研究生: |
詹哲泓 |
---|---|
論文名稱: |
光學同調斷層造影術對輻射誘發腫瘤細胞侵襲能力之定量分析 |
指導教授: | 郭文娟 |
學位類別: |
碩士 Master |
系所名稱: |
光電工程研究所 Graduate Institute of Electro-Optical Engineering |
論文出版年: | 2008 |
畢業學年度: | 96 |
語文別: | 英文 |
論文頁數: | 41 |
中文關鍵詞: | 子宮頸癌 、肝癌 、侵襲能力 、放射線 |
英文關鍵詞: | Cervical cancer, Hepatocellular carcinoma, Invasion, Radiation |
論文種類: | 學術論文 |
相關次數: | 點閱:123 下載:3 |
分享至: |
查詢本校圖書館目錄 查詢臺灣博碩士論文知識加值系統 勘誤回報 |
實驗中有效的利用三維快速高解析度掃描式光學同調斷層儀(SS-OCT)系統,來分析肝癌細胞(HCC)經過放射線照射後之侵犯能力。 量測過程中,分別在0、12、24、48 (hrs) 等不連續的時間點,利用SS-OCT系統來取得多張2維的光學同調斷層(OCT)影像。 實驗結果我們發現大部分細胞集中在膠的表層(depth<0.18 mm),僅有少部份具有較高侵犯能力的細胞能遷移至較深層(depth>0.18 mm)的部位。 故本研究利用SS-OCT之非侵入式的優點,分別針對受放射線刺激前後的細胞,對其侵犯程度的特性及遷移距離進行量化分析。
[1] Fu K K, Phillips T L 1991 Biologic rationale of combined radiotherapy and chemotherapy Hematol Oncol Clin North Am 5 737–51
[2] Cheng J C, Chuang V P, Cheng S H, Lin Y M, Cheng T I, Yang P S et al. 2001 Unresectable hepatocellular carcinoma treated with radiotherapy and/or chemoembolization Int J Cancer 96 243-52
[3] Liang S X, Zhu X D, Lu H J, Pan C Y, Li F X, Huang Q F et al. 2005 Hypofractionated three-dimensional conformal radiation therapy for primary liver carcinoma Cancer 103 2181-8
[4] Wild-Bode C, Weller M, Rimner A, Dichgans J, Wick W 2001 Sublethal irradiation promotes migration and invasiveness of glioma cells: implications for radiotherapy of human glioblastoma Cancer Res 61 2744–50
[5] Camphausen K, Moses M A, Beecken W D, Khan M K, Folkman J, O’Reilly M S 2001 Radiation therapy to a primary tumor accelerates metastatic growth in mice Cancer Res 61 2207–11
[6] Wei L H, Lai K P, Chen C A, Cheng C H, Huang Y J, Chou C H, Kuo M L, and Hsieh C Y 2005 Arsenic trioxide prevents radiation-enhanced tumor invasiveness and inhibits matrix metalloproteinase-9 through downregulation of nuclear factor kB Oncogene 24 390-8
[7] Cheng JC H, Chou C H, Kuo M L, and Hsieh C Y 2006 Radiation-enhanced hepatocellular carcinoma cell invasion with MMP-9 expression through PI3K/Akt/NF-kB signal transduction pathway Oncogene 25 7009-18
[8] Huang D, Swanson E A, Lin C P, Schuman J S, Stinson W G, Chang W, Hee M R, Flotte T, Gregory K, Pufialito C A, and Fujimoto J G 1991 Optical coherence tomography Science 254 1178-81
[9] Boppart S A, Brezinski M E, Bouma B E, Tearney G J, and Fujimoto J G 1996 Investigation of developing embryonic morphology using optical coherence tomography Developmental Biology 177 54–63
[10] Boppart S A, Bouma B E, Pitris C, Southern J F, Brezinski M E, and Fujimoto J G 1998 In vivo cellular optical coherence tomography imaging Nature Medicine 4, 861–4
[11] Choma M A, Sarunic M V, Yang C H, and Izatt J A 2003 Sensitivity advantage of swept source and Fourier domain optical coherence tomography Optics Express 11 2183-9
[12] Yun S H, Boudoux C, Tearney G J, and Bouma B E 2003 High-speed wavelength-swept semiconductor laser with a polygon-scanner-based wavelength filter Optics Letters 28 1981-3
[13] Yun S H, Tearney G J, de Boer J F, Iftimia N, and Bouma B E 2003 High-speed optical frequency-domain imaging Optics Express 11 2953-63
[14] Breuls R G M, Mol A, Petterson R Oomens C W J, Baaijens F P T, and Bouten C V C 2003 Monitoring local cell viability in engineered tissues: A fast, quantitative, and nondestructive approach Tissue Eng. 9 269-81
[15] So P T, Dong C Y, Masters B R, and Berland K M 2000 Two-photon excitation fluorescence micrsocopy Ann. Rev. Biomed. Eng. 2 399-429
[16] Tan W, Oldenburg A L, Norman J J, Desai T A, and Boppart S A 2003 Optical coherence tomography of cell dynamics in three-dimensional tissue models Optics Express 14 7159-71
[17] Leitgeb R, Hitzenberger C K, and Fercher A F 2003 Performance of fourier domain vs. time domain optical coherence tomography Optics Express 11 2183-9
[18] King, Roger John Benjamin 2000 Cancer Biology: Prentice Hall, Second Edition
[19] Cancer facts and figures 2008 American Cancer Society
http://www.cancer.org/docroot/home/index.asp