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研究生: 鄭易濰
Zheng, Yi-Wei
論文名稱: 納豆枯草桿菌共同發酵黑豆及糙薏仁混合物減緩高脂高果糖飲食誘導小鼠之代謝紊亂
Co-fermented black soybean and dehulled adlay with Bacillus subtilis natto mixture ameliorates metabolic disorders in high-fat high-fructose diet-fed mice
指導教授: 蔡帛蓉
Tsai, Po-Jung
潘敏雄
Pan, Min-Hsiung
口試委員: 何元順
Ho, Yuan-Soon
黃步敏
Huang, Bu-Miin
羅翊禎
Lo, Yi-Chen
潘敏雄
Pan, Min-Hsiung
蔡帛蓉
Tsai, Po-Jung
口試日期: 2024/07/17
學位類別: 碩士
Master
系所名稱: 營養科學碩士學位學程
Graduate Program of Nutrition Science
論文出版年: 2024
畢業學年度: 112
語文別: 中文
論文頁數: 138
中文關鍵詞: 代謝症候群胰島素阻抗納豆枯草桿菌γ-聚麩胺酸腸道菌相
英文關鍵詞: Metabolic syndrome, insulin resistance, Bacillus subtilis natto, γ-polyglutamic acid (γ-PGA), gut microbiota
研究方法: 實驗設計法
DOI URL: http://doi.org/10.6345/NTNU202401385
論文種類: 學術論文
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  • 第一章 緒論 1 第二章 文獻回顧 2 第一節 代謝症候群 (Metabolic syndrome, MetS) 2 一 簡介及定義 2 二 成因與危害 3 三 預防與治療 4 第二節 高脂飲食誘發肥胖 5 一 肥胖引起代謝紊亂 5 二 肥胖與血糖調控及胰島素阻抗間之關係 13 第三節 動物實驗模式 15 一 高脂高果糖飲食加劇代謝紊亂 15 二 高脂高果糖飲食對菌相之影響 16 第四節 台灣雜糧農作物-黑豆與糙薏仁 17 一 黑豆 17 二 糙薏仁 18 第五節 發酵食品與納豆菌 19 一 發酵食品定義、目的及功效性 19 二 納豆枯草桿菌簡介 21 第三章 實驗目的與架構 23 第一節 實驗目的 23 第二節 實驗架構 24 第四章 材料與方法 26 第一節 實驗材料 26 一 實驗儀器 26 二 藥品與試劑 27 三 分析套組 27 四 抗體 28 第二節 樣品製備及成份分析 29 一 FBA成份與來源 29 二 FBA製備流程 29 三 食物組成成份與分析方法 31 四 生物活性分析方法 31 第三節 動物實驗 (in vivo) 方法 32 一 動物品系及組別設計 32 二 飼料及果糖溶液配方 33 三 空腹血糖測定 36 四 血清胰島素含量測定 36 五 口服葡萄糖耐受性試驗 37 六 動物犧牲 38 七 組織切片染色 39 八 肝臟三酸甘油酯含量測定 41 九 血清脂肪激素含量測定 42 十 肝臟細胞激素含量測定 43 十一 組織均質及蛋白質萃取 44 十二 蛋白質定量 45 十三 西方墨點法 46 十四 微生物體全長16S擴增子定序分析 48 十五 短鏈脂肪酸含量分析 49 第四節 統計分析 51 第五章 結果與討論 52 第一節 樣品營養成分與生物活性分析 52 一 樣品之營養成分分析 52 二 樣品之總多酚含量 52 三 樣品之 DPPH 自由基清除能力 52 四、FBA 之 γ-PGA 含量 53 第二節 動物實驗 (in vivo) —以HFFD誘發C57BL/6J 小鼠代謝症候群 53 一 FBA 改善小鼠之體重變化 54 二 FBA 對於小鼠攝食量及飲水量之影響及熱量計算表 55 三 FBA 對於小鼠臟器及白色脂肪外觀之影響 56 四 FBA 對於小鼠之血液生化數值表現 59 五 FBA 調節小鼠之空腹血糖 61 六 FBA 增加小鼠之葡萄糖耐受性 62 七 FBA 減緩小鼠之胰島素阻抗 63 八 FBA 調節小鼠肝臟血糖代謝 64 九 FBA 改善小鼠之血清脂肪激素 67 十 FBA 對於小鼠之肝臟組織與三酸甘油酯影響 68 十一 FBA 調節小鼠之肝臟細胞激素 69 十二 FBA 調節小鼠之肝臟脂質代謝 70 十三 FBA 對於小鼠之脂肪細胞大小影響 72 十四 FBA 調節小鼠之脂肪組織脂質代謝 73 十五 FBA 對於小鼠之腸道菌相 α及 β 多樣性影響 75 十六 FBA 對於小鼠之腸道菌相組成影響 76 十七 FBA 提升小鼠之盲腸糞便短鏈脂肪酸 81 第六章 結論 84 第七章 圖表 88 第八章 參考文獻 117

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