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研究生： 徐子欣
Hsu, Tzu-Hsin
論文名稱： 微型氣相層析儀應用於呼氣丙酮及中草藥材氣味分析之研究
The Applications of Micro-GC on Breath Acetone and Herbal Medicine Scent Analysis
指導教授： 呂家榮
Lu, Chia-Jung
口試委員： 周芳妃 宋蕙伶
口試日期： 2021/05/26
學位類別： 碩士
Master
系所名稱： 化學系
Department of Chemistry
論文出版年： 2021
畢業學年度： 109
語文別： 中文
論文頁數： 96
中文關鍵詞： 呼氣丙酮 、非侵入式檢測 、中草藥材氣味 、微型氣相層析儀
英文關鍵詞： breath acetone, non-invasive, Micro-GC, scent of herbal medicine
研究方法: 實驗設計法
DOI URL： http://doi.org/10.6345/NTNU202100747
論文種類： 學術論文
相關次數： 點閱：132  下載：4 
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    • 糖尿病是 全世界 近幾年來非常盛行的慢性文明病，目前也有學者正研究人體呼出的有機揮發性氣 體 (VOCs)所帶給人體生理上的反應，以及利用非侵入式的方法檢測人體 。 另外， 近年來中草藥材中所散發出的氣味 是否正確 也成為人們生活上的一項課題，相同的中草 藥材卻散發出不同的 香氣 ，目前有許多研究與電子鼻相關，模擬了人體的嗅覺模式去進行不同物種的比對。本論文主要以人體呼出 的 有機揮發物以及中草藥材 氣味化合物這兩項為研究主題，人體呼氣的受試者招募了 共 12位 糖尿病患者以及 生理健康人體， 利用本實驗室自製的 氣體採集袋 進行呼氣檢測，而中藥材氣味找尋了杜仲的傳統中草藥材和科學中藥，模擬頂空 的方式採集中草藥材上方的氣體進行研究利用氣相層析質譜儀和本實驗室開發的微型氣相層析儀 進行分析 。本論文研究出了糖尿病患者與呼氣丙酮濃度的相關，以及人體呼氣出的有機揮發物 反應了人體一定的代謝狀態，也探討相同中草藥材於不同產地採收所散發出氣味化合物的異同，和複方科學中藥透過單方科學中藥混合時，所散發出的氣味和濃度是否有加成性 ，並以微型氣相層析儀取代電子鼻，使數據有更高的精確度。

    Diabetes is a chronic disease that is very prevalent all over the world in recent years. At present, some scholars are studying the physiological response of the human body's exhaled Organic Volatile Compounds (VOCs), and the use of non-invasive methods to detect the human body's breath. In addition, in recent years, whether the smell emitted by herbal medicine is correct has also become a topic in our life. The same herbal medicine emits different aromas. At present, there are many studies related to Electronic-noses that simulate the human body’s olfactory pattern to perform different scents and comparison of species.
    The thesis mainly focuses on the two research topics of human body exhaled Organic Volatiles Compounds and herbal medicine compounds. The human exhalation subjects recruited a total of 12 diabetic patients and physically healthy human bodies, and used the self-made gas collection bag in our laboratory to exhale. Detection the smell of herbal medicine searched for herbal medicine materials and scientific herbal medicine of Eucommia ulmoides. The gas above the herbal medicine materials was collected by Headspace (HS). The gas chromatography mass spectrometry and the micro-gas chromatography developed in our laboratory were used for analysis.

    目錄 摘要 I Abstract II 目錄 III 表目錄 VI 圖目錄 VII 第一章 緒論 1 1.1 前言 1 1.2 人體呼氣作用和其所在環境 5 1.3 呼氣中有機揮發物反應之疾病 7 1.3.1 糖尿病與丙酮 7 1.3.2 膽固醇與異戊二烯 9 1.4 電子鼻應用 11 1.5 中草藥氣味 13 1.5.1 杜仲 14 1.5.2 三黃瀉心湯 15 1.5.2.1大黃 16 1.5.2.2黃連 16 1.5.2.3黃芩 17 1.6 人體呼氣揮發性有機物採集方式 18 1.7 中草藥材氣味分析採集方式 19 第二章 實驗部分 20 2.1 實驗藥品與器材 20 2.1.1 實驗藥品 20 2.1.2 實驗儀器與器材 22 2.2 前濃縮系統 23 2.2.1 前濃縮系統介紹 23 2.2.2 六向閥狀態 24 2.2.3 前濃縮管系統與使用流程 27 2.3 氣相層析質譜儀 31 2.4 微型氣相層析儀 32 2.5 樣品採樣方式 33 2.5.1 呼氣中揮發性有機物 33 2.5.2 中草藥材氣味化合物 35 第三章 結果與討論 37 3.1 採樣參數 37 3.1.1 Tedlar採氣袋與自製採氣袋 37 3.1.2 採氣袋回收率 39 3.1.3 氣體來源 42 3.2 呼氣揮發性有機物 42 3.2.1 呼氣中的丙酮 44 3.2.2 呼氣中的異戊二烯 45 3.3 單方中藥材-杜仲 47 3.3.1 台北市與台中市某中草藥材行 49 3.3.2 三間不同廠牌之杜仲科學中藥 60 3.4 複方中藥材-三黃瀉心湯 67 3.5 微型氣相層析儀應用於中草藥材氣味 70 3.5.1 台北市與台中市某中草藥材行 70 3.5.2 三間不同廠牌之杜仲科學中藥 73 3.5.3 複方中藥材-三黃瀉心湯 75 第四章 結論 77 附錄 80 參考文獻 91

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