有關乳突瘤病毒 (papillomaviruses ； PVs) 複製的資訊，一般均由
研究 bovine papillomavirus type 1 (BPV 1) 而來； 對於 Human
papillomaviruses (HPVs) DNA 複製的了解則相當有限。 研究 HPVs 複製
所遭遇的一大困難是無法找到一個可以使 HPVs 穩定複製的細胞培養系統
，因而無法對其複製的情形做獨立的觀察。 1980 年，榮總胡承波博士建
立了一子宮頸癌細胞株 CC7T ，其內同時含有 HPV16 插入式以及穩定存在
的表體 DNA ，為研究 HPVs 複製提供了一個良好的材料。此外，表體 DNA
與 prototype 在核甘酸序列上，亦有些許差異，發生變異的地方，正好是
與 HPV 複製有關的基因所在之處 ----E1 、 E7 。為判別此變異是否為表
體 DNA 能穩定存在於 CC7T 中的主因，本實驗用 CC7T 中選殖所得的表體
DNA (pCC7T-BS) 以及 prototype HPV 16 DNA (pHPV-BS) 分別 transfect
至其他子宮頸癌 (HeLa) 或咽喉癌細胞株 (Hep-2) 內，觀察並比較二者在
其他細胞株內複製的情形如何。 實驗結果發現， transfection 之後五天
內，除了以 pCC7T-BS transfection 之細胞株 HeLa 發生生長遲滯及細胞
死亡之外， 其他實驗組並沒有明顯的細胞死亡現象，同時，細胞的型態也
沒有發生改變。 在抽取小分子量的 DNA做分析時發現：無論是 pCC 7T-BS
或是 pHPV-BS ，在 HeLa 及 Hep-2 內複製的情形並沒有很大的差異， 均
是隨著天數的增加， DNA 的量漸趨減少；同時，二者在細胞株中亦沒有複
製的現象。 由此推論，表體核甘酸序列上的變異，對於其穩定的複製與存

Observing the replication of human papillomavirus is difficult
because of lacking an in vitro system to support a stable
episomal viral DNA replication. In 1980, Dr.C. P. Hu established
a cervical carcinoma cell line CC7T, which contains both episomal
and integrated HPV 16 DNA. Compare the DNA sequences with the
prototype HPV 16 DNA, there are point mutations in both E1 andE7
ORFs in epi some, and these two genes are responsible for viral
DNA replication. In order to prove whether these mutations could
effect the existence of episomal DNA in CC7T, we transfected the
cloned episome (pCC7T-BS) and the prototype HPV 16 DNA (pHPV-BS)
into re lated cervical carcinoma and larynx carcinoma cell lines,
HeLa and Hep-2, respectively. Our results showed that there were
neither apparent changes in morphology nor cell death in
transfected cells except pCC7T-BS transfected cell line HeLa. In
HeLa ， t wo days after transfection with pCC7T-BS ， the growth
of the cells delayed and the cells appeared to die 。 On the
other hand ， the replication patterns of both pCC7T-BS and
pHPV-BS were similiar in HeLa and Hep-2. The amount of DNA were
papillomavirus replication reduced gradually a Most of the
informations about nd there were no apparent DNA replication in
both cell lines. It indicated that the mutations occured in
episome might not as important as we expected. come from
researches of the bovine papillomavirus type 1.
Observing the replication of human papillomavirus is difficult