研究生: |
李政學 Cheng-Hsueh Lee |
---|---|
論文名稱: |
絨弄蝶屬之分子親緣與分類關係探討 Molecular phylogeny and systematic clarification of the genus Hasora (Lepidoptera: Hesperiidae) |
指導教授: |
徐堉峰
Hsu, Yu-Feng 千葉秀幸 Hideyuki Chiba |
學位類別: |
碩士 Master |
系所名稱: |
生命科學系 Department of Life Science |
論文出版年: | 2012 |
畢業學年度: | 100 |
語文別: | 英文 |
論文頁數: | 45 |
中文關鍵詞: | 分子系統學 、種群 、東洋區 、澳洲區 |
英文關鍵詞: | molecular systematics, species group, Oriental Region, Australian Region, Awl |
論文種類: | 學術論文 |
相關次數: | 點閱:134 下載:1 |
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絨弄蝶屬 (Hasora)為鱗翅目 (Lepidoptera)弄蝶科 (Hesperiidae)大弄蝶亞科 (Coeliadinae)之中大型弄蝶,主要分布於印度至澳洲間,是大弄蝶亞科中種數最多的類群,在目前分類共有約30種,可被分為6-7個種群。本屬傳統分群與分類主要依據成蝶翅紋與交尾器特徵,然而本屬在部分物種的分類上經常缺乏共識,而過去以交尾器與翅紋相似性為依據進行之分群是否能合理反映絨弄蝶之親緣也仍未釐清。本研究以粒線體DNA之COI, COII 片段與核DNA之Ef-1a片段重建絨弄蝶屬之分子親緣關係,比較其與de Jong提出之形態親緣是否相符,並用以檢測絨弄蝶屬傳統分群架構的合理性與釐清本屬的分類問題。本研究內外群共採樣27 種與68隻個體,在分群關係方面支持 Chiba 所認定的discolor-group, celaenus-group, vitta-group與thridas-group 以及Evans所認定的 discolor-group與 thridas-group為單系群。而本研究也支持將原屬於chromus-group的 H. schoenherr獨立為 schoenherr-group。 種級關係方面, H. mavis 與 H. leucospila 為同種之假說受強烈支持,而H. vitta則可被分為三個種級分類群。
The skipper genus Hasora (Lepidoptera: Hesperiidae: Coeliadinae), which consists of approximately 30 species classified into 6-7 groups, is the largest genus of the subfamily Coeliadinae and is distributed throughout the Indo-Australian region. Currently recognized species groups and taxonomy of Hasora are mainly based on characteristics of the wing patterns and the male genetalia, and there have been some disagreements in taxonomical treatments. To date it has not been tested if the species groups based on morphological characters correspond to the phylogeny of Hasora. The objective of this research is to reconstruct the molecular phylogeny of Hasora using the sequences of mitochondrial COI, COII region and nuclear Ef-1a region, and to test if the morphological phylogeny proposed by de Jong is consistent with the molecular phylogeny, and if the species groups defined by Evans and Chiba are monophyletic. The relationships of some taxonomically controversial taxa are also investigated. Total of 22 ingroup species, 63 ingroup individuals and 5 outgroup genus were sampled, and the monophyly of discolor-group, celaenus-group, vitta-group and thridas-group sensu Chiba as well as discolor-group and thridas-group sensu Evans were supported respectively. The present study also suggested that H. schoenherr, a species formerly assigned to chromus-group, should be placed to a schoenherr-group proposed herein. For species-level relationships, the conspecific relationship between H. mavis and H. leucospila is strongly supported, and H. vitta may be divided into three different species level taxa.
Bascombe MJ, G Johnston, and FS Bascombe. 1999. The Butterflies of Hong Kong. London: Academic Press. pp.79.
Braby MF. 2000. Butterflies of Australia: Their identification, biology and distribution. Vol.1. Collingwood: CSIRO. pp. 79-84.
Canfield MR, E Greene, CS Moreau, N Chen and NE Pierce. 2008. Exploring phenotypic plasticity and biogeography in emerald moths: A phylogeny of the genus Nemoria (Lepidoptera: Geometridae). Mol. Phylogenet. Evol. 49(2): 477-487.
Caterino MS and FAH Sperling. 1999. Papilio phylogeny based on mitochondrial cytochrome oxidase I and II genes. Mol. Phylogenet. Evol. 11: 122-137.
Chiba H. 2009. A revision of the subfamily Coeliadinae of the world (Lepidoptera: Hesperiidae). Bull. Kitakyushu Mus. Nat. Hist. Hum. Hist. A 7: 1-102.
Edgar RC. 2004. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32(5): 1792-97.
Eliot JN. 1992. The butterflies of the Malay Peninsula. Fourth edition. Kuala Lumpur: Malayan Nature Society.
Elwes HJ and J Edwards. 1897. A revision of the Oriental Hesperiidae. Trans. zool. Soc. Lond. 14:101-324.
Evans WH.1934. Indo-Australian Hesperiidae; Descriptions of new genara, species and subspecies. Entomologist 67:33-36.
Evans WH. 1949. A catalogue of the Hesperiidae from Europe, Asia and Australia in the British museum (Natural History). London: The British Museum. pp. 55-72.
