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研究生：	張嘉芸 Chia-Yun Chang
論文名稱：	阿達瑪轉換/毛細管電泳法適用之奈米流量進樣器的開發與研究 Development of a nano-controlled sample injection device for use in Hadamard transform/capillary electrophoresis (HT/CE)
指導教授：	林震煌 Lin, Cheng-Huang
學位類別：	碩士 Master
系所名稱：	化學系 Department of Chemistry
論文出版年：	2011
畢業學年度：	99
語文別：	中文
論文頁數：	87
中文關鍵詞：	阿達瑪轉換-毛細管電泳法、維他命B2
論文種類：	學術論文
相關次數：	點閱：121 下載：2
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本研究設計一個新的奈米流量（nano-controlled）進樣器，並成功結合阿達瑪轉換法（Hadamard transform）與毛細管電泳法（capillary electrophoresis）。其中，首次利用壓克力Tee型連接頭將進樣器及毛細管電泳裝置連結。
　　實驗過程為將10 μL的注射針置於注射式幫浦（syringe pump）上，再NI（National Instruments）PCI-6221程式控制注射式幫浦內部之步進馬達（stepping motor）轉動步數，進一步控制進樣體積。當設定步進馬達轉動步數為750~20000步（進樣體積為1.3 nL~49.4 nL）的範圍時，具有良好的線性關係（R2 =0.9927）。本研究以核黃素（又稱維他命B2）作為分析物，並以藍光雷射（波長473 nm, 100 mW）作為激發光源。當分析物推入壓克力Tee且經電泳分離後，通過偵測窗時，由雷射激發且放出螢光，最後以光電倍增管（PMT）作偵測。在相同的進樣條件下，以阿達瑪127、255次序列分別進行實驗，訊號雜訊比可得到5.3及7.9倍的改良效果，與理論值（5.6與8）相當符合。最後，本實驗嘗試以阿達瑪127次序列進樣與毛細管線上濃縮技術進行結合，結果顯示其S/N比增加5.2倍，與理論值相當接近。
關鍵字：阿達瑪轉換／毛細管電泳法、維他命B2

A novel nano-controlled sample injection device for use in Hadamard transform/capillary electrophoresis (HT/CE) was successfully developed. Instead of commercial Tee products, an acrylic-Tee connector was well designed and made in-house. Three pieces of capillaries (used for sample solution and buffer solutions, respectively) can be tightly connected together with very low dead-volume. The sample solution was placed in a syringe injector (size, 10 µL) and was pushed out by a stepping motor which was controlled by a personal computer through a NI (National Instruments) PCI-6221 device. The volume of sample injection can be well controlled and calculated based on the steps (750- ~ 20,000-steps) of the stepping motor, corresponding to the injected volume of 1.3 nL ~ 49.4 nL. A riboflavin solution and a blue diode laser (wavelength, 473 nm; 100 mW) were selected as a model compound and the light source, respectively. Compared with a conventional single injection method, the S/N ratios were substantially improved after inverse Hadamard transformation of the encoded chromatogram. Under optimized conditions, when Hadamard matrices of 127 and 255 were used, the S/N ratios of the signals for riboflavin (concentration level, 0.03 ppm) were substantially improved to 5.3- and 7.9-fold, respectively, and those improvements are in good agreement with those obtained by theory (5.6- and 8.0-fold).

中文摘要    Ⅰ
英文摘要    Ⅱ
目錄    Ⅲ
圖目錄    Ⅴ
表目錄    Ⅵ

第一章、緒論    1
1-1    研究目的    1
1-2    分析物簡介    3
1-2-1　維他命B2     3
1-2-2　孔雀石綠染料    6

第二章、分析方法與原理    8
2-1  毛細管電泳層析法之分離模式    8
    2-1-1  毛細管區帶電泳（CZE）    9
    2-1-2  微胞電動層析法（MEKC）    12
2-2   毛細管線上濃縮技術    16
    2-2-1  毛細管電泳線上堆積法（stacking）    18
2-3    阿達瑪矩陣原理    20
    2-3-1  矩陣起源    21
    2-3-2  阿達瑪轉換法    22
    2-3-3  LabVIEW 操作程式    29
    2-3-4  阿達瑪轉換提高S/N值的理論值    31
2-4   阿達瑪矩陣轉換在毛細管電泳上的應用    34

