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研究生： 張智勝
Chih-Sheng, Jhang
論文名稱： 利用紙片電噴灑質譜快速篩選及毛細管電泳質譜測定唾液中含4-氯安非他命的開發與研究
Rapid screening and determination of 4-chloroamphtamine in saliva by paper spray-mass spectrometry and capillary electrophoresis-mass spectrometry
指導教授： 林震煌
Lin, Cheng-Huang
學位類別： 碩士
Master
系所名稱： 化學系
Department of Chemistry
論文出版年： 2012
畢業學年度： 100
語文別： 中文
論文頁數： 71
中文關鍵詞： 紙片電噴灑質譜 、毛細管電泳電噴灑質譜 、4-氯安非他命
英文關鍵詞： paper spray-mass spectrometry, capillary electrophoresis electrospray ionization-mass spectrometry, 4-chloro-amphetamine
論文種類： 學術論文
相關次數： 點閱：276  下載：8 
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    • 本實驗開發了一種新型藥物篩選系統，包括濾紙電噴霧質譜（PS-MS）和毛細管電泳電噴灑質譜法（CE-ESI-MS）的方法。本實驗系統可以很容易地切換的PS-MS快速篩選樣品或傳統CE-ESI-MS分離方法，並利用同時共享同一台質譜而獲得詳細的質譜信息。在PS-MS情況下，當用尖端為（15度）的濾紙時，從紙尖到質譜入口的最佳距離大約口為7.7 mm，而最佳化CE-ESI尖端的距離為13.5 mm。另外本實驗是使用4 -氯安非他命作為測試樣品，而PS-MS和CE-ESI-MS偵測極限經過測試後確定分別為0.1和0.25 ppm。以下是使用PS-MS和CE-ESI-MS和實驗條件下得到的結果的比較與說明。

    A novel drug-screening system, consisting of paper spray-mass spectrometry (PS-MS) and a capillary electrophoresis electrospray ionization-mass spectrometry (CE-ESI-MS) method was developed. This system can be easily switched either to PS-MS for rapidly screening samples or to the traditional CE-ESI-MS method for separation and to obtain detailed mass spectral information, while sharing the same mass spectrometer. In the former case, when a sharp (15º-tip) chromatography paper was used, the optimized distance from the paper tip to the mass inlet was 7.7 mm, whereas the optimized distance for the CE-ESI tip was ~ 13.5 mm. Using 4-chloro-amphetamine as a model compound, the limits of detection for PS-MS and CE-ESI-MS were determined to ~ 0.1 and 0.25 ppm, respectively. Comparisons of results obtained using PS-MS and CE-ESI-MS and the experimental conditions are described.

    中文摘要....................................................Ⅰ 英文摘要....................................................Ⅱ 目錄.......................................................Ⅲ 圖目錄.....................................................Ⅴ 表目錄.....................................................Ⅵ 第一章、緒論................................................1 1-1 研究目的.............................................1 1-2 分析物簡介...........................................2 1-2-1 鹵素安非他命結構圖..............................3 1-3 濫用藥物的檢驗技術.....................................4 1-4 生物檢體之檢驗分析.....................................8 第二章、分析方法與原理.......................................10 2-1 毛細管電泳電噴灑質譜法 (CE-ESI-MS).....................10 2-2 離子阱質量分析器簡介...................................12 2-3 紙片電噴灑質譜法 (PS-MS)..............................13 2-4 大氣壓基質輔助雷射脫附游離法 (AP-MALDI).................14 2-5 電噴灑輔助雷射脫附游離法 (ELDI)........................15 2-6 萃取方法............................................16 第三章、儀器、藥品及實驗方法..................................18 3-1 CE-ESI-MS結合PS-MS之實驗方法.........................18 3-1-1 自組式毛細管電泳質譜法 (CE-ESI-MS)..............18 3-1-2 紙片電噴灑游離法 (PS-MS).......................21 3-2 常見雷射脫附游離之實驗方法..............................24 3-2-1 大氣壓基質輔助雷射脫附質譜 (AP-MALDI-MS).............24 3-2-2 電噴灑輔助雷射脫附質譜 (ELDI-MS)....................27 3-2-3 真空噴灑沉積 (spray deposition)...................30 3-3 儀器及周邊設備列表....................................31 3-4 藥品列表............................................32 第四章、結果與討論..........................................34 4-1 自製鋼筆頭式PS-MS及CE-ESI-MS位置最佳化.................35 4-1-1 PS-MS位置校正圖..............................36 4-1-2 CE-ESI-MS位置校正圖..........................41 4-1-3 PS-MS及CE-ESI-MS最佳位置比較..................43 4-2 CE-ESI-MS分離鹵素安非他命............................45 4-3 鹵素安非他命之真實樣品應用.............................47 4-3-1 唾液中鹵素安非他命的偵測..............................47 4-3-2 真實樣品前處理......................................48 4-4 雷射脫附游離源偵測鹵素安非他命..........................51 4-5 各種游離源偵測鹵素安非他命之整理.........................63 第五章、結論...............................................65 參考文獻..................................................66

    [1] C. H. Frith, L. W. Chang, D. L. Lattin, R. C. Walls, J. Hamm, R. Doblin, Fundamental and Applied Toxicology 9 (1987) 110.
