本研究之目的在開發吳茱萸藥材、吳茱萸黃連藥對及其相關方劑的高效液
相層析法。吳茱萸藥材中富含生物鹼，本實驗以醋酸/醋酸鈉的緩衝液為
沖提系統，於 250 nm 的偵測波長及線性梯度沖提程式下，在 45 min 內
完成吳茱萸藥材中十一個三級生物鹼和一個四級生物鹼的定量工作。此外
，分離管柱之選擇、移動相添加物對吳茱萸藥材各成分層析效果的影響及
移動相中有機修飾劑的選擇在文中均有詳細的探討。黃連藥材中含有許多
的四級生物鹼，本研究以吳茱萸黃連藥對作為分析條件開發的對象。於實
驗中我們開發了兩種沖提系統來定量藥對中七個四級生物鹼和十個三級生
物鹼。一是以 NaOAc 為離子對試劑的沖提系統，另一則是以 SDS 為主要
離子對試劑的沖提系統。本文中除了對兩沖提系統之離子對試劑的濃度、
不同有機修飾劑的比例、不同型態的前置管柱及注射前之平衡時間等變因
進行探討外，亦比較兩沖提系統之優劣。最後選擇以 NaOAc 為離子對試
劑的沖提系統，在 65 min 內，於 250 nm 的偵測波長及線性梯度沖提程
式下，定量分析吳茱萸黃連藥對中十七個生物鹼成分，並將此方法運用於
藥對相關製劑變通丸之定量。疏肝湯為含有吳茱萸黃連藥對之方劑，由十
種藥材所組成。本實驗結合分析吳茱萸黃連藥對之醋酸/醋酸鈉沖提系統
，及新開發的磷酸/磷酸鉀沖提系統來分析此方劑，共計可定量疏肝湯中
二十四個成分。

A high-performance liquid chromatographic method forsimultaneous
determination of eleven tertiary alkaloids and onequarternary
alkaloid in Evodiae Fructus was developed. Themethod was carried
out by using a gradient solvent system ofacetate buffer-
acetonitrile-methanol and detecting at 250 nm , andthe contents
of the alkaloids in a non-pretreatment EvodiaeFructus extract
could easily be determined within 45 nim. Theeffect of column
selectivity, additive concentration and organicmoeifier
concentration of the mobile phase on the retention andseparation
of the solutes were also studied. Two liquid chromatographic
methods with linear gradient solventsystems were developed to
determine the seven quarternaryalkaloids and ten tertiary
alkaloids in coptis-evodia herb couple.In those two methods, one
used NaOAc as the ion-pair reagent,and the other used SDS as the
main ion-pair reagent. The effecton the concentration of ion-
pair reagent, the ratio of organicmodifiers, the types of
precolumn and the balance time beforeevery injection of the two
gradient solvent systems to theretention and separation of the
solutes were discussed in detail.After comparing the two solvent
systems, we selected the acetatebuffer solution as eluent and
detected at 250 nm for determinatingthe seventeen alkaloids in
coptis-evodia herb couple within 65min. This method was also
applied to analyse the formulas Pien-tung-wan.Shu-kan-tang, a
Chinese herbal preparation, is known to consistof ten herb
drugs, including the coptis-evodia herb couple. 13compounds of
its 24 components can be determined by themethod for analysing
coptis-evodia herb couple, and 18compounds of its 24 components
can be assayed by a LC methodusing gradient solvent system that
contained phosphate buffer.
A high-performance liquid chromatographic method forsimultaneous