第三章、實驗    35
3-1    壓克力Tee型連接頭的製作    35
3-2    進樣裝置    37
    3-2-1  機械式進樣    37
    3-2-2  氣動式進樣    39
3-3    實驗儀器裝置    42
    3-3-1  自組式毛細管電泳／雷射誘導螢光分析儀（CE- LIF）    42
    3-3-2  自組式毛細管電泳／拉曼分析儀（CE- Raman）    44
3-4    儀器及週邊設備列表    46
3-5    使用藥品列表    50
3-6    氣動式進樣－毛細管電泳／拉曼分析儀之實驗條件    51
    3-6-1  氣動式進樣之條件    51
    3-6-2  毛細管區帶電泳（CZE）電泳條件的確立與配製    51

3-7    機械式進樣－毛細管電泳／雷射誘導螢光儀之實驗條件    52
    3-7-1  毛細管區帶電泳（CZE）電泳條件的確立與配製    52
    3-7-2  線上濃縮技術CZE-stacking條件的確立與配製    53

第四章、結果與討論    54
4-1    市售三向連接頭與壓克力Tee型連接頭之比較    54
4-2    氣動式進樣－毛細管電泳／拉曼分析儀    56
    4-2-1  氣動式進樣－毛細管電泳／拉曼分析儀之電泳圖探討    56
    4-2-2  壓力式進樣的穩定度    61
4-3   機械式進樣－毛細管電泳／雷射誘導螢光分析儀    64
    4-3-1  緩衝溶液的選擇    64
    4-3-2  步進馬達之穩定度    66
    4-3-3  機械進樣之穩定度    68
    4-3-4  以阿達瑪序列進樣及轉換結果與理論值比較    72
    4-3-5  以阿達瑪序列進樣搭配CZE-stacking    76

