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研究生: 簡琬宣
Wan-Hsuan Chien
論文名稱: 以形態與分子證據探討紋黃蝶在台灣之分佈
Study on distribution of Colias erate in Taiwan based on morphological and molecular evidence
指導教授: 徐堉峰
Hsu, Yu-Feng
學位類別: 碩士
Master
系所名稱: 生命科學系
Department of Life Science
論文出版年: 2010
畢業學年度: 99
語文別: 中文
論文頁數: 71
中文關鍵詞: 紋黃蝶族群遺傳形質分析分子標記
英文關鍵詞: Colias erate formosana, population genetic, morphometric analysis, molecular marker
論文種類: 學術論文
相關次數: 點閱:160下載:4
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  • 紋黃蝶(Colias erate formosana)在台灣全年分布於中高海拔地區,過去認為高山族群屬於台灣特有亞種;而北部的低海拔地區出現時間則以秋、冬為主,有文獻認為也許源自日本。然而紋黃蝶在北部確實出現的季節、個體來源目前無相關研究。本研究針對北部低海拔的族群進行生態、成蝶形態、族群遺傳等,各方面相關研究,探討台灣產紋黃蝶之分布。
      於幼生期方面,在15~28℃發育的時間隨溫度遞減而花費時間增加。此外,C. erate的食性測試,除了已知的寄主菽草(Trifolium pretense)外,還發現紫苜蓿(Medicago sativa)、印度草木樨(Melilotus indicus)可被幼蟲利用;另外,餵食田菁(Sesbania cannabiana)的幼蟲,則發生存活率降低與羽化成功率低的現象。檢驗日本和台灣低海拔、中海拔共三個地區的樣品,利用形質單變數分析與主成分分析,各地區樣品無法區分,亦無法將北部低海拔族群歸群於任一類群。野外族群調查於2008~2010年進行,結果顯示低海拔樣區在夏季及秋季有紋黃蝶族群記錄;武陵農場則是每次調查都有記錄。本研究利用分子技術(序列分析、SeAFLP、微衛星)探討低海拔個體來源。從COI、EF-1α序列資料,顯示族群間遺傳多樣性低,定序資料無法提供解決;而透過SeAFLP和微衛星實驗,得知台灣低海拔和中海拔族群間缺乏分化(Fst < 0.05),反應基因交流頻繁,可推測兩族群間的阻礙小、移動頻繁。

    The sulfur butterfly Colias erate formosana is mainly distributed at higher elevations in Taiwan, and has been considered as an endemic subspecies. Nevertheless, this Pierid butterfly about was be found at lowlands in northern part of the island in fall and winter. So far there has been insufficient information to explain the source of the individuals found on lowland Taiwan. I applied morphological, ecological and genetic methods to investigate this question.
    With regard to the development of the immature stages, C. erate grows along with the progressively decreasing temperature between 15~28 ℃. Four potential host-plants of C. erate were tested. The results revealed Trifolium pretense, Medicago sativa, and Melilotus indicus can be used successfully by larva. By contrast, the survival rate of larva was reduced considerally by Sesbania cannabiana. The analysis of morphological characters using ANOVA and principal-components failed to distinguish three populations, including the Japan, low elevation and high elevation in Taiwan. A censor was performed at during 2008 and 2010, showing that the present of C. erate was unstable at Bisha Fishing Port but persisting at Wuling Farm was stable all year. The molecular technology used included sequential analysis, SeAFLP and microsatellite. It turned out that generic diversity were low for COI and EF-1α, that this methodology is not suitable for this research. SeAFLP and microsatellite demonstrated no differention was found between was found between low elevation and high elevation, and the results confirmed that the movement between two elevations occurred frequently.

