本研究提出一個新穎檢測肝癌腫瘤的活體檢驗方法，利用批覆Alphafetaprotein(AFP)抗體的磁性奈米粒子(Magnetic nanoparticles,MNPs)，注射於癌鼠進行標靶實驗，並開發掃描式超導磁導儀(Scanning superconducting-quantum-interference device, SSB)檢驗MNPS的交流磁化率。本研究除了用SSB對注射MNPs試劑的癌鼠進行檢測，也利用MRI與切片染色方法進行驗證，SSB、MRI與切片染色結果的一致性證實了SSB的可行性與MNPs標靶於腫瘤位置的專一性。因此未來病人可注射批覆AFP抗體的磁性奈米粒子試劑後用SSB進行活體篩檢，如需進一步精確斷層掃描則再花較多的MRI診斷費，減少民眾與健保的負擔。許多醫學影像技術如MRI、X-ray，雖具有高解析的斷層影像，但因造價維護費用高且需另有良好的屏蔽環境，因此往往僅大型醫療機構能採購。此外，為增加對腫瘤辨識的靈敏度與專一性，以批覆生物探針的奈米粒子已為主流。

[1]. Hong CY, Wu CC, Yang SY, et al. Magnetic susceptibility reduc-tion method for magnetically labeled immunoassay. Appl Phys Lett.;88:212512-1 ( 2006)
[2]. Huang KW, Yang SY, Hong YW, et al. Feasibility studies for assaying alpha-fetoprotein using antibody- activated magnetic nanoparticles. Int J Nanomed.;7:1991 (2012)
[3]. Rosensweig RE. Heating magnetic fluid with alternating magnetic field. J Magn Magn Mater. ;252:370(2002)
[4]. Tseng HY, Lee GB, Lee CY, et al. Localised heating of tumours utiliz¬ing injectable magnetic nanoparticles for hyperthermia cancer therapy. IET Nanobiotechnol.;3(2):46(2009)
[5]. Duguet E, Vasseur S, Mornet S, Devoisselle JM. Magnetic nanopar¬ticles and their applications in medicine. Nanomedicine.;1(2): 157(2006)
[6]. Prijic S, Sersa G. Magnetic nanoparticles as targeted delivery systems in oncology. Radiol Oncol.;45(1):1(2011)
[7]. Nair BG, Nagaoka Y, Morimoto H, et al. Aptamer conjugated mag¬netic nanoparticles as nanosurgeons. Nanotechnology.;21(45): 455102-1 (2010)
[8]. Moritz FK, Umar M, Raymond SK, et al. A multimodal nanoparticle for preoperative magnetic delineation resonance imaging and intraoperative optical brain tumor. Cancer Res.;63:8122(2003)
[9]. Watkin KL, McDonald MA. Multi-moddal contrast agents:a first step. Academic Radiology 9:S285 (2002)
[10]. Chieh JJ, Hong CY. Non-invasive and high-sensitivity scanning detection of magnetic nanoparticles in animals using high-Tc scanning superconducting-quantum-interference-device biosusceptometry. Rev Sci Instrum.;82(8):084301-1(2011)
[11]. Martin Nikolo.A guide to alternating current susceptibility measurements and alternating current susceptometer design.American Association of Physics Teachers 57-65
[12]. Chieh JJ, Tseng WK, Horng HE, et al. In-vivo and real-time measure¬ment of magnetic-nanoparticles distribution in animals by scanning SQUID biosusceptometry for biomedicine study. IEEE IEEE Trans Biomed Eng.;58(10):2719(2011)
[13]. Tseng WK, Chieh JJ, Yang SY, et al. In-vivo and fast examination of iron concentration of magnetic nano-particles in an animal torso via scanning SQUID Biosusceptometry. IEEE Trans Appl Supercond.;21(3):2250–2253(2011)
[14]. Tadayuki Kondo1 and Hideo Itozaki Normal conducting transfer coil for SQUID NDE SUPERCONDUCTOR SCIENCE AND TECHNOLOGY,17,459(2004)
[15]. Y. S. Greenberg, “Application of superconducting quantum interferencedevices to nuclear magnetic resonance,” Rev. Mod. Phys., vol. 70:175(2002)
