目的：探討長期增補複方炙甘草湯後進行單次高強度運動，對氧化壓力的變化與心血管疾病的影響。方法：以18位健康大學男性，依VO2max高低為次序平衡之依據分為兩組：炙甘草組與安慰劑組，實驗處理為期四週。以高強度運動 ( 85％VO2max ) 評估增補後對氧化壓力的變化。在增補前、後與運動前、後分別採集血液樣本分析catalase ( CAT ) 活性、superoxide dismutase ( SOD ) 活性、malondialdehyde ( MDA ) 濃度、low-density lipoprotein cholesterol ( LDL-C ) 濃度、high-density lipoprotein cholesterol ( HDL-C ) 濃度與oxidize low-density lipoprotein ( oxLDL ) 濃度等生化分析。所得數據以獨立樣本 t 考驗、混合設計雙因子(組別 × 時序)變異數分析 ( ANOVA ) 與混合設計雙因子(組別 × 時序)共變數分析 ( ANCOVA ) 檢定。結果：四週增補後發現紅血球溶胞液CAT與SOD活性、血漿LDL-C與HDL-C在組間與時序的比較皆未達顯著差異。兩組受試者在運動後的血漿乳酸濃度( 7.65 ± 0.90 mM ) 顯著高於運動前 ( 1.51 ± 0.48 mM, p＜.05 )。炙甘草組的血漿MDA ( 2.58 ± 0.39 mM ) 在增補四週後顯著低於安慰劑組 ( 3.61 ± 0.26 mM, p＜.05 )。同樣地，炙甘草組在運動後之MDA ( 1.63 ± 0.11 mM ) 也顯著低於安慰劑組 ( 2.81 ± 0.33 mM, p＜.05 )。炙甘草組在增補後之血漿oxLDL ( 5.62 ± 0.74 U ) 顯著低於增補前 ( 6.56 ± 0.69 U, p＜.05 )。結論：持續四週增補複方炙甘草湯後，可防止從事單次高強度運動後自由基對脂質造成的損傷，有助於保護脂質，降低血漿MDA與oxLDL的濃度，但無法改變血漿HDL-C與LDL-C濃度的變化，推測可能與增補的劑量、時間長短或吸收代謝有關。本實驗推測增補複方炙甘草湯可能有助於預防心血管疾病。

Purpose: To investigate the effects of Fufang Zhigancao Tang supplementation on oxidative damage and cardiovascular risk factors after one bout of high intensive exercise. Methods: Eighteen healthy college males were assigned into two groups ( Zhigancao group and placebo group ) according to VO2max. Experimental treatment period lasted for four weeks. At the end of treatment, a bout of high intensive exercise was performed to observe changes of oxidative stress. Blood samples were collected before, after supplementation and immediately after exercise. Venous blood samples were analyzed for catalase (CAT) activity, superoxide dismutase (SOD) acivity, malondialdehyde (MDA), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C) and oxidized low-density lipoprotein (oxLDL). Independent t test, mixed design of two-way ( group × time ) ANOVA and ANCOVA were used for data analysis. Results: After four weeks of supplementation, CAT activity, SOD activity, LDL-C and HDL-C in erythrocyte lysate showed no significant differences between groups and times. Plasma lactate was significantly higher after exercise ( 7.65 ± 0.90 mM ) than that of before ( 1.51 ± 0.48 mM, p＜.05 ). Compared with placebo group ( 3.61 ± 0.2 mM ), plasma MDA in Zhigancao group ( 2.58 ± 0.39 mM ) was significantly lower after supplementation ( p＜.05 ). There was a smiliar result after exercise. Plasma MDA in Zhigancao group ( 1.63 ± 0.11 mM ) after exercise was significantly lower than that in placebo group ( 2.81 ± 0.33, p＜.05 ). Plasma oxLDL in Zhigancao group ( 5.62 ± 0.74 U ) was significantly lower than that in placebo group ( 6.56 ± 0.69 U, p＜.05 ). Conclusion: After four-week supplementation of Fufang Zhigancao Tang, it is effective in preventing lipid damage such as decrease of plasma MDA and oxLDL after a bout of high intensity exercise. However, plasma HDL-C and LDL-C were not changed. It may be related with dosage, treatment duration and metabolism. This study may provide evidence to support potentiality of Fufang Zhigancao Tang on prevention of cardiovascular disease.

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