    目次………………………………………………………………………1 附表次……………………………………………………………………2 附圖次……………………………………………………………………3 附錄………………………………………………………………………4 中文摘要…………………………………………………………………5 英文摘要…………………………………………………………………6 前言…………………………………………………………………7 材料與方法…………………………………………………………12 結果…………………………………………………………………21 討論…………………………………………………………………29 參考文獻……………………………………………………………35 附表…………………………………………………………………42 附圖……………………………………………………………………54 附錄……………………………………………………………………66

    山中正夫。1972。台灣產蝶類の分布(2)。日本鱗翅學會會報,蝶與蛾 23, Suppl.1:29-30。
    白水隆。1960。原色台灣蝶類大圖鑑。保育社,68-69頁。
    邱秀婷。2003。黃蝶寄主植物偏好分析。國立台灣師範大學生物系碩士論文。
    林唯穎。2003。青斑蝶族群遺傳結構之研究。國立台灣大學昆蟲學系碩士論文。
    武春生。2010。中國动物志昆虫纲第五十二卷鳞翅目粉蝶科。科學出
    版社,59-64頁。
    徐堉峰。2006。台灣蝶類圖鑑第三卷。台灣省立鳳凰谷鳥園,150-156頁。
    徐德華。2009。台灣鮭魚的分子遺傳標記。國立台灣海洋大學水產養殖學系碩士論文。
    梁玉琦。2004。台灣生態區分區之研究。國立台灣大學森林環境暨資源學系碩士論文。
    陳素瓊、歐陽盛芝、王筱瑗、黃校翊、何昇儒、黃信傑、施佳佑、陳
    文杰。2003。溫度對大白斑蝶(Idea leuconoe clara(Butler))
    (鱗翅目:斑蝶科)發育之影響。台灣昆蟲 23:331-351。
    陳建志。2009。玉山國家公園移動性斑蝶族群結構與週邊地區相關性探討。玉山國家公園管理處。
    鹿野忠雄。1928。台灣產蝶類分布資料其の六。台灣博物學會會報 18(96):203-205。
    濱野榮次。1987。台灣蝶類生態大圖鑑。牛頓出版社,474頁。
    闕宏軒。2002。從形態與生理證據重新探究密紋波眼蝶複合群的分類問題。國立台灣師範大學生物系碩士論文。
    關崇智。1991。昆蟲生理學。國立編譯館。台北,528-530頁。
    羅尹廷。2001。夸父綠小灰蝶之生態學初探。國立台灣師範大學生物系碩士論文。
    Abbas, M., Rafi, M. A., Inayatullah, M., Khan, M. R. & Pavulaan, H. 2002. Taxonomy and distribution of butterflies (Papilionoidea) of the Skardu region, Pakistan. The Taxonomic Report of the International Lepidoptera Survey 3, Num.9.
    An, J., Bechet, A., Berggren, Å., Brown, S. K., Bruford, M. W., Cai, Q., Cassel-Lundhagen, A., Cezilly, F., Chen, S. L., Cheng, W., Choi, S. K., Ding, X. Y., Fan, Y., Feldheim, K. A., Feng, Z. Y., Friesen, V. L., Gaillard, M., et al. 2010. Permanent genetic resources added to Molecular Ecology Resources database 1 October 2009–30 November 2009. Molecular Ecology Resources 10(2): 404–408.
    Avise, J. C., Arnold, J., Ball, R. M., Bermingham, E., Lamb, T., Neigel, J. E., Reeb, C.A. & Saunders, N. C. 1987. Intraspecific phylogeography: the mitochondrial DNA bridge between population genetics and systematic. Annual Review of Ecology and Systematics 18: 489-522.
    Bensch, S. & Åkesson, M. 2005. Ten years of AFLP in ecology and evolution: why so few animals? Molecular Ecology 14(10): 2899-2914.
    Berres, M. E. 2002. General fluorescent AFLP(fAFLP) protocols. URL
    http://ravel.zoology.wisc.edu/sgaap/AFLP_html/fAFLP_protocols.htm
    Breuker, C. J., Gibbs, M., Van Dongen, S., Merckx, T., & Van Dyck, H.  
      2010. The use of geometric morphometrics in studying butterfly
      wings in an evolutionary ecological context. Morphometrics for Nonmorphometricians 124: 271-287.
