在植物演化過程中，葉綠體將大多數基因已經轉送至植物細胞核內。因此葉綠體發育及執行正常功能皆受到細胞核基因調控。之前研究發現CIA2是一個轉錄因子，負責調節轉運蛋白機組基因atToc75和atToc33的表現，及調控葉綠體輸入蛋白質的效率。並由阿拉伯芥基因微矩陣晶片調查發現CIA2調節葉綠體分子伴侶蛋白CPN10以及葉綠體核糖體蛋白基因表現。為了要了解CIA2是否在植物細胞內直接調節這些下游基因表現，我們利用染色質免疫沈澱實驗來探討CIA2與否直接鍵結在下游基因啟動子上。實驗結果顯示，CIA2直接參與調控atToc33、atToc75-III、CPN10，及部分葉綠體核糖體蛋白RPL18、RPS6、及RPL15基因。而我們也利用生物資訊工具調查CIA2下游atToc33、atToc75-III、CPN10及葉綠體核糖體蛋白基因啟動子，結果發現兩個未知的順式作用元素CIARE1及CIARE2。由膠體電泳位移分析發現CIARE1 (CIA2-regulatory element)及CIARE2皆會與蛋白質結合。因此，染色質免疫沈澱實驗及膠體電泳位移分析結果進一步釐清CIA2參與調控下游基因的過程，並且幫助了解CIA2參與調控葉綠體發育相關之基因表現的途徑。

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