    [2] K. Pihlainen, K. Grigoras, S. Franssila, R. Ketola, T. Kotiaho, R. Kostiainen,
    J. Mass Spectrom. 40 (2005) 539.
    [3] S. L. Chong, T. Nissila, R. A. Ketola, S. Koutaniemi, M. Derba-Maceluch,
    E. J. Mellerowicz, M. Tenkanen, P. Tuomainen, Anal. Bioanal.Chem. 401 (2011) 2995.
    [4] M. Cui, M. A. McCooeye, C. Fraser, Z. Mester, Anal. Chem. 76 (2004) 7143.
    [5] K. Shrivas, H. F. Wu, Rapid Commun. Mass Spectrom.21 (2007) 3103.
    [6] M. Z. Huang, S. S. Jhang, C. N. Cheng, S. C. Cheng, J. Shiea, Analyst 135 (2010) 759.
    [7] I. X. Peng, R. R. O. Loo, E. Margalith, M. W. Little, J. A. Loo, Analyst 135 (2010) 767.
    [8] I. X. Peng, J. Shiea, R. R. O. Loo, J. A. Loo, Rapid Commun. Mass Spectrom.21 (2007), 2541.
    [9] S. C. Cheng, Y. S. Lin, M. Z. Huang, J. Shiea, Rapid Commun. Mass Spectrom.24 (2010) 203.
    [10] J. Liu, H. Wang, N. E. Manicke, J. M. Lin, R. G. Cooks, Z. Ouyang, Anal. Chem. 82 (2010) 2463.
    [11] Q. Yang, H. Wang, J. D. Maas, W. J. Chappell, N. E. Manicke, R. G. Cooks, Z. Ouyang, Int. J. Mass Spectrom. 312 (2012) 201.
    [12] Z. Zhang, W. Xu, N. E. Manicke, R. G. Cooks, Z.Ouyang, Anal. Chem. 84 (2012) 931.
    [13] N. E. Manicke, Q.Yang, H. Wang, S. Oradu, Z. Ouyang, R. G. Cooks, Int. J. Mass Spectrom. 300 (2011) 123.
    [14] B. Hu, P. K. So, H. Chen, Z. P. Yao, Anal.Chem. 83 (2011) 8201.
    [15] K. Huikko, P. Ostman, C. Sauber, F. Mandel, K. Grigoras, S. Franssila, T. Kotiaho, R. Kostiainen, Rapid Commun. Mass Spectrom. 17 (2003) 1339
    [16] P. K. Salo, H. Salomies, K. Harju, R. A. Ketola, T. Kotiaho, J. Yli-Kauhaluoma, R. Kostiainen, J. Am. Soc. Mass Spectrom. 16 (2005) 906.
    [17] H. F. Wu, C. H. Lin, Rapid Commun. Mass Spectrom.20 (2006) 2511.
    [18] J. M. Daniel, V. V. Laiko, V. M. Doroshenko, R. Zenobi, Anal. Bioanal.Chem. 383 (2005) 895.
    [19] T. Keough, M. P. Lacey, R. J. Strife, Rapid Commun. Mass Spectrom.15 (2001) 2227.
    [20] P. M. Remes, G. L. Glish, J. Am. Soc. Mass Spectrom. 20 (2009) 1801.
    [21] W. E. Steiner, B. H. Clowers, W. A. English, H. H. Hill, Rapid Commun. Mass Spectrom.18 (2004) 882.
    [22] S. Trimpin, E. D. Inutan, T. N. Herath, C. N. McEwen, Anal. Chem. 82 (2010) 11.
    [23] H. Wei, K. Nolkrantz, D. H. Powell, J. H. Woods, M. Ko, R. T. Kennedy, Rapid Commun. Mass Spectrom.18 (2004) 1193.
    [24] K. Shrivas, H. F. Wu, Anal. Chem. 80 (2008) 2583.
