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研究生: 林雅芳
Ya-fang Lin
論文名稱: 以形態及分子證據探討玉山蠅子草種複合群的分類問題
Investigations of Taxonomic Problems of Silene morrisonmontana Species Complex Based on Morphological and Molecular Evidences
指導教授: 王震哲
Wang, Jenn-Che
學位類別: 碩士
Master
系所名稱: 生命科學系
Department of Life Science
論文出版年: 2006
畢業學年度: 94
語文別: 中文
論文頁數: 49
中文關鍵詞: 玉山蠅子草種複合群分類基因型
英文關鍵詞: Silene morrisonmontana, species complex, taxonomy, haplotype
論文種類: 學術論文
相關次數: 點閱:481下載:36
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  • 玉山蠅子草種複合群 (Silene morrisonmontana species complex) 包含玉山蠅子草 (Silene morrisonmontana)、禿玉山蠅子草 (S. morrisonmontana var. glabella) 及蓬萊蠅子草 (S. nubigena) 等三個類群,皆為臺灣特有,分布於三千公尺左右的高山地區。本研究以形質分析及分子標記對此一種複合群進行深入研究,形質分析採用12個形態特徵及2個形態比例,對123標本進行形態量化,藉由多變量分析結果推論雪山圈谷之疑問種應歸入蓬萊蠅子草。此種複合群內以植株的被毛程度、萼筒長及雌雄蕊柄長為主要的區分特徵。而分子資料則顯示此種複合群其nrDNA的ITS序列完全一致,兩葉綠體基因片段 (psbA-trnH、rps16) 累積的變異僅10個位點,各類群間基因分化的程度遠少於形態資料所預期,此種複合群應屬形態變異較為快速的物種。因在不同的類群間發現相同的基因型,推論此種複合群應為近年才開始產生基因型分化的物種。

    Silene morrisonmontana species complex consists of three taxa, namely S. morrisonmontana, S. morrisonmontana var. glabella, and S. nubigena. All of them are endemic in alpine regions above 3000 m in elevation of Taiwan. There were controversies over the taxonomic status of members within this species complex. This study aims to investigate the taxonomy of this species complex using morphometric and molecular informations. Twelve morphological characters and two ratio values were quantified for 123 specimens. By applying multivariate analyses, we found that the questionable form in the Hsuehshan cirque should belong to S. nubigena. The main diagnostic characters for this complex include the degree of hair coverage, length of calyx, and length of androgynophore. Molecular data shows that their ITS (nrDNA) sequences are identical from one another, and only 10 variable sites found in selected chloroplast DNA region (psbA-trnH, rps16). Differentiations among haplotypes are low, and incongruent with their diverse morphology. As similar haplotypes were found in different taxa, we infer that this complex must be still in the process of incomplete lineage sorting, and at initial stage in their differentiation after speciation.

    附表目次 II 附圖目次 III 中文摘要 IV 英文摘要 V 壹、前言 1 貳、研究材料與方法 9 參、結果 17 肆、討論 36 伍、參考文獻 41 附錄一 46 附錄二 49 附表目次 表一、DNA抽取樣本及其編號 12 表二、各DNA片段反應條件 14 表三、玉山蠅子草種複合群形質分析各特徵之變方分析表 17 表四、玉山蠅子草種複合群形質分析各特徵之平均值檢測 18 表五、主成分分析各變數之特徵向量及各軸累積所解釋的變異量 22 表六、區別分析所能區別的樣本比例 24 表七、玉山蠅子草種複合群各特徵在區別分析中之特徵向量 25 表八、玉山蠅子草種複合群區別分析之錯分個體統計表 26 表九、A-O基因型在各族群中分布型式 30 表十、玉山蠅子草種複合群內主要區別特徵 38 附圖目次 圖一、玉山蠅子草種複合群形質分析之主成分分析座標圖 23 圖二、玉山蠅子草種複合群形質分析之區別分析座標圖 26 圖三、玉山蠅子草種複合群形質分析之歸群樹形圖 28 圖四、基因型在各族群內的比例圖 31 圖五、基因型最小關聯網狀樹狀圖 32 圖六、合併psbA-trnH、rps16序列,以15個不同的基因型所建構之maximum parsimony tree 34 圖七、合併psbA-trnH、rps16序列,以15個不同的基因型所建構之neighbor joining tree 35

    李祖文 2004 臺灣產石竹科石竹亞科之分類研究。國立臺灣師範大學生命科學系碩士論文。
    趙民德,謝邦昌 2000 多變量分析。曉園出版社有限公司。
    Bernardos, S., A. Crespí, F. D. Rey, and F. Amich. 2005. The section Pseudophrys (Ophrys, Orchidaceae) in the Iberian Peninsula: a morphometric and molecular analysis. Bot. J. Linn. 148: 359-375.
