本研究以高表面積 (SBET> 900 m2 / g) 的中孔沸石奈米粒子 (mesoporous zeolite nanoparticles, MZNs) 做為基材，於高溫下 (825-950 °C) 和乙烯氣體進行化學氣相沉積 (chemical vapor deposition, CVD) 反應，並以氮氣等溫吸脫附來鑑定石墨烯化且孔徑均勻的中孔氧化石墨烯奈米粒子 (mesoporous graphene-oxide nanoparticles, MGNs)。界達電位分析證實表面的電荷變化並以不同電性的銀前驅物、合成條件來有效調控奈米粒子之形貌，佐以電子顯微鏡、紫外-可見光吸收光譜、X光粉末繞射來鑑定銀奈米粒子顆粒之大小 (寛6-18奈米、長12-33奈米)。受孔洞限制生長的粒子不僅提供表面電漿熱點，其表面拉曼增強 (surface-enhanced Raman spectroscopy，SERS) 效應可偵測到極低濃度 (10-10 M) 的甲基紫10B 染料 (crystal violet, CV)，其穩定性受到氧化石墨烯的作用而提升至七日。目前己成功以三種方式附載於紙基晶片上，未來積極投入濫用藥物結合基質輔助雷射解吸∕電離 (matrix-assisted laser desorption/ionization, MALDI) 雙功能的偵測分析應用。

In this study, mesoporous zeolite nanoparticles (MZNs) with high surface area (SBET> 900 m2/g) were used as the substrates, and chemical vapor deposition (CVD) reaction with ethylene gas was carried out at high temperature (825-950 °C). The isothermal adsorption and desorption of nitrogen was used to identify the mesoporous graphene-oxide nanoparticles (MGNs) with grapheneization and uniform pore size. Zeta potential analysis confirmed the change of surface charge. The silver precursors were introduced with different electrical properties, and conditions of synthesis were effectively controlled the morphology of nanoparticles. Accompanied by electron microscopy, ultraviolet-visible spectroscopy, and X-ray powder diffraction were used to identify the size of silver nanoparticle particles (width 6-18 nm, length 12-33 nm). The growth of particles restricted by pores not only provides surface plasmonic hot spots, but its surface-enhanced Raman spectroscopy (SERS) effect can detect very low concentrations (10-10 M) of methyl violet 10B dye (crystal violet, CV), which could improve stability to seven days by the interaction of graphene oxide. At present, MZNs has been successfully attached to paper-based chips in three ways. In the future, our study will actively be invested in the detection and analysis of drugs of abuse combined with matrix-assisted laser desorption/ ionization (MALDI) dual-function detection and analysis applications.

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