本研究以氧化石墨烯(Graphene oxide, GO)薄膜覆蓋可撓性的聚醯亞胺(Polyimide, PI)基板，利用超快雷射(Ultrafast laser)產生還原氧化石墨烯(Reduced graphene oxide, rGO)，製作環形指叉狀電極，上層感測區，覆蓋聚丙烯腈(Polyacrylonitrile, PAN)/聚苯胺(Polyaniline, PANI)之靜電紡絲，以製成pH酸鹼感測元件。本研究測試雷射不同的重疊率與單發能量，對應所產生rGO之寬度關係及量測產生rGO的導電性，選出最適當的加工參數。本研究最終選用掃描速度500 mm/s、雷射脈衝頻率300 kHz和能量密度為0.19 J/cm2，在雷射路徑一次製程，製作電極元件，其最低的電阻值為3230 Ω。在材料性質分析下，顯示雷射的能量能有效將GO的含氧官能基去除，還原成rGO。在電紡絲製程方面，電紡絲溶液會以PAN濃度為7 wt%和PANI濃度為1.4 wt%調配比例，在25000 V操作電壓下，產生的電紡絲平滑無珠鏈狀，平均線徑為498.36 nm，該結果經化學分析，證實可將PANI摻入電紡絲中。本研究在製作的設計檢測元件，量測電性響應於不同酸鹼值(pH = 2; 4; 6; 8; 10)，顯示檢測溶液越呈酸性，且其瞬間電流響應越高。另外，在元件靈敏度(電阻變化量/初始電阻)對應pH值反應，顯示每pH量測值會下降6.37 %之線性關係，該其響應結果，可應用於傷口檢測。本研究實際檢測應用，是採以大鼠之傷口為目標檢體，量測3種處理傷口含原始、敷料1(含殼聚糖+奈米金粒子30 μM)及敷料2(含殼聚糖+奈米金粒子60 μM)，分別量測第1天、第4天與第7天之電性變化，顯示在敷料2的傷口檢體pH值，會隨時間變化最小，有助於傷口復原。

In this study, the ultrafast laser can induce the graphene oxide (GO) film into reduce graphene oxide (rGO) and form the circle interdigital electrode patterns on flexible polyimide (PI) substrate. Then, the formed polyacrylonitrile (PAN)/ polyaniline (PANI) electrospinning fibers were on the device of sensing region in detecting the hydrogen ion. To require processing parameters, the different overlap ratios and single plot energy of the laser can be tested to the width of the rGO and the conductivity. In this laser process technique, the scanning speed was 500 mm/s, the laser pulse frequency was 300 kHz, the energy density was 0.19 J/cm2, and the one-step fabrication in laser path was forming electrode device where the rGO device can be shown the lowest resistance value of 3230 Ω. Based on the material measurement, the laser energy can effectively remove the oxygen bonding on GO, showing the reduction of the GO to rGO. In the electrospinning process, the ratio of the electrospinning solution for PAN and PANI can be 7 wt% and 1.4 wt%, respectively. Under the operating voltage of 25000 V, the results of electrospinning filament indicated the smooth and bead-free with an average wire diameter of 498.36 nm. The material analysis demonstrated that PANI was incorporated into electrospinning fibers. The design of fabricating the device can measure the electrical response at the different testing samples, in which pH values were 2, 4, 6, 8, and 10, respectively. At the constant voltage of 1V, the current response to time was suitable to the acid solution when the higher the instantaneous current response was used. The sensitivity corresponds to the pH values, indicating a linear response of decreasing 6.37% per pH value for being applied in wound monitoring. For the practical detection in wound sensing applications, the wound of the rat can be used as the target specimen to measure three kinds of testing wounds, including original, dressing 1 (chitosan + Au nanoparticles 30 μM) and dressing 2 (chitosan + Au nanoparticles 60 μM) to measure the electrical response on day 1, day 4, and day 7 respectively. It revealed that the pH value of dressing 2 was the smallest change with the time, which is helpful for wound healing.

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