    Brower, A. V. Z. & Jeansonne, M. M. 2004. Geographical populations and “subspecies” of new world butterflies (Nymphalidae) share a recent origin and are not phylogenetically distinct. Annals of the Entomological 97(3): 519-523.
    Caterino, M. S. & Sperling, F. A. H. 1999. Papilio phylogeny based on
    mitochondrial cytochrome oxidase I and II genes. Molecular
    Phylogenetics and Evolution 11(1): 122–137.
    Clement, M., Posada, D. & Crandall K. A. 2000. TCS: a computer program toestimate gene genealogies. Molecular Ecology 9(10): 1657-1659.
    Dingle, H.& Drake, V. A. 2007. What is migration? BioScience 57(2):
    113-121.
    Excoffier, L. & Lischer, H. L. 2010. Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under linux and windows. Molecular Ecology Resources 10(3): 564-567.
    Grieshuber, J. & Lamas, G. 2007. A synonymic list of the genus Colias Fabricius,1807. Mitteilungen der Münchner Entomologischen Gesellschaft 97: 131-171.
    Habel, J. C., Rödder, D., Schmitt, T. & Nève, G. 2011. Global warming will affect the genetic diversity and uniqueness of Lyceana helle populations. Global Change Biology 17(1): 194-205.
    Hayes, J. L. 1980. The population ecology of a natural population of the
    pierid butterfly Colias alexandra. Oecologia 49(2): 188-200.
    Honda, K., Nishii, W. & Hayashi, N. 1997. Oviposition stimulants for sulfur butterfly Colias erate poliographys: cyanoglucosides as synergistis involved in host preference. Journal of Chemical Ecology 23(2): 323-332.
    Howard, E. & Davis, A. K. 2009. The fall migration flyways of monarch butterflies in eastern North America revealed by citizen scientists. Journal of Insect Conservation 13(3): 279-286.
    Hurst, G. D. D. & Jiggins, F. M. 2005. Problems with mitochondrial DNA as a marker in population, phylogeographic and phylogenetic studies: the effects of inherited symbionts. Proceedings of the Royal Society B: Biological Sciences 272(1572): 1525-1534.
    Iwase, T., 1964. Recent foodplant records of the loochooan butterflies. Journal of the Lepidoptera’s Society 18(2): 105-109.
    Kim, M. I., Wan, X., Kim, M. J., Jeong, H. C., Ahn, N. H., Kim, K. G.,
    Han, Y. S. & Kim I. 2010. Phylogenetic relationships of true
    butterflies (Lepidoptera: Papilionoidea) inferred from COI, 16S
    rRNA and EF-1α sequences. Molecules and Cells 30(5): 409-425.
    Kingsolver, J. G. 1983. Ecological significance of flight activity in Colias butterflies: implications for reproductive strategy and population structure. Ecology 64(3):546-551.o
    Librado, P. & Rozas, J. 2009. DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25(11): 1451-1452.
    Lohmann, K. J., Putman, N. F. & Lohmann, C. M. F. 2008. Geomagnetic imprinting: A unifying hypothesis of long-distance natal homing in salmon and sea turtles. Pnas 105(49): 19096-19101.
    Lu, C. C., Wu, L. W., Jiang, G. F., Deng, H. L., Wang, L. H., Yang, P. S.
    & Hsu, Y. F. 2009. Systematic status of Agehana elwesi f. cavaleriei
    based on morphological and molecular Evidence. Zoological Studies
    48(2): 270-279.
    Malcolm, S. B. 1987. Monarch butterfly migration in North America:
    controversy and conservation. Tree 2(5): 135-138.
    Meudt, M. H. & Clarke, C. A. 2007. Almost forgotten or latest practice? AFLP application, analyses and advances. Trends in Plant Science 12(3): 106-117.
    Monteiro, A. & Pierce, N. E. 2001. Phylogeny of Bicyclus (Lepidoptera:
    Nymphalidae) inferred from COI, COII, and EF-1a gene
    sequences. Molecular Phylogenetics and Evolution 18(2): 264–281.
