自然界中許多生物體藉著多價反應機制來控制細胞表面受器與醣類分子間的辨識，這項機制為目前廣泛討論的課題。我們以醣類包覆金奈米粒子，研究其合成、性質及生物系統上的應用。此種金奈米粒子不僅擁有優異的水溶性，並且穩定存在各種離子濃度及酸鹼值的緩衝溶液中。我們首先以修飾甘露糖的金奈米粒子在電子顯微鏡下標定大腸桿菌特定的黏附蛋白，以及利用表面電漿共振光譜儀來偵測甘露糖、葡萄糖和半乳糖修飾之金奈米粒子與洋刀豆血球凝集素間的親合力及動力學解離常數。我們在此篇論文探討：以修飾醣分子的金奈米粒子做為高特異性之標定探針，及生物系統中多價鍵反應的優秀載體。

Multivalent interactions between cell surface receptors and carbohydrates have been discovered in a number of biological processes. The synthesis, characterization and biological application of carbohydrate encapsulated gold nanoparticles (C-AuNP) are reported. C-AuNP is well dispersed and very stable without aggregation in the media of broad ion strength and pH ranges. We have demonstrated that mannose-encapsulated gold nanoparticles (m-AuNP) can be used as a probe to target mannose adhesin FimH of living bacterial type 1 pili using transmission electron microscopy, and explore the multivalent interactions between Con A and mannose-, glucose- and galactose-encapsulated gold nanoparticles studied using surface plasmon resonance (SPR). This work demonstrates that carbohydrate attached nanoparticles can be used as an efficient affinity label and a good a multi-ligand carrier in a biological system.

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