本研究藉由微機電製程技術(micro electro mechanical system ,MEMS)製作單管柱氣相微層析晶片與多管柱平行分離氣相微層析晶片(μ-column)，微層析晶片大小為3 cm × 3 cm，選擇Stop-flow GC × GC為系統架構，配合多管柱微層析晶片為第二維分離管柱，且塗佈不同選擇性的靜相材料(DB-1、OV-225、OV-210)於各管柱中，建構Stop-flow GC × GCs系統，第一維微層析晶片無法分離的混合物，可利用第二維不同選擇性的三個分離管柱分離，且同步得三張不同結果的3D層析圖譜，此模組可以最短管柱分離複雜混合物，各層析圖譜流經的管柱長僅4公尺。
選擇14種沸點差距70 ℃揮發性有機氣體(VOCs)為待測氣體，其中有四組混合物無法被第一維(DB-1)微層析晶片分離，且每一組混合物僅含兩種化合物，利用第二維微層析晶片的三個管柱便能有效分離，更進一步挑戰17種沸點差距47 ℃的混合物進行分離測試，此組混合物有兩組訊號峰各含有7種與3種化合物，複雜度較14種化合物高，更能表現此膜組的分離效果。
任一化合物在固定實驗參數下進行實驗，3組3D層析圖譜可得3組座標(x，y)，x與y座標為第一維與第二維微層析晶片滯留時間，3組座標可做為其身分證明並建立資料庫，配合3組3D層析圖譜可有效進行定性分析。

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