    Choi, K., J. S. Kim, and J.-H. Pak. 2001. Natural hybridization between Pseudostellaria davidii and Pseudostellaria palibiniana (Caryophyllaceae). Pl. Spec. Biol. 16: 39-47. Excoffier, L., and P. E. Smouse. 1994. Using allele frequencies and geographic subdivision to reconstruct gene tree within a species: Molecular variance parsimony. Genetics 136: 343-359.
    Fjellheim, S., R. Elven, and C. Brochmann. 2001. Molecules and morphology in concert. II. The Festuca brachyphylla complex (Poaceae) in Svalbard. Am. J. Bot. 88(5): 869-882.
    Fowler, J., L. Cohen, and P. Jarvis. 1998. Practical statistics for field biology, 2nd ed. Open University Press. pp. 210-234.
    Hansen, K. T., R. Elven, and C. Brochmann. 2000. Molecules and morphology in concert: test of some hypotheses in arctic Potentilla (Rosaceae). Am. J. Bot. 87(10): 1466-1479.
    Hayata, B. 1913. Icones Plantarum Formosanarum. Vol. 3. pp. 38-40. Bureau of Forestry. Taihoku (Taipei), Taiwan.
    Ingvarsson, P. K., S. Ribstein, and D. R. Taylor. 2003. Molecular evolution of insertions and deletion in the chloroplast genome of Silene. Mol. Biol. Evol. 20(11): 1737-1740.
    Jensen, R. J. 2003. The conundrum of morphometrics. Taxon 52: 663-671.
    Kudo, Y. and G. Masamune. 1932. Genera Plantarum Formosanarum. Ann. Rep. Taihoku Bot. Gard. 2: 66-68.
    Kumar, S., K. Tamura, I. B. Jakobsen, and M. Nei. 2001. MEGA2: Molecular Evolutionary Genetics Analysis. Arizona State University, Tempe, Arizona, USA.
    Liu, T. S. and S.-S. Ying. 1976. Silene. In H. L. Li et al. (eds.), Fl. Taiwan. Vol. 2:364-371.
    Marhold, K., J. Lihová, M. Perný, R. Grupe, and B. Neuffer. 2002. Natural hybridization in Cardamine (Brassicaceae) in the Pyrenees: evidence from morphological and molecular data. Biol. J. Linn. Soc. 139: 275-294.
    Masamune, G. 1936. Caryophyllaceae. Short flora of Formosa. pp. 60-62. The editorial department of Kudoa Taihoku, Taiwan.
    Masamune, G. 1954. Caryophyllaceae. A list of vascular plants of Taiwan. pp. 43-44.
    McCauley, D. E. 1994. Contrasting the distribution of chloroplast DNA and allozyme polymorphism among local populations of Silene alba: implications for studies of gene flow in plants. Proc. Natl. Acad. Sci. 91: 8127-8131.
    Möller, M., and Q. C. B. Cronk. 1997. Origin and relationships of Saintpaulia (Gesneriaceae) based on ribosomal DNA internal transcribed spacer (ITS) sequences. Am. J. Bot. 84(8): 956-965.
    Nielsen, L. R., R. S. Cowan, H. R. Siegismund, H. Adsersen, M. Philipp, and M. F. Fay. 2003. Morphometric, AFLP and plastid microsatellite variation in populations of Scalesia divisa and S. incise (Asteraceae) from the Galápagos Islands. Biol. J. Linn. Soc. 143: 243-254.
    Ohwi, J. 1934. Plantae Novae Japonicae. Fedde Repert. 36: 47
    Ohwi, J. 1943. Symbolae ad Floram Asia Orientalis XIX. Acta Phytotax. Geobot. 12:113.
