核苷酸化合物是生物系統中作為能量來源的基本生物分子，由核糖、含氮鹼基及磷酸基團組成，存在於生物體如植物、動物、細菌當中。金屬有機骨架（MOFs）由於其高表面積和均勻的孔徑，在吸附領域當中有廣泛的應用。近年来的文獻顯示出MIL-101-NH2 (Al) 從水中去除磷酸鹽的顯著潛力，而腺苷核苷酸化合物結構上帶有磷酸基團。本研究中，合成MIL-101-NH2 (Al)及MIL-160 (Al)並對其進行表徵。同時選擇 MIL-101-NH2 (Al)作為分散式固相萃取(Dispersive Solid-Phase Extraction, dSPE)的吸附劑，以 MIL-160 (Al)作為對照。使用cyanopropyl(CN)管柱並搭配超高效液相層析串聯質譜(UPLC-MS/MS)系統分對AMP、ADP 和 ATP三個核苷酸化合物進行分析。MOF搭配分散式固相萃取法進行優化結果表明，結果顯示在酸性環境下AMP、ADP 和 ATP 能夠被成功脫附出來。同時層析上能在14分鐘內實現了良好的分離，並且具有良好的準確性(82-121%)及精密度(0.4%-14.3%)，線性範圍為 1-1000 ng/mL，相關係數r > 0.995。偵測極限和定量極限分別為 0.5 ng/mL 和 1 ng/mL。本研究開發的基於 MOF 的 dSPE 搭配UPLC-MS/MS 方法在食品或生物樣品中具有巨大的應用潛力。

Nucleotides are essential biomolecules that serve as sources of energy in biological systems. Metal-organic frameworks (MOFs), have garnered significant attention due to their high surface area and uniform pore size. The MOFs is an ideal material for adsorp-tion applications, which have demonstrated significant potential of MIL-101-NH2 (Al) for phosphate removal from water. In this study, MIL-101-NH2 (Al) and MIL-160 (AI) were synthesized and characterized. MIL-101-NH2 (Al) selected as dispersive solid-phase extraction (dSPE) adsorbent, MIL-160 (AI) was used for comparison. The MOF-based dSPE method exhibited excellent efficiency in eluting nucleotides under acidic conditions. Ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) system was used for the determination of AMP, ADP and ATP. A good separation was achieved within 14 minutes, demonstrating high accuracy (82%-121%) and precision (0.4%-14.3%), with a linear range of 1-1000 ng/mL and correla-tion coefficients (r) > 0.995. The limit of detection (LOD) and limit of quantification (LOQ) were determined to be 0.5 ng/mL and 1 ng/mL, respectively. The MOF-based dSPE and UPLC-MS/MS method developed in this study holds immense potential for further applications in food or biological samples.

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