利用毛細管電泳/紫外光吸收偵測法（CE/UV），結合線上濃縮的技
術，分析代謝性壓力實驗下，注射去氧葡萄糖（2-deoxy-D-glucose）試
劑前後，老鼠血清中皮質固酮（corticosterone），以及運動前後人體尿
液中氫基皮質酮（17-hydroxycorticosterone）的含量變化。本研究以一
般微胞電動層析法（normal-MEKC）、毛細管電泳掃集法（sweeping-
MEKC）以及陽離子選擇性完全注射掃集法（CSEI-sweep-MEKC），
三種分析方法進行偵測極限的比較，發現normal-MEKC 只能偵測至7
μg/mL（S/N=3），而sweeping-MEKC 和CSEI- sweep-MEKC 可分別達
3 ng/mL 及5 ng/mL（S/N=3）的偵測極限。在真實樣品分析中，檢測
代謝性壓力實驗下，老鼠血清中皮質固酮的平均含量，其中慢性受壓
老鼠濃度為552 ± 152 ng/mL；急性受壓老鼠濃度為375 ± 105 ng/mL，
皆較未受壓老鼠濃度153 ± 109 ng/mL，明顯增高；而運動性壓力實驗
下，人體尿液的檢測，在5~10 分鐘內跑完800 公尺後，人體尿液中微
量氫基皮質酮的平均濃度，男性為137 ± 55 ng/mL、女性為128 ± 25
ng/mL，相較於未跑前所測量的平均濃度，男性63 ± 37 ng/mL，女性
60 ± 20 ng/mL，亦有升高的趨勢。實驗中所研發之分析技術，只需要
極少量的樣品，即可檢測壓力指標荷爾蒙－糖皮質類固醇的濃度，有
助於醫學上用來了解身體對壓力因子的反應機制，做為個體健康狀態
的參考指標，並可應用於醫藥學上，評估各種天然或合成藥物對於減
緩壓力之療效。

The analysis of corticosterone in mouse blood serum (metabolic-stress
experiment) and 17-hydroxycorticosterone in human urine (exercise-stress
experiment) samples by means of capillary electrophoresis/UV absorbance
in conjunction with online sample concentration techniques is described.
The use of normal MEKC had an analyte detection limit of 7 μg/mL (S/N
=3); whereas when online sample concentration methods, including
sweeping-micellar electrokinetic chromatography (sweeping-MEKC)
and cation-selective exhaustive injection-sweep-micellar electrokinetic
chromatography (CSEI-sweep-MEKC) were used, the detection limits
could be improved to 3 and 5 ng/mL, respectively. In the analysis of actual
samples from animal metabolic-stress experiments (39 mouse), chronically
stressed animals showed a higher level (552 ± 152 ng/mL) and acute
stressed animals showed an intermediate level (375 ± 105 ng/mL).
In comparison, normal animals show a lower concentration level of
corticosterone (153 ± 109 ng/mL). In addition, based on a human
exercise-stress experiment (seven volunteers), the acute stressed humans
(after exercise, 800m of running) show a higher concentration of
17-hydroxycorticosterone (137 ± 55 ng/mL for males; 128 ± 25 ng/mL for
females) and the non-stressed humans (before exercise) show a lower
concentration (63 ± 37 ng/mL for male; 60 ± 20 ng/mL for female),
respectively. Furthermore, this method requires only small amounts of
plasma and could be used as a routine tool in pharmacological studies
dealing with different types of drugs, whether derived from natural herbs or
by synthesis.

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