[16]. R. McDermott, A. H. Trabesinger, M. Mück, E. L. Haln, A. Pines, and J. Clarke, “Liquid-state NMR and scalar couplings in microtesla magnetic fields ,” Science, vol. 295: 2247 (2002)
[17]. Y. Zhang, L. Qiu, H. Krause, S. Hartiwig, M. Burghoff, and L. Trahms, “Liquid state nuclear magnetic resonance at low fields using a nitrogen cooled superconducting quantum interference device,” Appl. Phys. Lett., 90:182503, (2007)
[18]. K. Schlenga, R. McDermott, J. Clarke, R. E. de Souza, A. Wong-Foy, and A. Pines, “Low-field magnetic resonance imaging with a high- Tc dc superconducting quantum interference device,” Appl. Phys. Lett., 75:3695, (1999)
[19]. M. Burghoff, S. Hartwig, L. Trahms, and J. Bernarding, “Nuclear magnetic resonance in the nanoTesla range,” App. Phys. Lett., 87:054103, 2005
[20]. L. Qiu, Y. Zhang, H. J. Krause, A. H. Braginski, M. Burghoff, and L. Trahms, “Nuclear magnetic resonance in the earth’s magnetic field using a nitrogen-cooled superconducting quantum interference device,” APPLIED PHYSICS LETTERS ,91:072505(2007)
[21]. H. C. Yang, S. H. Liao, H. E. Horng, S. L. Kuo, H. H. Chen, and
S. Y. Yang, “Enhancement of nuclear magnetic resonance in microtesla
magnetic field with prepolarization field detected with high-Tc superconducting quantum interference device,” Appl. Phys. Lett., 88:252505( 2006)
[22]. Hill DA Further studies of human whole-body radiofrequency absorptionrates. Bioelectromagnetics 6: 33(1985)
[23]. Horng HE, Yang SY, Huang YW, Jiang WQ, Hong CY, et al. Nanomagnetic Particles for SQUID-based Magnetically Labeled Immunoassay.IEEE Trans Appl Supercond. 15: 668(2005)
[24]. Yang SY, Jian ZF, Horng HE, Hong CY, Yang HC, et al. Dualimmobilization and magnetic manipulation of magnetic nanoparticles. J MagnMagn Mater,.320: 2688 (2008)
[25]. Chieh JJ, Hong CY Non-invasive and High-sensitivity scanning detection of magnetic nanoparticles in animals using high-Tc scanning superconductingquantum-interference-device biosusceptometry. Rev Sci Instrum .82: 084301-1 (2011)
[26]. Yang SY, Sun JS, Liu CH, et al. Ex vivo magnetofection with magnetic nanoparticles: a novel platform for nonviral tissue engineering. Artif Organs.;32(3):195(2008)
[27]. Wang J, Chen Y, Chen B, Ding J, Xia G, et al. Pharmacokinetic parameters and tissue distribution of magnetic Fe3O4 nanoparticles in mice. International Journal of Nanomedicine5:861(2010)
[28]. Tsuchiya K, Nitta N, Sonoda A, Seko AN, Ohta S, et al. Histological study of the biodynamics of iron oxide nanoparticles with different diameters.Int J Nanomed, 6:1587(2011)
[29]. Huang KW, Yang SY, Hong YW, Chieh JJ, Yang CC, et al. Feasibility studies for assaying alpha-fetoprotein using antibody- activated magnetic nanoparticles. Int. J. Nanomed.7: 1991(2012)
[30]. Yang SY, Jian ZF, Horng HE, et al. Dual immobilization and mag¬netic manipulation of magnetic nanoparticles. J Magn Magn Mater.;320(21):2688( 2008)