of Chemistry
論文出版年： 2008
畢業學年度： 96
語文別： 中文
中文關鍵詞： 火炸藥 、土壤 、三硝基甲苯
英文關鍵詞： TNT, RDX, soil, sweeping-MEKC, EPA Method 8330, explosive
論文種類： 學術論文
相關次數： 點閱：164  下載：0 
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    • 本研究成功的利用毛細管電泳/紫外光吸收偵測法 (CE/UV)，結合線上濃縮的技術，分析多處靶場土壤中所殘留的火炸藥含量。實驗中，以微胞電動層析法(MEKC) 分析14種硝基芳香族及硝基胺類的火炸藥標準品：HMX、RDX、TNT、Tetryl、2,4-DNT、 2,6-DNT、2-NT、3-NT、4-NT、1,3,5-TNB、1,3-DNB、NB、4-Am-4,6-DNT、 4-AM-2,6-DNT等；MEKC模式的偵測極限 (LODs;　S/N = 3) 為1.5 ~ 2.9 μg/mL，為提升偵測靈敏度，利用毛細管電泳掃集法 (sweeping-MEKC) 模式進行偵測，偵測極限可以提升到3.1 ~ 6.5 ng/mL。在真實樣品分析中，檢測金門七處不同靶場土壤之表層土，經過簡單的萃取步驟，在土壤中發現高濃度的 TNT及RDX等二種軍方最常使用的火炸藥。實驗中所開發之分析技術，有助於檢測土壤中殘存的火炸藥，並且可以作為未來相關研究的參考

    This paper describes the process by which nitroaromatic explosives residue in soil sample matrices from military shooting ranges is obtained by means of capillary electrophoresis/UV absorbance. The use of MEKC (micellar electrokinetic chromatography) mode provided the limit of detections (LODs; at S/N = 3) ranging 1.5 ~ 2.9 μg/mL for 14 nitroaromatic and nitramine explosives standards; whereas when the sweeping-MEKC technique was used, the LODs could be improved as much as 3.1 ~ 6.5 ng/mL. A set of 7 soil samples were collected from surface soil at military shooting ranges located at Kinmen County in Taiwan, and the findings showed that RDX and TNT were the explosives residue present at the highest concentrations. This study is useful for determining current levels of explosives residue and to help with appropriate recommendations concerning future site characterization techniques.

    第一章 緒論 1-1 研究目的 1-2 分析物簡介 1-3 火炸藥的分析法 第二章 分析方法及原理0 2-1 毛線管電泳之基本原理 2-1-1 電泳遷移率 2-1-2 電滲流（EOF） 2-1-3 管柱分離效率 2-2 毛細管電泳法的分離模式 2-2-1 毛細管區帶電泳（CZE） 2-2-2 微胞電動層析法（MEKC） 2-3 毛細管線上濃縮技術 2-3-1 毛細管電泳線上掃集法（sweeping-MEKC） 第三章 研究儀器與藥品 3-1 實驗儀器 3-1-1 毛細管電泳儀器 3-1-2 訊號放大器 3-1-3 儀器與周邊設備列表 3-2 實驗藥品 3-2-1 藥品列表 3-2-2 EPA 8330 3-3 分析物的取得與處理 3-3-1 土壤樣品來源 3-2-2 樣品的前處理 第四章 研究過程與結果討論7 4-1 標準品之最佳分析條件的確立 4-1-1 標準品之配置 4-1-2 分析物的吸收光譜 4-1-3 毛細管區帶電泳法 (CZE) 電泳條件探討 4-1-4 微胞電動層析法 (MEKC) 電泳條件探討 4-1-4-1 鹼性MEKC最佳化電泳條件確立 4-1-4-1 酸性MEKC最佳化電泳條件確立 4-1-4-2 不同模式MEKC對分析結果影響探討 4-1-5 線上掃集法 (sweeping-MEKC) 電泳條件探討 4-1-6 以氣相層析儀偵測火炸藥標準品 4-1-6-1 以氣相層析質譜儀偵測 4-1-6-2 以氣相層析儀/火焰離子化偵測器偵測 4-2 EPA 8330最佳分離條件探討 4-2-1 EPA 8330最佳分離條件的確立 4-2-2 不同電泳模式再現性探討 4-2-3 不同電泳模式的堆積效率及偵測極限 4-3 真實樣品的定量分析 4-3-1 金門靶場土壤中火炸藥的定量分析 4-3-2 土壤樣品萃取再現性探討 4-3-3 土壤樣品萃取回收率探討 第五章 結論 參考文獻 附錄

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