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研究生: 謝惠婷
Hui-Ting Hsieh
論文名稱: 台東蘇鐵族群生態及遺傳變異之 研究
Studies on pupulation ecology and genetic variability of Cycas taitungensis
指導教授: 黃生
Huang, Shong
學位類別: 碩士
Master
系所名稱: 生命科學系
Department of Life Science
畢業學年度: 87
語文別: 中文
論文頁數: 113
中文關鍵詞: 台東蘇鐵族群生態遺傳結構核糖體DNA
英文關鍵詞: Cycas taitungensis, population ecology, genetic structure, rDNA
論文種類: 學術論文
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  • 台東蘇鐵 (Cycas taitungensis Shen, Hill, Tsou & Chen) 僅分佈在台灣東南部海岸山脈和鹿野溪中游陡峭山坡上,研究其族群生態及遺傳變異的結果顯示台東蘇鐵在人為干擾區域有年齡結構不均勻的現象,在遺傳變異上則顯示族群內變異極大,而亞族群間的分化程度低。
    本研究共分兩部份,一是族群生態研究:在台東蘇鐵紅葉村自然保留區內進行台東蘇鐵的每木調查和生態研究,所獲之結果顯示成株雌雄性比略為1.7:1,結種率約45﹪,樹高與葉片數在幼期具相關性,若以樹高為指標,進行族群內的年齡結構評估,則發現中齡植株甚少,年齡結構不均勻。野外觀察資料顯示,成熟種子遭盜採殆盡,小型幼樹亦遭採集,保留區內人為干擾嚴重。
    在遺傳變異方面的研究則以分子序列技術序列出細胞核核糖體DNA的ITS區域共77片斷,應用支序系統學 (Cladistics) 原理,經由比對分析序列,評估台東蘇鐵族群內的歧異度,發現此一古老物種在族群內個體間分子層次上保有極大的變異度 (π=0.17),亞族群間並無基因分化,例如相對山坡上之亞族群,各山坡與分佈隔離之族群比較分別為FST =0.002、0.003、0.006。此高度變異的原因可能源自有性生殖的基因重組,由於台東蘇鐵的種子主要以重力及動物攜帶傳播,且為昆蟲傳粉,亞族群間無分化的幾個原因中,最可能的原因為台東蘇鐵原為連續分佈的族群,今日之隔離分佈係近代所發生,基因流傳中斷不久,兩族群間尚未有足夠的時間產生遺傳上的分化。本研究的結論是分佈在不同坡向及不同地點的台東蘇鐵在生態上呈現顯著的差異,但在遺傳上並沒有分化,微環境的不同僅影響了台東蘇鐵的生長和 (或) 繁殖,尚未影響到它的族群遺傳結構。

    Cycas taitungensis is an endemic species which is restricted to the riverside along Lu-Yei River and the mountainous area at the eastern coastal range in Taiwan. The thesis composed of two parts: the first included ecological study of C. taitungensis and the second genetic variability of C. taitungensis population. In ecological study, the age structure of the population habituated in the disturbed area is unequilibriated. The genetic study showed that the nucleotide diversity within populations is high.
    The enumeration measurment included (1) the amount of seed production per year, (2) one-year survival rate of seedling, (3) sex ratio and age structure and (4) the annual foliage leaf production. Three study plots and one 200-meter transect were arbitrary established in the natural reserve area. The results indicated that the field sex ratio was 1.7:1, the seed reproduction rate c.a. 45%, and one-year seedling survival rate in the range of 2-17%. Also, the numbers of foliage leaves were found positively correlated with the size of the plants. In the disturbed area, the age structure shows that the numbers of mid-age cycads were reduced, that implied the plants have been unlawfully deforested.
    In the genetic variability study, internal transcribed spacer (ITS)region of ribosomal DNA from 50 individuals were amplified, cloned and 77 sequences were obtained. Based on nucleic acid information, the population phylogeny trees were constructed. An unexpected high nucleotide diversity (0.17) among the 77 sequences was detected. However, no genetic differentiation between subpopulations(FST=0.002-0.006)was found, which is in agreement with allozyme analysis. A high genetic polymorphism can be accounted for the intramolecular recombination. The seed dispersal of C. taitungensis is known by gravity and possibly by insect pollination. Since the gene flow between the plants is weak, the poor differentiation between the subpopulation may be habitat fragmentation. Besides, the effect of environment factors was more important in the ecology of C. taitungensis than in genetic variability.

