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Author: 李亞珊
Lee, Ya-Shan
Thesis Title: 銀離子對廣鹽性青鱂魚胚胎離子調節與海水適應能力之影響
Effects of silver ion on ion regulation and seawater adaptation in euryhaline medaka (Oryzias latipes) embryos
Advisor: 林豊益
Lin, Li-Yih
Committee: 洪君琳
Horng, Jiun-Lin
周銘翊
Chou, Ming-Yi
林豊益
Lin, Li-Yih
Approval Date: 2022/01/18
Degree: 碩士
Master
Department: 生命科學系
Department of Life Science
Thesis Publication Year: 2022
Academic Year: 110
Language: 中文
Number of pages: 61
Keywords (in Chinese): 銀離子青鱂魚離子細胞離子調節滲透壓調節鹽度挑戰
Keywords (in English): silver ion, medaka, ionocyte, ionregulation, osmoregulation, salinity challenge
Research Methods: 實驗設計法
DOI URL: http://doi.org/10.6345/NTNU202200322
Thesis Type: Academic thesis/ dissertation
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  • 摘要 vi Abstract vii 研究背景 1 奈米銀的汙染與毒性研究 1 銀離子的毒性研究 2 鹽度對銀離子毒性的影響 3 青鱂魚模式動物 4 魚類的離子細胞(Ionocyte)與滲透壓調節 5 研究目的 7 實驗流程 8 一、比較銀離子在淡水與海水的毒性 8 二、銀離子對青鱂魚胚胎腸道的影響 8 三、銀離子對青鱂魚胚胎的海水適應能力之影響 8 實驗設計 9 實驗1:比較淡水與海水環境中以硝酸銀處理7天後青鱂魚胚胎之累積死亡率與心跳速率。 9 實驗2:比較淡水與海水環境中以硝酸銀處理7天對青鱂魚胚胎形態之影響。 9 實驗3:比較淡水與海水環境中以硝酸銀處理7天後對青鱂魚胚胎卵黃囊上離子細胞密度之影響。 9 實驗4:建立測量胚胎喝水量之方法。 10 實驗5:海水環境中以硝酸銀處理8天後對青鱂魚胚胎的相對喝水量的影響。 10 實驗6:海水環境中以硝酸銀處理7天後對青鱂魚胚胎腸道細胞的凋亡程度。 10 實驗7:海水環境中以硝酸銀處理8天後對青鱂魚胚胎腸道組織與細胞之影響。 11 實驗8:在淡水中以硝酸銀處理7天並轉移至海水後的青鱂魚胚胎之累積死亡率與心跳速率。 11 實驗9:在淡水中以硝酸銀處理7天並轉移至海水後的青鱂魚胚胎之形態。 11 實驗10:在淡水中以硝酸銀處理7天並轉移至海水後的青鱂魚胚胎之卵黃囊上離子細胞的密度與粒線體活性。 12 實驗11:比較以不同濃度硝酸銀處理的青鱂魚胚胎在海水轉移前後之卵黃囊上離子細胞頂膜開口密度與大小。 12 實驗12:在淡水中以硝酸銀處理7天並轉移至海水後的青鱂魚胚胎之相對喝水量。 12 實驗13:在淡水中以硝酸銀處理7天並轉移至海水後的青鱂魚胚胎之體內鈉離子含量。 13 材料與方法 14 實驗動物 14 硝酸銀溶液配置與實驗處理 14 心跳、體長、卵黃囊大小、眼睛大小、圍心腔大小、體軸彎曲程度與體軸面積的計算 15 免疫細胞螢光染色(Whole-mount immunocytochemistry, ICC) 15 具粒線體活性離子細胞的標定 17 相對喝水量的測量 17 組織包埋與蘇木素–伊紅染色(Hematoxylin and eosin stain, H&E stain) 19 穿透式電子顯微鏡(Transmission electron microscope, TEM) 20 掃描式電子顯微鏡(Scanning electron microscope, SEM) 20 電感耦合電漿體質譜法(Inductively coupled plasma mass spectrometry, ICP-MS) 20 數據統計方法 21 結果 22 實驗1:比較淡水與海水環境中以硝酸銀處理7天後青鱂魚胚胎之累積死亡率與心跳速率。 22 實驗2:比較淡水與海水環境中以硝酸銀處理7天對青鱂魚胚胎形態之影響。 22 實驗3:比較淡水與海水環境中以硝酸銀處理7天後對青鱂魚胚胎卵黃囊上離子細胞密度之影響。 23 實驗4:建立測量胚胎喝水量之方法。 23 實驗5:海水環境中以硝酸銀處理8天後對青鱂魚胚胎的相對喝水量的影響。 24 實驗6:海水環境中以硝酸銀處理7天後對青鱂魚胚胎腸道細胞的凋亡程度。 24 實驗7:海水環境中以硝酸銀處理8天後對青鱂魚胚胎腸道組織與細胞之影響。 25 實驗8:在淡水中以硝酸銀處理7天並轉移至海水後的青鱂魚胚胎之累積死亡率與心跳速率。 25 實驗9:在淡水中以硝酸銀處理7天並轉移至海水後的青鱂魚胚胎之形態。 26 實驗10:在淡水中以硝酸銀處理7天並轉移至海水後的青鱂魚胚胎之卵黃囊上離子細胞的密度與粒線體活性。 26 實驗11:比較以不同濃度硝酸銀處理的青鱂魚胚胎在海水轉移前後之卵黃囊上離子細胞頂膜開口密度與大小。 27 實驗12:在淡水中以硝酸銀處理7天並轉移至海水後的青鱂魚胚胎之相對喝水量。 28 實驗13:在淡水中以硝酸銀處理7天並轉移至海水後的青鱂魚胚胎之體內鈉離子含量。 28 討論 29 銀離子在不同鹽度環境對青鱂魚胚胎的毒性差異 29 銀離子在海水環境中造成青鱂魚胚胎的腸道損傷 30 銀離子降低青鱂魚胚胎的海水適應能力 31 葡聚醣(Dextran)在動物實驗之應用 33 青鱂魚胚胎的喝水行為 34 魚類海水適應能力在環境毒理研究中的重要性 35 結論 37 參考文獻 38

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