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Author: 黃靖雅
Huang, Ching-Ya
Thesis Title: Lunasin對C2C12肌小管細胞其發炎反應與葡萄糖利用之探討
The effects of lunasin on inflammatory response and glucose utilization in C2C12 myotubes
Advisor: 謝佳倩
Hsieh, Chia-Chien
Committee: 許珊菁 吳啟豪 謝佳倩
Approval Date: 2021/07/14
Degree: 碩士
Master
Department: 營養科學碩士學位學程
Graduate Program of Nutrition Science
Thesis Publication Year: 2021
Academic Year: 109
Language: 中文
Number of pages: 88
Keywords (in Chinese): LunasinC2C12肥胖抗發炎胰島素阻抗
Keywords (in English): Lunasin, C2C12, obesity, Anti-inflammatory, insulin resistance
Research Methods: 實驗設計法
DOI URL: http://doi.org/10.6345/NTNU202101379
Thesis Type: Academic thesis/ dissertation
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  • 第一章 研究動機與目的 1 第二章 文獻探討 2 第一節、肥胖 2 一、定義 2 二、流行病學調查 2 三、肥胖與衍生疾病 3 四、肥胖與代謝症候群 4 五、肥胖與發炎 5 六、肥胖與胰島素阻抗 10 第二節、Lunasin 15 一、簡介 15 二、Lunasin功能介紹 16 第三節、研究方法 20 一、C2C12小鼠肌肉纖維母細胞 20 二、3T3-L1小鼠纖維母細胞 21 三、棕櫚酸誘發胰島素阻抗模式 22 第四節、研究架構 23 第三章 材料與方法 24 第一節、實驗設備及耗材 24 一、實驗儀器設備 24 二、拋棄式無菌耗材 24 第二節、實驗材料與方法 25 一、Lunasin處理對C2C12小鼠肌肉纖維母細胞之細胞存活率影響 25 二、肥胖模式下,Lunasin處理對C2C12小鼠肌小管細胞發炎介質的影響 30 三、肥胖模式下,Lunasin處理對C2C12小鼠肌小管細胞對葡萄糖攝入以及胰島素敏感性的影響 36 四、統計分析 39 第四章 實驗結果 40 第一節、Lunasin處理對C2C12肌肉纖維母細胞之細胞存活率影響 40 一、Lunasin處理對C2C12肌肉纖維母細胞和肌小管細胞存活率影響 40 二、Lunasin處理對C2C12肌小管細胞的攝入情形 42 三、PA處理對C2C12肌小管細胞之細胞存活率影響 44 四、Ad-CM處理對C2C12肌小管細胞之細胞存活率影響 45 第二節、肥胖模式下,lunasin處理對C2C12肌小管細胞發炎介質分泌影響 46 一、Lunasin處理對C2C12肌小管細胞對發炎性基因之影響 46 二、Lunasin處理對C2C12肌小管細胞對發炎性細胞激素之影響 48 三、Lunasin處理對C2C12肌小管細胞對細胞內氧化壓力之影響 51 四、Lunasin處理對C2C12肌小管細胞對細胞活性之影響 54 第三節、肥胖模式下,Lunasin處理對C2C12肌小管細胞葡萄糖攝取影響 58 一、Lunasin處理C2C12肌小管細胞對2-NBDG葡萄糖攝取之影響 58 二、Lunasin處理對C2C12肌小管細胞胰島素敏感性之影響 61 三、Lunasin處理對C2C12肌小管細胞肝醣貯存之影響 64 四、Lunasin處理對C2C12肌小管細胞GLUT4表現之影響 65 第五章 討論 68 一、Lunasin處理對C2C12肌小管細胞對發炎性細胞激素之影響 68 二、Lunasin處理對C2C12肌小管細胞細胞內氧化壓力與細胞活性之影響 69 三、Lunasin處理對C2C12肌小管細胞葡萄糖代謝及胰島素敏感性之影響 70 四、Lunasin處理對C2C12肌小管細胞肝醣之影響 72 第六章 結論 74 附錄 75 參考文獻 77

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