    目 錄 目錄 Ⅰ 圖次 Ⅱ 表次及附錄 Ⅲ 中文摘要 Ⅳ 英文摘要 Ⅴ 壹、前言 1 貳、文獻回顧 5 一、台東蘇鐵的分類地位、生育地、形態特徵及其生殖機制 5 二、蘇鐵類植物的相關研究 10 三、核糖體DNA的相關研究 13 四、族群遺傳與演化 18 參、材料與方法 20 一、台東蘇鐵的生態研究 20 (一)台東蘇鐵每木調查 20 (二)族群數量統計 20 二、台東蘇鐵族群內變異之研究 22 三、統計分析 29 肆、結果 33 一、台東蘇鐵的生態研究 33 (一)每木調查結果 33 (二)存活率估算 37 二、族群內遺傳變異之研究------------------------------------------------- 38 (一)台東蘇鐵核糖體DNA的ITS的序列的多態性 39 (二)族群內遺傳變異及亞族群間遺傳分化的分析 42 (三)親緣樹的建構 45 伍、討論 49 一、台東蘇鐵的族群生態變異之探討---------------------------------------- 49 二、台東蘇鐵族群內遺傳變異之研究-------------------------------------- 52 (一)遺傳變異的生成及維持機制----------------------------------------- 52 (二)低的族群遺傳分化,高的個體內遺傳歧異度 57 陸、結論 60 柒、參考文獻 61 捌、附錄 69

    王玉忠和陳家瑞,1995。中國蘇鐵屬植物的葉表皮特徵及其分類學意義。植物學通報,12:47-51。
    王定躍,1996。中國蘇鐵屬的分類研究,王發祥和梁惠波主編:中國蘇鐵。廣東科技出版社,廣東,295頁。
    李鎮宇,1993。紅檜五個族群同功酵素變異之研究。國立台灣大學森林學研究所碩士論文。
    呂勝由,1996。台灣稀有及瀕危植物之分級彩色圖鑑 (I)。農委會,2-20頁。
    林則桐和邱文良, 1990。公告自然保留區之值被調查。農委會,2-20頁。
    林務局關山林管處研究報告,1979。台灣蘇鐵保護區植物生態之研究。
    柳 榗、楊遠波、呂勝由、林則桐及邱文良,1987。台灣稀有植物群落調查(II)。農委會,16-17頁。
    黃 生,1991。基因流傳與族群分化。生物科學34 (2): 19-32頁。
    黃 生,1992。基因浮動-兼談稀有物種的保育。生物科學 35 (1): 1-7頁。
    鄭宗元、胡大維和徐國士,1975。台灣蘇鐵天然林的發現。中華林學季刊8(2):37-39。
    陳瑞英和周德源,1987。膠體電泳分析DNA片段。電泳分離技術研討會論文集9:87-91。
    陳佳元,1974。由三角點檢測證實台灣海岸山脈向北北東移動。台灣省地質調查所彙刊,24:119-123.
    陳家瑞,1995。現存蘇鐵目植物的分類。植物學通報,12:6--9。
    郭文礫,1978。台灣農業氣候區域研究。中央氣象局編印,170頁。
    孫于卿,1998。台東蘇鐵族群遺傳結構分析。國立台灣大學森林學系研究所碩士論文,1-50頁。
    葉慶龍和范貴珠,1996。海岸山脈台灣蘇鐵自然保護區之研究。台灣省農林廳林務局台東管理處。
    潘富俊,1997。自然保留區經營管理手冊。農委會,61-64頁。
    劉棠瑞和廖日京,1981。樹木學上冊。台灣商務印書館發行,666頁。
    劉棠瑞和蘇鴻傑,1992。森林植物生態學。台灣商務印書館,324-327頁。
    劉業經、呂福原和歐辰雄,1994。台灣樹木誌。國立中學農學院出版委員會,925頁。
    Appels ,R., W. L. Gerlach, E. S. Dennis, H. Swift and W. J. Peacock, 1980, Molecular and chromosomal organization of DNA sequence coding for the ribosomal RNAs in cereals. Chromosoma 78: 293-311.