    Ohwi, J. and H. Ohashi. 1974. New combination in Japanese and Formosan Silene (Caryophyllaceae). J. Jap. Bot. 49: 345.
    Olson, M. S. and D. E. McCauley. 2000. Linkage disequilibrium and phylogenetic congruence between chloroplast and mitochondrial haplotypes in Silene vulgaris. Proc. R. Lond. (Roy). 267: 1801-1808.
    Oxelman, B. and M. Lidén. 1995. Generic boundaries in the tribe Sileneae (Caryophyllaceae) as inferred from nuclear rDNA sequences. Taxon 44: 525-542.
    Oxelman, B., M. Lidén, and D. Berglund. 1997. Chloroplast rps16 intron phylogeny of the tribe Sileneae (Caryophyllaceae). Pl. Syst. Evol. 206: 393-410.
    Popp, M. and B. Oxelman. 2001. Inferring the history of the polyploid Silene aegaea (Caryophyllaceae) using plastid and homoeologous nuclear DNA sequences. Mol. Phyl. Evol. 20:474-481.
    Popp, M., P. Erixon, F. Eggens, and B. Oxelman. 2005. Origin and evolution of a circumpolar polyploid species complex in Silene (Caryophyllaceae) inferred from low copy nuclear RNA polymerase introns, rDNA, and chloroplast DNA. Syst. Bot. 30(2): 302-313.
    Puorto, G., M. D. G. Salomão, R. D. G. Theakston, R. S. Thorpe, D. A. Warrell, and W. Wüster. 2001. Combining mitochondrial DNA sequences and morphological data to infer species boundaries: phylogeography of lanceheaded of Bothrops pradoi (Squamata: Serpentes: Viperidae). J. Evol. Biol. 14: 527-538.
    Quiroga, M. P., A. C. Premoli, and C. Ezcurra. 2002. Morphological and isozyme variation in Cerastium arvense (Caryophyllaceae) in the southern Andes. Can. J. Bot. 80: 786-795.
    Rozas, J., J. C. Sanchez-Delbarrio, X. Messeguer and R. Rozas. 2003. DnaSP, DNA polymorphism analyses by the coalescent and other methods. Bioinformatics 19: 2496-2497.
    Sharbel, T. F. and T. Mitchell-olds. 2001. Recurrent polyploidy origins and chloroplast phylogeography in the Arabis holboellii complex (Brassicaceae). Heredity 87:59-68.
    Shipunov, A. B., M. F. Fay, and M. W. Chase. 2005. Evolution of Dactylorhiza baltica (Orchidaceae) in European Russia: evidence from molecular markers and morphology. Biol. J. Linn. Soc. 147: 257-274.
    Soliva, M., A. Kocyan, and A. Widmer. 2001. Molecular phylogenetics of the sexually deceptive orchid genus Ophrys (Orchidaceae) based on nuclear and chloroplast DNA sequences. Mol. Phyl. Evol. 20: 77-88.
    Swofford, D. 2000. PAUP*. Phylogenetic analysis using parsimony (*and other methods). Version 4. Sunderland, Massachusetts: Sinauer Associates.
    Tang, C. L. 1996. Silene. In Flora Reipublicae Popularis Sinicae. Tomus. 26. pp 278-402. Science Press. Beijing.
    Whang, S. S., K. Choi, R. S. Hill, and J. H. Pak. 2002. A morphometric analysis of intraspecific taxa within the Ixeris chinensis complex (Asteraceae, Lactuceae). Bot. Bull. Acad. Sin. 43:131-138.
    Ying, S.-S. 1975a. A list of alpine plants of Taiwan. Quart. J. Chin. For. 8(3): 119-120.
    Ying, S.-S. 1975b. Alpine plants of Taiwan in color. vol. 1. pp. 57-58. Published by the auther. Taipei.
    Ying, S.-S. 1984. Coloured illustrated flora of Taiwan. Vol. 1. p. 694. Published by the author. Taipei.
    Ying, S-S. 1996. Caryophyllaceae. In T. C. Huang et al. (eds.), Flora of Taiwan, 2nd ed. 2: 364-371.
    Zhou, L.-H., Wu Z.-Y., M. Lidén, and B. Oxelman. 2001. Silene. In Z.-Y. Wu & P. H. Raven (eds.), Flora of China. Vol 6. pp. 81-82. Science Press.

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