蕹菜（Ipomoea aquatica Forsk.）為台灣重要的夏季蔬菜，卻容易感染白銹病。由子葉感染法感染蕹菜幼苗的結果發現，白籽竹葉蕹菜對於白銹病的抗病能力最強。在可能與抗病相關的蕹菜生理性狀探究中，氣孔密度、光合色素含量（葉綠素a、葉綠素b和類胡蘿蔔素）、以及葡萄糖與果糖濃度對蕹菜的幼苗發病率有非線性正相關，而蔗糖濃度與幼苗發病率成負相關，其中氣孔密度、葉綠素a、葉綠素b、葡萄糖與果糖濃度之XY散佈圖數據呈分群的現象。植株園藝性狀對蕹菜感染白銹病的嚴重程度成複迴歸關係，共有六種園藝性狀參與，此複迴歸方程式對罹病率的預測度可達六成。進一步以逢機引子增殖多型性分子標誌技術(RAPD)分析強抗病品系2002-026與感病品系之間的差異，逢機引子O15在相同PCR條件下，顯示強抗病種2002-026有一段位於1500 bp附近的DNA分子標誌，而其他感病品系則無。我們期望此研究可作為未來篩選抗病品系或抗病基因的基礎。

Water spinach (Ipomoea aquatica Forsk.), an important summer vegetable in Taiwan, is easily infected by Albugo ipomoeae-aquaticae, in the result of white rust disease. By cotyledon inoculation, our result shows ‘white-seed Jwu Yeh’ lines were most strongly resistant to white rust disease. We also surveyed several factors of water spinach to evaluate the association with disease proportion; Such as stomatal density of cotyledon, contents of photosynthetic pigments (chlorophyll a, chlorophyll b, and carotenoids), concentration of glucose and fructose had positive correlation with disease proportion of juvenile stage in non-linear regression. On the other hand, the concentration of sucrose became negative correlation in simple linear regression. The XY scattered graphs of stomatal density, chlorophyll a, chlorophyll b, glucose and fructose with disease proportion were suggested that they exhibited a grouping relationship. Besides, there was multiple regression correlation between six horticultural characteristics and disease severity. From r2, the predictive power of the multiple regression equation would exceed 0.6. We further used Random Amplified Polymorphic DNA (RAPD) to analyze the different banding patterns between one strong resistance line, 2002-026, and other susceptive lines. The random primer O15 produced an unique band (about 1500 bp) which is present only in the lane of DNA amplified from line, 2002-026, but absent in others. We hope the results of our investigation could help in the further research on resistance breeding and defense gene screening.

台灣植物誌編輯委員會。1978。台灣植物誌第四卷，p. 363。
江明潔。1994。蕹菜鎘結合蛋白基因之篩選與分析。碩士論文。台中：中興大學分子生物研究所。
李明遠。1998。蔬菜病蟲害防治彩色圖冊，三版，p. 116-117。江西科學技術出版社，江西。
李敏郎。2003。莧白銹菌之生物特性及其病害防治研究。博士論文。台中：國立中興大學植物病理研究所。
吳文希。1987。植物病害防治學，一版二印，p. 59。，茂昌，台北。
周廷光。1975。蔬菜主要病害彩色圖鑑，初版，p. 51-52。淑馨，台北。
林試所。1999。台灣真菌名錄，初版，p. 31。農委會，台北。
范淑貞、張粲如、李聯興。1999。蕹菜新品種「桃園一號」之選育。蔬菜作物試驗研究彙報第九輯，p. 483-503。鳳山熱帶園藝試驗分所，高雄。
柯如營。2002。蕹菜白銹菌對蕹菜植株光合作用與醣類代謝之影響。碩士論文。台北：國立台灣師範大學師生物研究所。
許霖慶、徐是雄。1981。香港食用植物，p. 24。市政局出版，香港。
許苑培。1999。探討蕹菜宿根栽培。蔬菜作物試驗研究彙報第九輯，p.504-511。鳳山熱帶園藝試驗分所，高雄。
梁金蘭。1997。蔬菜病蟲實用原色圖譜，p. 172-173。河南科學技術出版社，河南。
黃振文、孫守恭。1998。旋花科蔬菜病害。植物病害彩色圖鑑第二集蔬菜病害 P.112-118。
黃文理、黃祥益、黃炳龍、蔡奇助。2004。利用RAPD分子標誌技術進行台灣芋品系親緣之鑑定。中華農學會報5(4):318-327。
劉政道。1983。專業栽培蔬菜30種，增定四版，p. 154-163。豐年社，台北。
劉政道、李碩朋。1995。台灣農家要覽 農作篇（二），p. 339-344。豐年社，台北。
澤田兼吉(Sawada K.)。1922。台灣總督府中央研究所農業部報告第二號：台灣產菌類調查報告第二編，p. 27-31。
諶靜吾。1985。台灣蔬菜未來發展之展望。夏季蔬菜生產改進研討會專輯，p. 9-14。桃園農改場，桃園。
蘇振良。1999。蕹菜白銹病之探討。國科會：大專生參與專題研究計畫成果報告。台北：國立台灣師範大學生物系。
Agrios, G. N. (1988). Measurment of plant disease. In “Plant Pathology”, 3rd edition, p.164. Academic Press, America.