Goloboff P, J Farris and K Nixon. 2008. TNT, a free program for phylogenetic analysis. Cladistics 24: 774–786
Guindon S, JF Dufayard, V Lefort, M Anisimova, W Hordijk and O Gascuel. 2010. New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Systematic Biology 59(3):307-321.
Heled J and AJ Drummond. 2010. Bayesian inference of species trees from multilocus data. Mol. Biol. Evol. 27: 570-580.
Hsu YF, H Tsukiyama and H Chiba. 2005. Hasora anura de Nicéville from Taiwan (Lepidoptera: Hesperiidae: Coeliadinae) representing a new subspecies Endemic to the island. Zoological Studies 44(2): 200-209.
Hundsdoerfer AK, D Rubinoff , M Attié, M Wink and IJ Kitching. 2009. A revised molecular phylogeny of the globally distributed hawkmoth genus Hyles (Lepidoptera: Sphingidae), based on mitochondrial and nuclear DNA sequences. Mol. Phylogenet. Evol. 52(3): 852-865.
Igarashi S, H Fukuda. 2000. The life histories of Asian butterflies. Vol. II. Tokyo: Tokai University Press. pp. 631-632. pl. 335-336. de Jong R.1982. Neue und wenig bekannte Taxa der Gattung Hasora Moore (Lep.: Hesperiidae). Entomologische Zeitschrift 92(4): 33-40.
Drummond AJ and Rambaut A. 2007. BEAST: Bayesian evolutionary analysis by sampling trees. BMC Evol. Biol. 7: 214.
de Jong R. 2007. Estimating time and space in the evolution of the Lepidoptera. Tijdschr. Entomol. 150: 319-346.
de Jong R and CG Treadaway.1993. The Hesperiidae (Lepidoptera) of the Philippines. Zool. Verh. Leiden 288:1-125.
de Jong R and CG Treadaway. 2007. Hesperiidae of the Philippine islands. Butterflies of the World. Supplement 15:1-72.
Kandul NP, VA Lukhtanov, AV Dantchenko, JWS Coleman, CH Sekercioglu, D Haig1 and NE Pierce. 2004. Phylogeny of Agrodiaetus Hübner 1822 (Lepidoptera: Lycaenidae) inferred from mtDNA sequences of COI and COII and nuclear sequences of EF1-α: Karyotype diversification and species radiation. Syst Biol 53(2): 278-298.
Kitamura M. 2002. Butterflies from the southwest side slope of Mt. Banahaw, mid-south Luzon, Philippines (7). Hesperiidae Part II, Satyridae. Butterflies 34: 43-57. (in Japanese)
Knowles LL and BC Carstens. 2007. Delimiting Species without Monophyletic Gene Trees. Syst Biol 56(6): 887-895.
Lin CH. 2010. Reconstruction of phylogeny within the tribe Theclini (Lepidoptera, Lycaenidae, Theclinae) and the evolution of host plant use. Master Dissertation, NTNU, Taipei.
Maddison WP and LL Knowles. 2006. Inferring phylogeny despite incomplete lineage sorting. Syst Biol 55(1): 21-30.
Maruyama K. 1991. Butterfl ies of Borneo. Vol. 2, No. 2, Hesperiidae. Tokyo: Tobishima. pp. 89.
Monastyrskii A and AL Devyatkin. 2003. Butterflies of Vietnam (an illustrated checklist). Devon: NHBS. pp. 42.
Posada D. 2008. jModelTest: Phylogenetic model averaging. Mol. Biol. Evol. 25: 1253-1256.
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, and Kumar S. 2011. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol. doi: 10.1093/molbev/msr121.
Tanikawa-Dodo Y, T Saigusa, H Chiba, T Nishiyama, T Hirowatari, M Ishii, T Yagi, M Hasebe, and H Mohri. 2008. Molecular phylogeny of Japanese skippers (Lepidoptera, Hesperiidae) baed on mitochondrial ND5 and CO1 gene sequences. Trans. Lepid. Soc. 59: 29-41.
Tsukiyama H, H Chiba, and T Fujioka. 1997. Japanese butterflies and their relatives in the world. Vol. I. Tokyo: Shuppan Geijyutsu Sha. pp. 57-60.(in Japanese with English summary)
Warren AD, JR Ogawa, and AVZ Brower. 2008. Phylogenetic relationships of subfamilies and circumscription of tribes in the family Hesperiidae (Lepidoptera: Hesperioidea). Cladistics 24:642–676.
Warren AD, JR Ogawa, and AVZ Brower. 2009. Revised classification of the family Hesperiidae (Lepidoptera: Hesperioidea) based on combined molecular and morphological data. Systematic Entomology 34: 467–523.
Wiemers M and K Fiedler. 2007. Does the DNA barcoding gap exist?-A case study in blue butterflies (Lepidoptera: Lycaenidae). Front Zool 4: 8.
Wu LW. 2010. Elucidating origins of the Cycad Blue (Chilades pandava): a threat to cycad plants worldwide, with a discussion on the evolution of Cycas-feeding behavior. Ph. D Dissertation, NTNU, Taipei.