本研究觀察到在蕃茄果實中除了polygalacturonase及pectin methylesterase兩個主要表現的酵素之外，另有一分子量15 kDa的蛋白質其表現量亦極為顯著。由於該蛋白質的生物功能至今不詳，在此將其命名為Tomato fruit 15-kDa protein，並簡稱為TF15蛋白質。
TF15蛋白質普遍存在於各不同品系的蕃茄果實內，且於各不同發育時期皆有表現。為了選殖TF15蛋白質的生合成基因，本研究製備了蕃茄果實的cDNA library，並藉專一抗體從中選殖出HL4及HL5兩個cDNA clones。其中HL5全長為740 bp，內含一個333 bp的完整讀序，並可轉譯出110個氨基酸。TF15蛋白質的氨基酸序列中以His、Glu、Lys等三個氨基酸的含量最為豐富，分別佔全部氨基酸的19.09 %、17.27 % 及16.36 %。在該蛋白質中親水性氨基酸與疏水性氨基酸以短片段長度重複鑲嵌，形成a-helix的二級結構。在基因庫內進行比對的結果，發現TF15蛋白質與前人報導的ASR蛋白質 (Iusem et al., 1993) 有顯著的相似性。
相較於植株的根、莖、葉片等器官，TF15蛋白質在蕃茄植株中主要表現於果實部位。此外，TF15蛋白質也會受到PEG滲透逆境或ABA的處理而有短暫性之輕微誘導表現。蕃茄果實在以1 % Ethephon進行人工催熟時，TF15蛋白質的表現量並不隨著Ethephon處理時間的增加而有所改變。且無論是對乙烯不敏感的Never Ripe突變種或是對乙烯敏感的亞蔬CL5915-93D4-1-0-3品種，其果實內TF15蛋白質的表現量均為一致，故推論TF15蛋白質的表現不受荷爾蒙乙烯的影響。此外，雖然TF15蛋白質之基因表現量會隨著果實的成熟而有逐漸增加的趨勢，但其蛋白質表現量卻在果實發育至MG時期後，即已臻穩定狀態，此一結果顯示在蕃茄果實中，TF15蛋白質的生合成基因之表現，可能在post-transcriptional的層次上具有調控的機制。

In this study, we observed in tomato fruit the presence of a predominant 15-kDa protein, in addition to polygalacturonase and pectin methylesterase. Since the biological function of this protein is not yet known, here we named it as tomato fruit 15-kDa protein (TF15 protein).
TF15 protein can be detected in tomato fruits from various cultivars and of different developmental stages. To identify its encoding gene, we have constructed a tomato fruit cDNA library and selected from it two positive clones, HL4 and HL5, using an antiserum directed to the TF15 protein. The HL5 clone is 740 base pairs in length and contains an open reading frame encoding a polypeptide of 110 amino acids. The translated polypeptide is rich in His (19%), Glu (17%), and Lys (16%). Beside, the hydrophilic and hydrophobic amino acids within this polypeptide are present as intervened segments, suggesting the existence of an a-helix secondary structure. The result of a protein database search indicates the TF15 protein is high homologous to the ASR protein (Iusem et al., 1993).
Comparative to its levels in the root, stem, and leaves of tomato plant, TF15 protein is mainly expressed in the fruit. In the stem, however, the expression of TF15 protein may be transiently and slightly induced by PEG or ABA treatment. While Ethephon treatment lead tomato fruits of mature-green stage to ripened, the level of TF15 protein in these fruits remained unchanged. In addition, the levels of TF15 protein in both ethylene-sensitive (AVDRC CL5915-93D4-1-0-3) and ethylene-insensitive (Never ripe) tomato fruits were indistinguishable. These results indicate the expression of TF15 protein is not ethylene-stimulated. In ripening tomato fruits, the expression patterns of TF15 protein and its RNA transcript are distinct. For the former, the protein level has reached a steady state when fruits are at mature-green stage; while for the latter, the RNA level remains increasing along the ripening process. These data suggest the expression of TF15 protein and its encoding gene may be regulated at the post-transcriptinal level.

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