    Archie, J. W., 1989, Homoplasy excess ratios: new indices for measuring levels of homoplasy in phylogenetics systemics and a critique of the consistency index. Syst. Zool. 38: 253-269.
    Arnheim,N. and H. Erlich, 1992, Polymerase chain reaction strategy. Annu. Rev. Biochem.. 61: 131-156.
    Baldwin, B. G., 1992, Phylogenetic utility of the internal transcribed spacers of nuclear ribosomal DNA in plants: An example from the Compositae. Molec. Phylogenetics Evol. 1: 3 - 16.
    Baldwin, B. G., M. J. Sanderson, J. M. Poter, M. F. Wojciechowski, C. S. Campbell and M. J. Donoghue, 1995, The ITS region of nuclear ribosomal DNA: A valuable source of evidence on angiosperm phylogeny. Ann. Miss. Bot. Gard. 82: 247-277.
    Barker, R. F., N. P. Harberd, M. G. Jarvis and R. B. Flavell, 1988, Structure and evolution of the intergenic region in a ribosomal DNA repeat unit of Wheat. J. Mol. Biol. 201: 1-17.
    Barrett, S. C. H. and J. R. Kohn, 1991, Genetics and evolutionary consequences of small population size in plants: Implications for conservation. In D. Folk and K. E. Holsinger (eds), Genetics and conservation of rare plants , pp.587. Oxford Univ. Press..
    Battie, A., 1978, Plant-animal interactions affections affecting gene flow in Viola. In A. J. Kichards (eds), The pollination of flowers by insects, pp. 151-164. Academic Press, Linnean Society Symposium Series, #6, London.
    Beattie, A., 1978, Plant-animal interactions affecting gene flow in Viola. In A. J. Kichards (ed.), The pollination of flowers by insects, pp. 151-164. Academic Press, Linnean Society Symposium Series, London.
    Byrne, M. and H. James, 1991, Genetic diversity in the cycad Macrozamia riedlei. Heredity 67: 35-39.
    Chamberlain, C. J., 1919, The living Cycads. University of Chicago Press, Chicago.
    Chamberlain, C. J., 1935, Gymnosperms: Structure and evolution. University of Chicago Press, Chicago.
    Chiang, T. Y., B. A. Schaal and C. I. Peng, 1998, Universal primers for amplification and sequencing a noncoding spacer between atpB and rbcL genes of chloroplast DNA. Bot. Bull. Acad. Sin. 39: 245-250.
    Cole, C. T. and D. D. Biesboer, 1992, Monomorphism, reduced gene flow, and cleistogamy in rare and common species of Lespedeza (Fabaceae). Amer. J. Bot. 79 (5): 567-575.
    Dehgan, B. and C. K. K. H. Yuen, 1983, Seed morphyology to dispersal, evolution, and propagation of Cycas L. Bot. Gaz. 144 (3): 412-418.
    De Laubenfels, D. J. and F. Adema, 1998, A taxonomic revision of the genea Cycas and Epicycas gen. nov. (Cycadaceae). Blumea, 43: 351 - 400.
    D'Ovidio, R., 1992, Nucleotide sequence of a 5.8S rDNA gene and of the internal transcribed spacers from Populus deltoides. Plant Mol. Biol. 19: 1069-1072.
    Ellstrand, N. C., R. Ornduff and J. M. Clegg, 1990, Genetic structure of the Australian cycad, Macrozamia communis (Zamiaceae). Amer. J. Bot. 77 (5): 677-681.
    Ellstrand, N. C. and D. R. Elam, 1993, Population genetic consequences of small population size: implication for plant conservation. Annu. Rev. Ecol. Syst. 24: 217-241.
    Endler, J. A., 1973, Gene flow and population differentiation. Science 179: 243-250.
    Farris, J. S., 1989, The retention index and homoplasy excess. Syst. Zool. 38: 406-407.
    Frankel, O. H. and M. E. Soule, 1981, Conservation and evolution. Cambridge Univ. Press. pp.327.
