本研究是利用含鐵黏土(Montmorillonite K1O)修飾電極催化發
光胺(Luminol)以化學發光(chemiluminescence, CL)形式偵測過氧化
氫，並藉以間接偵測葡萄糖。發光胺氧化時可放射λmax=430nm之螢
光，其發光強度與pH值、外加電壓和發光胺濃度有關。實驗結果顯
示最佳化條件為pH=10、E=0.8V vs.SCE，並在發光胺濃度與
過氧化氫濃度之比值為1時達到極限。利用此方法偵測過氧化氫或葡
萄糖並不會受到氧氣干擾。若將葡萄糖氧化酵素(Glucose Oxidase，
GOx)固定於黏土電極表面上，可藉由GOx轉化葡萄糖以產生過氧化氫
而間接達到偵測葡萄糖之目的，偵測極限為5x1O(-5)M，線性範圍為
10(-2)-10(-4)M。此外，利用交流阻抗研究發光胺與過氧化氫反應時電
子傳遞之動力學，結果顯示當濃度比值接近1時，系統的電子轉移阻
抗可達最小值。

Clay modified electrodes were prepared with montmonllonite K1O and characterized in their abihty to enhance the electrogenerated cheimiuminescence (ECL) in a basic solution containing luminol and hydrogen peroxide. Montmorillonite K1O proved to be an effective catalyst for such a purpose, according to the anodic current and ECL intensity measured with the electrodes in the presence and absence of and the clay particles. Under optimum conditions (pH 10 and 0.8V vs.SCE), the ECL was enhanced by a factor often. Control experiments showed the enhanced ECL is likely due to a facihtation in the oxidation of luminol and H2O2 by the iron species contained in the clay. Double-step potential techniques suggested that superoxide ion is very likely to be the key reactive oxygen species involved in the day-enhanced ECL reaction. Based on these clay modified electrode, a GOx enzyme electrode was constructed for the detection of glucose. Although the electrode sensitivity decreased by ca. 30% after 15 days, a linear calibration curve was attained, covering the concentration of glucose from 100 μM to 10 mM with a detection limit at ca. 50 μM at pH 10. In this work, AC impedance techniques were also employed to characterize the electrode behavior, suggesting that the exchange current through the electrode surface reached a maximum value as the ratio of [glucose] to [H2O2] appraching 1, agreeing well with the results obtained by linear sweeping and chronoamperometrie techniques.