梔子為常用中藥材，有清熱、瀉火、涼血等作用。本研究第一部分目的在開發棍子藥材之高球能液相層析方法。梔子藥材中主要成分皆為含醣基化合物，本實驗以磷酸/磷酸鹽的緩衝溶液為沖提系統及5C18分離管柱，於240nm及440nm的偵測波長與線性梯度沖提程式下，在60分鐘內完成梔子藥材中六種含醣基化合物的定量工作。此外，分離管柱的選擇、移動相的鹽類濃度與有機修飾劑的比例等，對梔子藥材中各成分分離效果的影響在文中皆有詳盡的探討。由於市售棍子分為山梔子及水梔子兩不同品種，本研究亦對其中藥效成分含量作一探討評估。
本研究第二部分為中藥製劑黃連解毒湯之分析方法開發，該製劑由梔子、黃連、黃苓、黃柏等四種藥材組成，本分析方法共可定量其中黃連與黃柏藥材的六種四級銨鹽成分，黃苓藥材的四種黃酮類成分，以及棍子藥材的四種含醣基化合物。分析條件之沖提系統由磷酸/磷酸鹽緩衝溶液及氯甲烷/水所組成，分離管柱為5C18-MS，偵測波長定於 240、280及440nm，利用適當的梯度沖提程式可在60分鐘內，順利定量此製劑中十四種指標成分。影響分離效果的因素，包括沖提液中的有機相組成、磷酸鹽濃度及pH值等在文中均有詳細探討。

Gardenia jasminoid Ellis is a commonly used Chinese herbal drug which is used in traditional Chinese medicine as an antiphlogistic, diuretic, laxative, choleretic, and hemostatic in the treatment of traumatosis by external application. In this research, a high-performance liquid chromatographic method for the determination of six components was developed within 60 min. This method was carried out by using a gradient solvent system of phosphate buffer-acetonitrile-methanol and detecting at 240 nm and 440 nm. The effects of column selectivity, phosphate concentration and organic modifier concentration of the mobile phase on the retention and separation of the solutes were also studied.
Huang-lien-chieh-tu-tang, a Chinese herbal preparation, is known to consist of four herb drugs. In this part, a total of 14 components which included 6 quartemary alkaloids in Coptidis rhizoma and Phellodendri cortex and 4 flavones in Scutellariae radix and 4 glucosides in Gardeniae fructus could be determinated by a LC method using gradient solvent system that contained phosphate buffer. The effects of mobile phase and column selectivity were also discussed.