    Gonzalez, I. L., C. Chambers, J. L.Gorski, D. Stambolian, R. D. Schmickel, and J. E. Sylverster, 1990a, Ribosomal RNA sequences and hominid phylogeny. Moil. Biol. Evol. 7 (3): 203-219.
    Gonzalez, I. L., C. Chambers, J. L. Gorski, D. Stambolian, R. D. Schmickel and J. E. Sylverster, 1990b, Sequence and structure correlation of human ribosomal transcribed spacers. J. Mol. Biol. 212: 27-35.
    Gottlieb, L. D., 1977, Electrophoretic evidence and plant systematics. Ann. Miss. Bot. Gard. 64: 161-180.
    Govindaraju, D. R. and C. A. Cullis, 1992, Ribosomal DNA variation among population of a pinus rigida Milli (pitch pine) ecosystem :I. Distribution of Copy number. Heredity 69: 133-140.
    Hamrick, J. L., 1989, Isozymes and the analysis of genetic structure in plant population. In D. E. Soltis and P. S. Soltis (eds.), Isozymes in plant biology, pp. 87-105. Dioscorides press, Portland.
    Hamrick, J. L. and M. J. W. Godt, 1989, Allozyme diversity in plant species. In: A. D. H. Brown, M. T. Clegg, A. L. Kahler and B. S. Wies (eds), Plant population genetic, breeding and genetic resources.pp43-63. Sinaure Association Inc, Sunderland, Mass.
    Hamrick, J. L., M. J. Godt and S. L. Sherman-Broyles, 1992, Factors influencing levels of genetic diversity in woody plant species. In Adams, W. T., S. H. Strauss, D. L. Copes and A. G. Griffin (eds), Population genetics of forest trees. pp.95-124. Kluwer Academic Publishers.
    Hamby, R. K., and E. A. Zimmer, 1988, Ribosomal RNA sequences for inferring phylogeny within the grass family (Poaceae). Plant Syst. Evol. 160: 29-37.
    Hartl, D. L. and A. G. Clerk, 1989, Principle of population Genetics, 2nd. Ed. Sinauer Associates, Inc., Sounderland. Mass.
    Heywood, J. S., 1991, Spacial analysis of genetic variation in plant population. Ann. Rev. Ecol. Syst. 22: 335-355.
    Hills, D. M. and M. T. Dixon, 1991, Ribosomal DNA: Molecular Evolution and Phylogenetic Inference. The Quarterly Rev. Biol. 66 (4): 411 - 446.
    Hizume, M. 1995, Physical Mapping of 5S rRNA Genes in Cycas revoluta. Cytologia. 60: 389-393.
    Holdridge, L. R., 1967, Life Zone Ecology. Tropical Science Center Press.
    Hsaio, C., N. J. Chatteron, K. H. Asay and K. B. Jensen, 1993, Phylogenetic relationships of ten grass species inferred from sequences of the internal transcribed spacer region in nuclear ribosomal DNA. (unpublished).
    Hudson, R. R., M. Slatkin and W. P. Maddison, 1992, Estimation of levels of gene flow from DNA sequence data. Genetics 132: 583-589.
    Jones, D. L. , 1993, Cycads of the world. National Library of Australia Press.
    Jupe, E. R., R. L. Chapman and E. A. Zimmer, 1988, Nuclear ribosomal rNA genes and algal phylogeny - The Chlamydomonas example. Biol. Sys. 21: 223 - 230.
    Johnson, L. A. S., 1959, The families of cycads and the Zamiaceae of Australia. Proceeding of the Linnean Society of New Wales 84 (1): 64-117.
    Karvonen, P. and O. Savolainen, 1993, Variation and inheritance of ribosomal DNA in Pinus sylvestris L. (Scots pine). Heredity 71: 614-622.
    Karvonen, P., A. E. Szmidt and O. Savolainen, 1994, Length variation in the internal transcribed spacer of ribosomal DNA in Picea abies and related species.Theor. Appl. Genetica 89: 969-974.
    Karvonen, P., M. Karjalainen and O. Savolainen, 1993, Ribosomal RNA genes in Scots pine(Pinus sylvestris L.):chromosomal organization and structure. Genetica 88: 59-68.
