生物體中細胞色素P450的催化循環過程中，推測會有鐵超氧化物、鐵過氧化物和鐵過氧氫化物之中間體生成，其中鐵超氧化物為重要的中間體。本研究利用三氮二氧五牙基H2(BDPP)與二價鐵金屬離子反應形成前驅物FeII(BDPP) (1)。在-70 oC下，將氧氣通入前驅物1會形成FeIII(BDPP)(O2˙) (2)；若持續通入氧氣五個小時，則會生成(FeIII(BDPP))2(μ - O2) (3)。 將其樣品偵測氣相色譜法-質譜聯用(Gas chromatography–mass spectrometry)，則會得到產物2-羥基四氫呋喃(2-hydroxy-tetrahydro-furan)與γ-丁內酯(γ-butyrolactone)。和提前加入的內標準品十二烷(Dodecane)相比較，則可得出產率。

living organism, the catalytic process of cytochrome P450 is presumed to produce iron superoxo , iron peroxo and iron hydroperoxo intermediates. Among them iron superoxo is an the first critical intermediate. In this study, N3O2 petadentate ligand, H2(BDPP), was reacted with FeCl2 to form FeII(BDPP) (1) precursor. At -70 oC, FeII(BDPP) (1) was reacted with dioxygen to produce FeIII(BDPP)(OO˙) (2). If dioxygen was continuously supplied for five hours, (FeIII(BDPP))2(μ - O2) (3) was formed.
From sample detection of gas chromatography-mass spectrometry, the product 2-hydroxytetrahydrofuran and γ-butyrolactone are obtained. The yield is obtained in comparison with the internal standard dodecane , which was added in advance.

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