本研究之目的在開發中藥材茵陳蒿與製劑茵陳蒿湯的高效液相層析法。茵陳蒿藥材中主要藥效成分為flavone、coumarin與酚類衍生物，本實驗以磷酸/磷酸鹽緩衝溶液及水/甲醇/氰甲烷為沖提系統，分離管柱為5C18-MS，於280nm的偵測波長及線性梯度沖提程式下，在85分鐘內完成茵陳萬藥材中十種藥效成分的定量工作，並且藉由市售品的定量分析評估藥材外觀與品質的關係以及比較茵陳萬與北茵陳的差異。此外，分離管柱、移動相與前置管柱之選擇對茵陳萬藥材各成分層析效果的影響在本文中均有詳盡的探討。
中藥製劑茵陳蒿湯是由茵陳蒿、梔子、大黃藥材所組成，本分析方法共可定量茵陳蒿約五個藥效成分、梔子的三個藥效成分及大黃約五個藥效成分。本實驗以磷豉鹽及水/甲醇/氰甲烷為沖提系統，分離管柱為5C18-MS，於250nm的偵測波長及線性梯度沖提程式下，在80分鐘內完成中藥製劑茵陳蒿湯中十三種成分的定量工作，並且定量十批市售品以及比較藥效成分在不同前處理方式下的差異。此外，分離管柱、移動相與前置管柱之選擇對中藥製劑茵陳蒿湯各成分層析效果的影響在本文中均有詳盡的探討。

A high-performance liquid chromatographic method was developed to separate ten bioactive constituents in Artemisiae Capillaris Herba. The method was carried out by using a gradient solvent system of phosphate buffer-methanol-acetonitrile, Cosmosil 5Cis-MS column and detecting at 280 nm, and the contents in Artemisiae Capillaris Herba extract could easily be determined within 85 minutes. The relationship between the external appearance and the quality of the herb drug had been investigated, and the differences among the samples of Artemisiae Capillaris Herba and Origani Herba were also evaluated. The effect of column, mobile phase and precolumn on the separability was studied.
Yin-chen-hao-tang, a Chinese herbal preparation, is known to consist of Artemisiae Capillaris Herba, Gardeniae Fructus and Rhei Rhizoma. A high-performance liquid chromatographic method for sitmultaneous determination of five components in Artemisiae Capillaris Herba, three in Gardeniae Fructus and five in Rhei Rhizoma was developed. Detecting at 250 nm with a Cosmosil 5C18-MS column and a linear gradient elution system consisted of KH2PO4 methanol and acetonitrile was found to be able to determine thirteen constituents within 80 minutes. Using this method, various samples of Yin-chen-hao-tang were evaluated and compared. The effect of column, mobile phase and precolumn on separation was also studied.