苯乙胺及苯乙酸分別為人體中重要的神經傳導物質以及代謝產物，其中尿液中的苯乙酸為偵測苯丙酮尿症患者的重要指標。然而尿液樣品所產生的基質干擾，抑制了細胞中環腺苷酸-CRP 轉錄結合位點系統，造成全細胞生物感測器的偵測異常。在此，先在標準培養液中模擬出碳源代謝抑制效應，並透過額外添加環腺苷酸、2-脫氧葡萄糖及更換培養液氮源的方式解除其效應，再將此優化的解抑制條件運用在尿液樣品中，達到更廣的檢測應用。

Phenylethylamine and phenylacetic acid are important metabolites in the human body. Specifcally, urinary phenylacetic acid is a vital indicator for the diagnosis of phenylketonuria. However, the matrix effect generated by the urinary glucose represses the cyclic adenosine monophosphate-CRP transcriptional binding in the cell. In this study, the carbon catabolite repression is first simulated in the standard culture media, and it is released by adding cyclic adenosine monophosphate, 2-Deoxy-D glucose, and replacing nitrogen sources of the culture media. Finally, the optimization of released conditions is applied to the urine samples to achieve a better detection application.

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