第五章、結論    83

參考文獻    84
                                

[1] C. T. Culbertson, J. W. Jorgenson, Anal. Chem. 70 (1998) 2629.
[2] J. A. McReynolds, S. A. Shippy, Anal. Chem. 76 (2004) 3214.
[3] H. J. Crabtree, M. U. Kopp, A. Manz, Anal. Chem. 71 (1999) 2130.
[4] Y. C. Kwok, A. Manz, Electrophoresis, 22 (2001) 222.
[5] J. N.van der Moolen, D. J. Louwerse, H. Poppe, H. C. Smit, Chromatographia 40 (1995) 368.
[6] J. C. Fister, S. C. Jacobson, J. M. Ramsey, Anal. Chem. 71 (1999) 4460.
[7] T. Kaneta, Anal. Chem. 73 (2001) 540a.
[8] T. Kaneta, K. Kosai, T. Imasaka, Anal. Chem. 74 (2002) 2257.
[9] T. Kaneta, Y. Yamaguchi, T. Imasaka, Anal. Chem. 71 (1999) 5444.
[10] P. Zeppenfeld, M. Krzyzowski, C. Romainczyk, R. David, Rev. Sci. Instrum. 64 (1993) 1520.
[11] Hanley, Q. S. Appl. Spectrosc. 55 (2001) 318.
[12] K. Hata, Y. Kichise, T. Kaneta, T. Imasaka, Anal. Chem. 75 (2003) 1765.
[13] K. Hata, T. Kaneta, T. Imasaka, Anal. Chem. 76 (2004) 4421.
[14] K. L. Braun, S. Hapuarachchi, F. M. Fernandez, and C. A. Aspinwall, Anal. Chem. 78 (2006) 1628.
[15] D. Yamamoto, T. Kaneta, T. Imasaka, Electrophoresis, 28 (2007) 4143.
[16] P. F. Heelis, in: F. Müller (Ed.), CRC Press, Boca Raton, FL, (1991) 171.
[17] T. R. I. Cataldi, D. Nardiello, G. E. De Benedetto, S. A. Bufo, J. Chromatogr. A, 968 (2002) 229.
[18] F. Valls, M. T. Sancho, M. A. fernández-Muiño, M. A. Checa, J. Agric. Food Chem. 47 (1999) 1067.
[19] C. D. Capo-chichi, J.-L. Guéant, F. Feillet, F. Namour, M. Vidailhet, J. Chromatogr. B 739 (2000) 219.
85
[20] S. Hustad, P. M. Ueland, J. Schneede, Clin. Chem. 45:6 (1999) 862.
[21] T. R. I. Cataldi, D. Nardiello, L. Scrano, A. Scopa, J. Agric. Food Chem. 50 (2002) 6643.
[22] J. H. Wassink, S. G. Mayhew, Anal. Biochem. 68 (1975) 609.
[23] J. A. Tillotson, M. M. Bashor, Anal. Biochem. 107 (1980) 214.
[24] M. G. Duyvis, R. Hilhorst, C. Laane, D. J. Evans, D. J. M. J. Agric. Food Chem. 50 (2002) 1548.
[25] M. M. Dittmann, G. P. Rozing. J. Chromatogr. A, 744 (1996) 63
[26] A. Cohen, B. L. Karger. J. Chromatogr. A, 397 (1987) 409
[27] P. Scherpenisse, A. A. Bergwerff, Anal. Chim. Acta, 529 (2005) 173
[28] M. Mazereeuw, V. Spikmans, U. R. Tjaden, J. Chromatogr. A, 1067 (2005) 101
[29] S. K. Otsuka, K. Ichikawa, A. Tsuchiya, T. Ando, Anal. Chem. 56 (1984) 111
[30] L. G. Rushing, J. Thompson, J. Chromatogr. B, 688 (1997) 325
[31] K. Mitrowska, A. Posyniak, J. Zmudzki, J. Chromatogr. A, 1089 (2005) 187
[32] N. Haagsma, C. A. J. Hajee, J. Chromatogr. B, 669 (1995) 219
[33] J. W. Joegenson, K. D. Lukacs, J. Chromatogr. 218 (1981) 209.
[34] J. W. Joegenson, K. D. Lukacs, Anal. Chem. 53 (1981) 1298.
[35] K. Otsuka, K. Ichikawa, A. Tsuchiya, T. Ando, Anal. Chem. 56 (1984) 111.
[36] S. Terabe, K. Otsuka, T. Ando, Anal. Chem. 57 (1985) 834.
[37] K. H. Row, W. H. Griest, M. P. Maskarienc, J. Chromatogr. 409 (1987) 193.
[38] A. Cohen, B. L. Karger, J. Chromatogr. 397 (1987) 409.
[39] S. Hjerten, M. D. Zhu, J. Chromatogr. 346 (1985) 265.
[40] S. Hjerten, J. L. Liao, K. Yao, J. Chromatogr. 387 (1987) 127.
[41] X. Huang, R. N. Zare, Anal. Chem. 63 (1991) 2193.
[42] R. D. Holland, M. J. Sepaniak, Anal. Chem. 65 (1993) 1140.