Chou, H. M., Bundock, N., Rolfe, S. A., and Scholes, J. D. (2000). Infection of Arabidopsis thaliana leaves with Albugo candida (white blister rust) causes a reprogramming of host metabolism. Moleculat Plant Pathology 1(2):99-113.
Edie, H. H., and Ho, B. W. C. (1970). Factors affecting sporangial germination in Albugo ipomoeae-aquaticae. Transations British Mycological Society 55(2):205-216.
Gacutan, M. V. C., and Quimio, T. H. (1978). Studies on the white rust disease of Kangkong (Ipomoea aquatica Forsk.). Philippine Phytopathology 14:69-77.
Gao, R. X., Zhang, C. Q., Chen, M. Y., Chen, X. C., and Zu, K. X. (1985). A preliminary report on the integrated control of water spinach white rust in Fuzhou, Fujian Province, China. Journal of Fujian Agriculture Collage 14:82-86.
Herbers, K., Meuwly, P., Frommer, W. B., Metraux, J-P., and Sonnewald, U. (1996). Systemic acquired resistance mediated by the ectopic expression of invertase: possible hexose sensing in the secretory pathway. The Plant Cell 8:793-803.
Ho, B. W. C., and Edie, H. H. (1969). White rust (Albugo ipomoeae-aquaticae) of Ipomoea aquatica in Hong Kong. Plant Disease Reporter 53:959-962.
Kajornchaiyakul, P., and Brown, J. F. (1976). The infection process and factors affecting infection of sunflower by Albugo tragopogi. Transations British Mycological Society 66(1):91-95.
Kao, W. Y., Tsai, T. T., and Chen, W. H. (1998). Response of photosynthetic gas exchange and chlorophyll a fluorescence of Miscanthus floridulus (Labill) Warb. to temperature and irradiance. Journal of Plant Physiology 152(4-5):407-412.
Kao, W. Y., Tsai, T. T., and Chen, W. H. (1997). The responses of photosynthetic gas exchange and chlorophyll a fluorescence to changes of irradiance and temperature in two species of Miscanthus. Photosynthetica 34(4):497-504.
Khoo, K. L. and Lim, G. (1989). White rust disease of Ipomoea aquatica in Singapore. Journal of Plant Protection in the Tropics 6(2):119-122.
Ko, R.Z., Chen, M.J., and Chou, H.M. (2000). Studies on morphology and physiology of white rust disease of Ipomoea aquatica. Plant Pathology Bulletin 9(4): 177.
Lichtenthaler, H.K., and Wellburn A.R. (1983). Determination of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochemical Society Transactions 11: 591-592.
Lubani, K. R., and Linn, N. B. (1962). Entrance and invasion of the asparagus plant by urediospore germtubes and hyphae of Puccinia asparagi. Phytopathology 52: 115.
Murillo, I., Roca, R., Bortolotti, C., and Segundo, B. S. (2003). Engineering photoassimilate partitioning in tobacco plants improves growth and productivity and provides pathogen resistance. The Plant Journal 36:330-341.
Safeeulla, K. M. and Thirumalachar, M. J. (1953). Morphological and cytological studies in albugo species on Ipomoea aquatica and Merremia emarginata. P.225-231
Scholes, J.D., Lee, P.J., Horton, P., and Lewis, D.H. (1994). Invertase: understanding changes in the photosynthetic and carbohydrate metabolism of barley leaves infected with powdery mildew. New Phytologist 126: 213-222.
Vakalounakis, D.J. and Williams, P. H. (1989). A cotyledon screen for resistance to scab (Cladosporium cucumerinum) in cucumber (Cucumis sativus) seedlings. Ann. Appl. Biol. 115: 443-450.