高效能液相層析 (HPLC) 及毛細管電泳 (CE) 是目前最常用來定量中藥指標成分的分析方法。本研究以這兩種儀器，開發芍藥藥材、芍藥黃芩藥對及芍藥湯製劑之分析方法，並用環烯醚帖類化合物群的分析數據，比較 CE 及 HPLC 分析方法之優劣。
結合膠束電動力學毛細管層析 (MEKC) 及毛細管區帶電泳 (CZE)兩分析技術可成功地分離中藥材芍藥中的八個指標成分。CZE 方法可定量 paeonol，oxypaeoniflorin，benzoic acid，pentagalloylglucose和 gallic acid；在 MFKC 方法中添加 sodium cholate 可分析albiflorin，paeoniflorin，Denzoylalbiflorin，oxypaeoniflorin 和paeonol。濃度的線性範圍可達兩個數量級；各成分的偵測極限範圍在 2.6-23.7μg/ml 之間。移動時間的相對標準偏差低於 1.43% (n=6)。本分析方法可直接定量芍藥材的乙醇-水萃取液中的八個指標成分。
芍藥黃芩藥對中，同時含有芍藥的配醣體化合物及黃芩的黃酮類成分。本研究以逆相 HPLC 分析方法，沖提系統之流動相(A)為20mM MH2PO4 以 0.01﹪H3PO4 調至 pH=4.15，流動相(B)為H2O/CH3CN=20/80 (V/V)，利用 Cosmosile 5C18-MS 之分離管柱，以230及273nm為偵測波長，可在60分鐘內，成功地分離藥對萃取液中的十四個指標成分，包括芍藥的八個指標成分及黃芩的baicalin，wogonin 7-O-glucuronide，oroxylin A 7-O-glucuronide，baicalein，wogonin 和 oroxylin A 共六個成分。本方法亦可運用於其他相關製劑之定量，例如：柴胡桂枝湯，柴葛解肌湯，小續命湯，黃芩湯，荊芥連翹湯及五淋散。
為比較 CE 與 HPLC 兩方法之優劣，並了解化合物結構與分析方法之相關性，本研究分別開發九種環烯醚帖類化合物，包括gardenoside、geniposide、geniposidic acid、shanzhiside、loganin、loganic acid、aucubin、harpagoside、catalpol 之 CE 與 HPLC 分析方法。在 CE 部分，採 CZE 分離模式，利用 50mM Na2B4O7 及30mg/ml 2,6-di-O-methyl-β-cyclodextrin 緩衝溶液，在32分鐘內可分析八種環烯醚帖類化合物。在 HPLC 部分，利用磷酸鹽及氰甲烷沖提系統可在45分鐘內，分離九種化合物。結果顯示兩方法在分析時間、基線穩定性、化合物移動順序及各化合物之理論板數等方面有明顯的差異。

The methods to determine Chinese herbs are usually performed by capillary electrophoresis (CE) and high-performance liquid chromatography (HPLC). Both of these methods were used in this study to assay the constituents of Paeoniae Radix, the constituents of the peony-scute herb couple, and nine iridoids. Besides developing the analytical methods for Chinese herbs, the goals of this study are to compare the differences of CE and HPLC and to establish the relationship of analyses and analytical conditions.
A method combining the techniques of capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC) has been developed to separate a total of eight peony constituents. The CZE method was used to determine the contents of paeonol, oxypaeoniflorin, benzoic acid, pentagalloylglucose and gallic acid, and the MEKC technique based on sodium cholate was applied to analyze albiflorin, paeoniflorin, benzoylalbiflorin, paeonol and oxypaeoniflorin. Linearity around two orders of magnitude of concentration was generally obtained and limits of detection for these compounds were in the range of 2.6-23.7 μg/mL. The relative standard deviations of migration times were less than 1.43% (n=6). Contents of peony constituents in an ethanol-water extract of Paeonia lactiflora Pall. sample could easily be determined by this method.
A high-performance liquid chromatography method was developed to separate paeonol, oxypaeoniflorin, benzoic acid, pentagalloylglucose, albiflorin, paeoniflorin, benzoylalbiflorin, gallic acid, baicalin, wogonin 7-O-glucuronide, oroxylin A 7-O-glucuronide, baicalein, wogonin and oroxylin A in the peony-scute herb couple. Detection at 230 nm and 275 nm with a linear gradient elution system consisted KH2P04 and acetonitrile was found to be able to determine fourteen constituents within 60 min. This method can also to be applied to analyse a number of Chinese herbal preparations such as Chai-hu-kuei-chih-tang, Chai-ko-chieh-chi-tang, Hsiao-hsu-ming-tang, Huang-chin-tang, Ching-chieh-lien-chiao-tang, and Wu-lin-sang.
In order to compare the superiority and shortcoming of both methods and to understand the relationship between chemical structures and analysis methods, this study has developed both CE and HPLC methods for the analysis of nine iridoids including gardenoside, geniposide, geniposidic acid, shanzhiside, loganin, loganic acid, aucubin, harpagoside and catapol. The CE method made use of CZE mode of separation with 50 mM Na2B4O7 and 30 mg/ml 2,6-di-O-methyl-β- cyclodextrin as a buffer for the determination of eight iridoids within 32 minutes. The HPLC method used phosphates and acetonitrile as an elution system to separate and determine nine compounds within 45 minutes. The results showed very marked differences between the two methods in terms of the analysis time, base-line stability, chemical compound migration sequence and the number of theoretical plates of the various compounds.