    Nakanishi, Y., Watanabe, M. & Ito, T. 1996. Differences in lifetime reproductive output and mating frequency of two female morphs of the sulfur butterfly, Colias erate (Lepidoptera: Pieridae). Journal of Research on the Lepidoptera 35: 1-8.
    Narita, S., Nomura, M., Kato, Y., Fukatsu, T. 2006. Genetic structure of sibling butterfly species affected by Wolbachia infection sweep: evolutionary and biogeographical implications. Molecular Ecology 15(4): 1095–1108.
    Narita, S., Shimajiri, Y. & Nomura, M. 2009. Strong cytoplasmic incompatibility and high vertical transmission rate can explain the high frequencies of Wolbachia infection in Japanese populations of Colias erate poliographus (Lepidoptera: Pieridae). Bulletin of Entomological Research 99: 385-391.
    Nei, M. & Tajima, F. 1983. Maximum likelihood estimation of the number of nucleotide substitutions from restriction sites data. Genetics 105(1): 207-217.
    Peakall, R., & Smouse, P. E. 2006. GENALEX 6: genetic analysis in     
      Excel. Population genetic software for teaching and research.   
      Molecular Ecology Notes 6(1): 288-295.
    Plumb, G. E., White, P. J., Coughenour, M. B. & Wallen, R. L. 2009. Carrying capacity, migration, and dispersal in Yellowstone bison. Biological Conservation 142(11): 2377-2387.
    Schneider, F. 1962. Dispersal and migration. Annual Review of Entomology 7: 223-242.
    Shall, J., Lehman, A. & Creighton, L. 2001. JMP Start Statistics. Pacific   
      Grove CA: Duxbury Press.
    Sherman, P. W. & Watt, W. B. 1973. The thermal ecology of some Colias butterfly larvae. Journal of Comparative Physiology 83(1): 25-40.
    Shirôzu, T., 1955. New or little known butterflies from the north-eastern Asia, with some synonymic notes. III. Sieboldia 1(3): 229-236.
    Stefanescu, C., Alarcón, M. & Àvila, A. 2007. Migration of the painted
    lady butterfly Vanessa cardui ,to north-eastern Spain is aided by
    African wind currents. Journal of Animal Ecology 76(5): 888-898.
    Tamura, K., Dudley, J., Nei, M. & Kumar, S. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0. Molecular Biology and Evolution 24(8): 1596-1599.
    Verhulst, J. T. 2000a. Les Colias du Globe, Monograph of the genus Colias Planches-Plates. Goecke & Evers 308pp.
    Verhulst, J. T. 2000b. Les Colias du Globe, Monograph of the genus Colias Texte. Goecke & Evers p.51-56.
    Vos, P., Hoger, R., Bleeker, M., Reijans, M., Van de lee T., Hornes, M., Frijters, A., Pot, J., Peleman, J., Kuiper, M. & Zabeau, M. 1995. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research 23(21): 4407-4414.
    Watanabe, L. & Nakanishi, Y. 1996. Population structure and dispersal of the sulfurbutterfly Colias erate (Lepidoptera: Pieridae) in an isolated plain located in a cool temperate zone of Japan. Japanese Journal of Entomology 64(1): 17-29.
    Webster, M. S., Marra, P. P., Haig, S. M., Bensch, S. & Holmes, R. T. 2002. Links between worlds: unraveling migratory connectivity. Trends in Ecology & Evolution 17(2): 76–83.
    Williams, C. B. 1957. Insect migration. Annual Review of Entomology 2:
    163-180.
    Williams, C. B. 1970. The migrations of the painted lady butterfly, Vanessa cardui (Nymphalidae), with special reference to North America. Journal of the Lepidopterists’ Society 24(3): 157-175.
    Wright, S. 1965. The interpretation of population structure by F-statistics with special regard to systems of mating. Evolution 19(3): 395-430.

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