    [25] I. X. Peng, J. Shiea, R. R. O. Loo, J. A. Loo, Rapid Commun. Mass Spectrom.21 (2007) 2541.
    [26] J. Dong, Y. H. Rezenom, K. K. Murray, Rapid Commun. Mass Spectrom.21 (2007) 3995.
    [27] F. Musshoff, J. Trafkowski, U. Kuepper, B. Madea, J. Mass Spectrom. 41 (2006) 633.
    [28] M. ndersson, E. ustavsson, N. Stephanson, O. eck, J. Chromatogr B 861
    (2008) 22.
    [29] M. Concheiro, S. M. S. S. Simoes, O. Quintela, A. Castro, M. J. R. Dias, A. Cruz, M. Lopez-Rivadulla, Forensic Sci. Int. 171 (2007) 44.
    [30] Van Bocxlaer, J. F., Clauwaert, K. M., Lambert, W. E., Deforce, D. L., Van den Eeckhout, E. G., & De Leenheer, A. P. , Mass spectrum. Rev. 19 (2000) 165.
    [31] A. Numan, N. D. Danielson, Anal. Chim.Acta 460 (2002) 49.
    [32] E. Tanaka, T. Kanata, M. Katagi, H. Tsuchihashi, K. Honda, Forensic Sci. Int. 163 (2006) 152.
    [33] S. Laks, A. Pelander, E. Vuori, E. Ali-Tolppa, E. Sippola, I. Ojanpera, Anal. Chem. 76 (2004) 7375.
    [34] T. Kamata, M. Katagi, H. Tsuchihashi, Forensic Toxicol. 28 (2010) 1.
    [35] S. D. Brandt, S. Freeman, I. A. Fleet, P. McGagh, J. F. Alder, Analyst 130 (2005) 330.
    [36] UshtanaAntia, M.Sc., Malcolm D. Tingle, Ph.D., Bruce R. Russell, Ph.D., J Forensic Sci. 55 (2010) 1311.
    [37] Sleno L, Staack RF, Varesio E, Hopfgartner G, Rapid Commun. Mass Spectrom.21 (2007) 2301.
    [38] T. L. Chang, K. W. Chen, Y. D. Lee, K. Fan, J. Clin. Lab. Anal. 13 (1999) 106.
    [39] Simon Elliott, Bioanalysis 3 (2011) 249.
    [40] A. Ramseier, C. Siethoff, J. Caslavska, W. Thormann, Electrophoresis 21 (2000) 380.
    [41] M. Nieddu, G. Boatto, G. Dessi, J. Chromatogr. B 852 (2007) 578.
    [42] G. Boatto, M. Nieddu, A. Carta, A. Pau, M. Palomba, B. Asproni, R. Cerri, J. Chromatogr. B 814 (2005) 93.
    [43] Y. T. Iwata, T. Kanamori, Y. Ohmae, K. Tsujikawa, H. Inoue, T. Kishi, Electrophoresis 24 (2003) 1770.
    [44] M. Nieddu, G. Boatto, M. A. Pirisi, G. Dessi, Rapid Commun. Mass Spectrom.24 (2010) 2357.
    [45] R. Iio, S. Chinaka, N. Takayama, K. Hayakawa, J. Health Sci. 51 (2005) 693.
    [46] D. L. Lin, R. M. Yin, R. H. Liu, J. Food Drug Anal. 13 (2005) 193.
    [47] H. Fujii, K. Hara, M. Kageura, M. Kashiwagi, A. Matsusue, S. Kubo, Forensic Toxicology 27 (2009) 75.
    [48] A. A. Marais, J. B. Laurens, Forensic Sci. Int. 183 (2009) 78.
    [39] P. Meng, D. Zhu, H. He, Y. Wang, F. Guo, L. Zhang, Anal.Sci. 25 (2009) 1115.
    [50] Westphal F, Junge T, Girreser U, Stobbe S, Perez SB, Forensic Sci. Int. 187 (2009) 87.
    [51] Abdel-Hay KM, Awad T, DeRuiter J, Clark CR, Forensic Sci. Int. 195 (2010) 78.
    [52] Hiroyuki Inoue, Yuko T. Iwata, TatsuyukiKanamori, Hajime Miyaguchi, Kenji Tsujikawa, Kenji Kuwayama, HiroeTsutsumi, MunehiroKatagi, Hitoshi Tsuchihashi, TohruKishi, Jpn. J. Sci. Tech. Iden. 9 (2004) 165.
    [53] M. Pellegrini, F. Rosati, R. Pacifici, P. Zuccaro, F. S. Romolo, A. Lopez, J. Chromatogr. B 769 (2002) 243.