    Kato, A., T. Nakajima, J. Yamashita, K. Yakura and S. Tanifuji, 1990, The structure of the large spacer region of the rDNA in Vicia faba and Pisum sativum. Plant Moil. Bio. 14: 983-993.
    Kis, T., M. Kis, S. Abel and F. Solymosy, 1988, Nucleotide sequence through the 17S-25S spacer region from tomato rDNA. Nucleic Acids Res. 16: 7179-7179.
    Kimura, M., 1983, The neutral throry of molecular evolution . Cambridge University Press, Cambridge.
    Kumar, S., K. Tamura and M. Nei, 1993, MEGA: molecular evolutionary genetics analysis, version 1.01. The Pennsylvania State University Park, PA16802.
    Li, H. L., 1975, Cycadaceae. In Huang, T. C. (eds), Flora of Taiwan vol. I. pp.496-498. Edit Comm. Fl. Taiwan, Dept. Bot. Natl. Taiwan Univ.
    Li, H. L., 1994, Cycadaceae. In Huang, T. C. (eds), Flora of Taiwan vol. I. pp.546-549. Edit Comm. F2. Taiwan, Dept. Bot. Natl. Taiwan Univ.
    Liston, A., W. A. Robinson and J. M. Oliphant, 1996, Length variation in the ribosomal DNA internal transcribed spacer of non-flowering seed plants. Syst. Bot. 21: 109-120.
    Loveless, M. D. and J. L. Hamrick, 1984, Ecological determinants of genetic structure in plant populations. Ann. Rev. Ecol. Syst. 15: 65-95.
    Martin , A. P. and S. R. Palumbi, 1993, body size, metabolic rate, generation time, and the molecular clock. Proc. Natl. Acad. Sci. USA 90: 4087-4091.
    McLaughlin, S. P., 1986, Differentiation among population of tetraploid Grindelia Camporum. Amer. J. Bot. 73: 1748-1754.
    Moran, G. F. and W. T. Adams, 1989, Microgeographical patterns of allozyme differentiation in douglas- fir from southwest Oregon. For. Sci. 35: 3-5.
    Muller, G., 1977, Cross-fertilization in a conifer stand inferred from enzyme gene-marker in seeds. Silv. Genetica 26: 223-226.
    Nei, M., 1987, Molecular Evolution Genetics. Columbia University Press, New York, USA.
    Nei, M. and F. Tajima, 1981, DNA polymorphism detectable by restriction endonucleases. Genetics 97: 145-163.
    Newell, S. J., 1983, Reproduction in a natural population of cycads (Zamia pumila L.) in Puerto Rico. Bull. Torrey Bot. Club 110: 464-473.
    Niklas, K. J. and K. Norstog, 1984, Aerodynamics and pollen grain depositional patterns on cycad megastrobili: Implications on the reproduction of three cycad genera (Cycas, Dioon, and Zamia). Bot.Gaz. 145: 92-104.
    Norstog, K., 1972, Role of archegonial neck cells of Zamia and other cycads. Phytomorphology 22: 125-130.
    Norstog k. J., 1987, Cycads and the origin of insect pollination. Amer. Sci. 75: 270-279.
    Norstog K. J., P. K. S. Fawcett, 1989, Insect-cycad symbiosis and its relation to the pollination of Zamia furfuracea (Zamiaceae) by Rhopalotria mollis
    (Curculionidae). Amer. J. Bot.76: 1380-1394.
    Norstog, K. J. and T. J. Nicholls, 1997, The Biology of the Cycads, Cornell Univ. Press, Ithaca and London.
    Ornduff, R., 1990, Geographic variation in reproductive behavior and size structure of the Australian cycad Macrozamia communis (Zamiaceae). Amer. J. Bot. 77: 92-99.
    Ornduff, R., 1991, Size classes, reproductive behavior, and insect associates of Cycas media (Cycadaceae) in Australia. Bot.Gaz.152: 203-207.
    Ornduff, R., 1992, Features of coning and foliar phenology, size classes, and insect associations of Cycas armstongii (Cycadeae) in the Northern Territory, Australia. Bull.Terr.Bot. Club 119: 39-43.
