韻律蛋白COP23（Circadian Oscillating Protein, 23 kDa）是位於聚球藻RF-1細胞膜上的蛋白質，其mRNA的轉錄以及蛋白質的合成、含量變化與分解，均具有概日韻律現象。本論文研究分兩部分：（一）探討不同光質與COP23概日韻律的建立和表現的關係。將聚球藻RF-1細胞分別以12小時光照（白、紅或藍光）、12小時黑暗的光暗週期馴化一段時間後，改置於連續光照（free running time）下的第25小時開始，每4小時取樣一次，利用SDS-PAGE及西方轉漬法（western blotting）分析COP23的表現，實驗結果顯示，不論是白、紅或藍光均可建立其概日韻律。若以短暫的遠紅光打斷馴化過程中之光週期時，其韻律週期仍舊存在，由此推測聚球藻RF-1之COP23概日韻律的建立與表現與光敏素無關，應有其他的光接受器（photoreceptors）參與其訊息傳遞路徑。（二）建立聚球藻RF-1的轉殖系統。本研究嘗試利用自然轉型法及電穿孔法將質體送入聚球藻RF-1中，以建立聚球藻RF-1的轉殖系統，但未成功。

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