    [54] A. Nakamoto, A. Namera, M. Nishida, M. Yashiki, T. Kuramoto, K. Kimura, Forensic Toxicol. 25 (2007) 1.
    [55] T. Kanamori, K. Kuwayama, K. Tsujikawa, H. Miyaguchi, Y. Iwata, H. Inoue, T. Kishi, J. Health Sci. 52 (2005) 425.
    [56] F. Centini, A. Masti, I. B. Comparini, Forensic Sci. Int. 83 (1996) 161.
    [57] J. M. Wilson, F. McGreoge, S. Smolinske, R. Meatherall, Forensic Sci. Int. 148 (2005) 31.
    [58] S. D. Brandt, D. Mansell, S. Freeman, I. A. Fleet, J. F. Alder, J. Pharm. Biomed. Anal.41 (2006) 872.
    [59] S. P. Vorce, J. H. Sklerov, J. Anal. Toxicol.28 (2004) 407.
    [60] B. Holmstedt, W. J. A. Vandenheuvel, W. L. Gardiner, E. C. Horning, Anal. Biochem.81 (1964) 51.
    [61] D. Hensley, J. T. Cody, J. Anal. Toxicol.235 (1999) 18.
    [62] C. Jurado, M. P. Gimenez, T. Soriano, M. Menendez, M. Repetto, J. Anal. Toxicol.24 (2000) 11.
    [63] J. L. Valentine, R. Middleton, J. Anal. Toxicol.24 (2000) 211.
    [64] Segura, J., Ventura, R., Jurado, C., J. Chromatogr. B Biomed Sci. Appl. 713 (1998) 61.
    [65] R. Kikura-Hanajiri, M. Hayashi, K. Saisho, Y. Goda, J. Chromatogr. B 825 (2005) 29.
    [66] M. Takahashi, M. Nagashima, J. Suzuki, T. Seto, I. Yasuda, T. Yoshida, J. Health Sci. 54 (2008) 89.
    [67] T. Kanamori, K. Kuwayama, K. Tsujikawa, H. Miyaguchi, Y. T. Iwata, H. Inoue, Xenobiotica 38 (2008) 1476.
    [68] T. Kamata, M. Katagi, H. Kamata, A. Miki, N. Shima, K. Zaitsu, M. Nishikawa, H. Tsuchihashi, J. Health Sci. 53 (2007) 585.
    [69] M. Gros, M. Petrovic, D. Barcelo, Talanta 70 (2006) 678.
    [70] J. K. Eng, A. L. McCormack, J. R. Yates, J. Am. Soc. Mass Spectrom. 5 (1994) 976.
    [71] S. Souverain, S. Rudaz, J. L. Veuthey, J. Pharm. Biomed. Anal.35 (2004) 913.
    [72] M. J. Bogusz, H. Hassan, E. Al-Enazi, Z. Ibrahim, M. Al-Tufail, J. Pharm. Biomed. Anal.41 (2006) 554.
    [73] P. Garcia, A. C. Paris, A. Leufroy, M. A. Popot, Y. Bonnaire, Biomed. Chromatogr.26 (2012) 534.
    [74] E. I. Miller, F. M. Wylie, J. S. Oliver, Journal of Analytical Toxicology 32 (2008) 457.
    [75] N. Zhang, K. L. Hoffman, W. Li, D. T. Rossi, J. Pharm. Biomed. Anal.22 (2000) 131.
    [76] S. Pedersen-Bjergaard, K. E. Rasmussen, J. Chromatogr. A 1184 (2008) 132.
    [77] S. Steinbormer, J. Henion, Anal. Chem. 71 (1999) 2340.
    [78] C. K. Lai, T. Lee, K. M. Au, Y. W. Chen, Clinical Chemistry 43 (1997) 312.
    [79] B. Dirion, F. Lanza, B. Sellergren, C. Chassaing, R. Venn, C. Berggren,Chromatographia 56 (2002) 237.
    [80] C. C. Tsai, J. T. Liu, Y. R. Shu, P. H. Chan, C. H. Lin, J. Chromatogr. A 1101 (2006) 319.
    [81] Y. Y. Yang, J. T. Liu, C. H. Lin, Electrophoresis 30 (2009) 1084
    [82] M. Yonamine, N. Tawil, R. L. D. Moreau, O. A. Silva, J. Chromatogr. B 789 (2003) 73.
    [83] P. J. Meng, Y. Y. Wang, Forensic Sci. Int. 197 (2010) 80.
    [84] C. F. Lin, J. T. Liu, C. H. Lin, Analytical sciences 25 (2009) 845.

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