    Ornduff, R. 1985, Male-biased sex rations in the cycad Macrozamia riedlei (Zamiaceae). Bull. Torrey Bot. Club 112: 393 - 397.
    Ornduff, R., 1987, Sex ratios and coning frequency of the cycad Zamia pumila L. (Zamiaceae) in the Dominican Republic. Biotropica 19: 361-364.
    Palmer, J. D., R. K. Jansen, H. J. Michaels, M. W. Chase and J. R. Manhart, 1988, Chloroplast DNA variation and plant phylogeny. Ann. Miss. Bot. Gard. 75: 1180-1206.
    Pritchard J. K. and Stephen W. S., 1997, Polymorphism and divergence at a Drosophila pseudogene locus. Genetics 147: 199-208.
    Quijada, A., A. Liston, P. Delgado, A. Vazquez-Lobo and E. R. Alvarz-Buylla, 1998, Variation in the nuclear ribosomal DNA internal transcribed spacer (ITS) region of Pinus rzedowskii revealed by PCR-RELP. Theor. Appl. Genet. 96: 539-544.
    Rathgeber, J. and I. Capesius, 1989, Nucleotide sequence of the 18S-25S spacer region from mustard DNA. Nucleic Acids Res. 17: 7522-7522.
    Ritland, C. and N. A. Straus, 1993, High evolutionary divergence of the 5.8S ribosomal DNA in Mimulus glaucescens (Scrophulariaceae). Plant Mol. Biol. 22: 691-696.
    Rozas, J. and R. Rozas, 1997, DnaSP version 2.0: a novel soft ware package for extensive molecular population genetics analysis. Comput. Applic. Biosci. 13: 307-311.
    Saitou, U. and M. Nei, 1987, The neighbor-joining methods: A new method for reconstructing phylogenetics tree. Mol. Evol. Biol. 4: 406-425.
    Schaal, B. A., 1975, Population structure and local differentiation in Liastris cylindracea. Amer. Nature 109: 511-528.
    Schaal, B. A., 1980, Measurement of gene flow in Lupinus texensis. Nature 284: 450-451.
    Schaal, B. A. and G. H. Learn, 1988, Ribosomal DNA variation within and among plant populations. Ann. Miss. Bot. Gard. 75: 1207-1216.
    Schemske, D. W., 1984, Population structure and local selection in Impatiens (Balsaminaceae), a selfing annual. Evolution 38: 817-832.
    Shen, C. F., K. D. Hill , C. J. Chen, 1994, Cycas taitungensis C. F. Shen K. D. Hill, C. H. Tsou & C. J. Chen, sp. nov. (Cycadaceae), a new name for the widely know cycad species endemic in Taiwan. Bot. Bull. Acad. Sin. 35: 133-140.
    Slatkin, M., 1981., Estimating levels of gene flow in natural populations. Genetics 99: 323-325.
    Slatkin, M., 1985, Gene flow in nature populations. Ann. Rev. Ecol. Syst. 16: 393-430.
    Slatkin, M., 1987, Gene flow and the geographic structure of nature population. Science. 236: 787-792.
    Swofford, D. L., 1993, PAUP: Phylogenetic Analysis Using Parsimony, Version 3.1.1. Computer program distributed by the Illinois Natural History Survey, Champaign, Illinois.
    Smith, D. E. and A. S. Klein, 1994, Phylogenetic inference on the relationship of north american and european Picea based on nuclear ribosomal 18S sequences and the internal transcribed spacer1 region. Mol. Phylogenet. Evol. 3 (1): 17-26
    Soltis, D. E., P. S. Soltis, T. A. Ranker and B. D. Ness, 1988, Chloroplast DNA variation in a wild plant. Genetics 121: 819-826.
    Solits, P. S. and R. K. Kuzoff, 1993, Its sequence variation within and among populations of Lomatium grayi and L. laevigatum (Umbelliferae). Mol. Phy. And Evo. 2 (2): 166-170.
    Steyn, E. M. A. and D. J. F. Strydom, 1993, The nucellus: Its position and function in the ovule of Encephalartos. Encephalartos 35: 14-18.