[43] X. Huang, M. J. Gordon, R. N. Zare, Anal. Chem. 60 (1993) 375.
86
[44] R. T. Kennedy, J. W. Gorgenson, Anal. Chem. 61 (1989) 1128.
[45] D. N. Heiger, Hewlett-Packard Company Publication Number 12-5091-6199E.
[46] H. Z. Helmholtz, Anal. Phys. Chem. 7 (1897) 337.
[47] B. Krattiger, G. J. M. Bruin, A. E. Bruin, Anal. Chem. 66 (1994) 1.
[48] M. Stefansson, M. Novotny, Anal. Chem. 66 (1994) 1134.
[49] Y. Kim, M. D. Morris, Anal. Chem. 66 (1994) 1168.
[50] Z. Zhzo, A. Malik, M. L. Lee, Anal. Chem. 65 (1994) 2747.
[51] K. Otsuka, K. Ichikawa, A. Tsuchiya, T. Ando, Anal. Chem. 56 (1984) 111.
[52] D. N. Heiger, Hewlett-Packard Company Publication Number 12-5091-6199E.
[53] R.-L. Chien, in: M. G. Khaledi, (Ed.), High Performance Capillary Electrophoresis (Theory, Techniques and Applications), Chapter 13, CRC Press, (1998).
[54] Z. Liu, P. Sam, S. R. Sirimanne, P. C. McClure, J. Grainger, D. G. Patterson, J. Chromatogr. A 673 (1994) 125.
[55] K. R. Nielson, J. P. Foley, J. Chromatogr. A 686 (1994) 283.
[56] J. P. Quirino, S. Terabe, J. Chromatogr. A 781 (1997) 119.
[57] J. P. Quirino, S. Terabe, Science 282 (1998) 465.
[58] C.-X. Zhang, W. Thormann, Anal. Chem. 70 (1998) 540.
[59] Z. K. Shihabi, J. Chromatogr. A 817 (1998) 25.
[60] J. Palmer, N. J. Munro, J. P. Landers, Anal. Chem. 71 (1999) 1679.
[61] J. P. Quirino, S. Terabe, Anal. Chem. 72 (2000) 1023.
[62] P. Britz-McKibbin , A. R. Kranack, A. Paprica, D. D. Y. Chen, Analyst 123 (1998) 1461.
[63] D. S. Burgi, Anal. Chem. 65 (1993) 3726.
[64] J. P. Quirino, S. Terabe, J. Chromatogr. A 850 (1999) 339.
87
[65] Z. K. Shihabi, J. Chromatogr. A 744 (1996) 231.
[66] D. Martínez, F. Borrull, M. Calull, J. Chromatogr. A 788 (1997) 185.
[67] R. Kuldvee, M. Kaljurand, Anal. Chem. 70 (1998) 3695.
[68] Y. He, H.-K. Lee, Anal. Chem. 71 (1999) 995.
[69] J. Palmer, J. P. Landers, Anal. Chem. 72 (2000) 1941.
[70] S. Locke, D. Figeys, Anal. Chem. 72 (2000) 2684.
[71] W.-H. Ding, C.-H. Liu, J. Chromatogr. A 929 (2001) 143.
[72] C.-X. Cao, Y.-Z. He, M. Li, Y.-T. Qian, M.-F. Gao, L.-H. Ge, S.-L. Zhou, L. Yang, Q.-S. Qu, Anal. Chem. 74 (2002) 4167.
[73] Sylvester, J. J. Philosophical Magazine. 34 (1867) 461.
[74] Griffiths, P. R., ed. “Transform Techniques in Chemistry. Modern AnalyticalChemistry Series” Plenum Press: New York, 1978.
[75] Literature Seminar, Pan, C. “Applications of The Hadamard Transform in Analytical Chemistry” 2007, 3rd, pp. 3.
[76] Yates, F. J. Roy. Stat. Soc. Supp. 2 (1935) 181.
[77] Fellgett, P. J. de Physique et le Radium. 19 (1958) 187.
[78] Hotelling, H. Ann. Math. Stat. 15 (1944) 297.
[79] Zupan, J.; Bohanec, S.; Razinger, M.; Novic, M. Analytical Chimica Acta. 210 (1988) 63.
[80] Smit, H. C. Chromatographia 3 (1970) 515.
[81] Kaljurand, M.; Kūllik, E. J. Chromatogr. 171 (1979) 243.
[82] F. Lorraine, O. Darren A., S. W. Franklin, Anal. Chim. Acta. 349 (1997) 221.
[83] S. C. Su, S. S. Chou, D. F. Hwang, P. C. Chang, C. H. Liu, J. Food. Science, 66 (2001) 10.
[84] R. Huopalahti, J. Sunell, Journal of Chromatography, 636 (1993) 133.
[85] L. F. Russell, L. Brooks, K. B. McRae, Food Chem. 63 (1997) 125.

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