微流道技術在現今的社會中發展日益成熟，它能結合包括生物、工程、化學、物理、微加工等不同領域的技術。其特色為可微控粒子 ，精度可達微米等級。並結合磁粒子技術，利用流道深寬限制、給予場到分離特定粒子的目，可應用於細胞篩選、重組，生物分析等。並且利用微觀尺度下流體力學特性，可增加樣本反應速率。高分子晶片有許多製程方式包含熱壓法、雷射雕刻光阻轉印、化學侵蝕等。 本實驗使用CO2雷射雕刻將0.1 mm壓克力薄片刻穿，成功開發一套以壓克力材料製作之夾心式微流道樣品混合晶片，並以紅墨水與進行混合測試，使用Image J建立出一套完整的影像之數據分析流程，實驗 結果 與菲克定理相互驗證，得本製程製作出之 寬度為 200 μm之微流道晶片 微流道晶片可在2~2.4秒間達95%以上之混合效果。未來可應用於增進本實驗室不同抗體原之結合效率。

Microfluidic technology is increasingly mature in nowadays, and it can combine technologies in different fields including biology, engineering, chemistry, physics, and micromachining. It feature is what can micro-controlled in particles with micron precision. Combined with the magnetic particle technology, it can use of limit the depth and width of the channel or give magnetic field to achieve the purpose of separating the specific particles can be applied to cell screening, recombination, biological analysis. And using the hydrodynamic properties at the microscopic scale,
the sample reaction rate can be increased. Polymer wafers have many processes, including hot pressing, laser engraving, photoresist transfer and chemical etching. Our experiment uses laser engraving to penetrate the 0.1 mm acrylic and a sandwich microfluidic mixer wafer made of acrylic material was completed. We mixed red ink and water by our microfluidic, and use Image J to make a complete analysis process. We verify the analysis results with Fick’s theorem and verify that the microfluidic with a width of 200 μm produced in our experiment with more than 95% mixed effect in 2~2.4 seconds.

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