    Slatkin, M., 1985, Gene flow in natural populations. Genetics. 99: 323-325.
    Taira, T., A. Kato and S. Tanifuji, 1988, Difference between two major size classes of carrot rDNA repeating units is due to reiteration of sequences of about 460bp in the large spacer. Mol. Gen. Genet. 213: 170-174.
    Tajima, F., 1989, Statistical method for testing the neural mutation hypothesis by DNA polymorphism. Genetics 123: 585-595.
    Takaiwa, F., S. Kikuchi, K. Oono and M. Sugiura, 1985, Nucleotide sequence of the 17-25S spacer region from rice rDNA. Plant Moil. Biol. 4: 355-364.
    Tang, -W., 1987, Heat production in cycad cones. Bot-Gaz. 148 (2): 165-174.
    Tang, W., 1989, Seed dispersal in the cycad Zania pumila in Florida. Canaduan. J. Bot. 67: 2066-2070.
    Tsukada M, 1966, Late Pleistocene vegetation and climate in Taiwan (Formosa). Anthropology 55: 543-548.
    Veldman, G. M. J. Klootwyk, H. van Heerikhuizen and R. J. Planta, 1980, some characters of processing sites in ribosomal precursor RNA of yeast. Nucleic Acids Res. 8: 2907-2920.
    Venkateswarlu, K. and R. Nazar, 1991, A conserved core structure in the 18S-25S rRNA intergenic region from tobacco, Nicotiana rustica. Plant Mol. Biol. 17: 189-194.
    Vilgalys, R. and D. Gonzalea, 1990, Ribosomal DNA restriction fragment length polymorphism in Rhizoctonia solani. Phytopathology. 80: 151-158.
    Vorster, P., 1995, Comments on Cycas revoluta. Encephalartos no.2 (March): 1-11.
    Vovides, A. P., 1990, Spatial distribution, survival and fecudity of Dioon edule (Zamiaceae)in a tropical deciduous forest in Veracruz, Mexico, with note on its habitat. Amer. J. Bot. 77: 1532-1543.
    Waldron, J., P. Dunsmuir and J. Bedbrook, 1983, Characterization of the rDNA repeat units in the Mitchell Petunia genome. Plant Mol. Biol. 2: 57-65.
    Walters, T. W. and D. S. Decker- Walter, 1991, Patterns of allozyme diversity in the west indies cycad Zamia pumila (zamiaceae). Amer. J. Bot. 78 (3): 436-445.
    Watkinson, A. R. and J. C. Powell, 1997, The life history and population structure of Cycas armstrongii in monsoonal northern Australia. Oecologia 111:341-349.
    White, T. J., T. Bruns, S. Lee and J. Taylor, 1990, Amplication and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols: A guide to methods and applications, M. Innis, D. Gelfand, J. Sninsky, and T. white (eds), pp. 315-322. Academic Press, San Diego.
    Willams, P. L. and W. M. Fitch, 1989, Finding the minimalchang in a given tree. In Fernholm (eds.), The hierachy of life. pp.453-469. Elsevier Science Publishers B. V.
    Wolfe, K. H., W. H. Li and P. M. Sharp, 1987, Rates of nucleotide substitution vary greatly among plant mitochondrial, chroloplast and nuclear DNAs.Proc. Natl. Acad.Sci. USA 84: 9054-9058.
    Wright, S., 1943a, Isolation by distance. Genetics 28: 114-138.
    Wright, S., 1943b, Analysis of local variablity of flower color in Linanthus parryae. Genetics 28: 139-156.
    Wright, S., 1946, Isolation by distance under diverse systems of mating. Genetics 31: 39-59.
    Wright, S., 1965, The interpretation of population structure by F-statistics with special regard to systems of mating. Evolution 19: 395-420.
    Yang, S. L. and A. W. Meerow, 1996, The Cycas pectinata (Cycadceae) complex: genetic structure and gene flow. Int. J. Sci. 157 (4): 468-483.
    Zhang, Q., M. A. Saghai Maroof and A. Kleinhofs, 1993, Comparative diversity analysis and isozymes within and among population of Hordeum vulgare ssp. spontaneum. Genetics 134: 909-916.

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