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研究生：	林群倫 Chun-Lun Lin
論文名稱：	蛋白質與奈米鑽石之表面吸附作用 Interaction of Protein Attachment on Nanodiamond surface
指導教授：	林震煌 Lin, Cheng-Huang
學位類別：	碩士 Master
系所名稱：	化學系 Department of Chemistry
論文出版年：	2015
畢業學年度：	103
語文別：	英文
論文頁數：	65
中文關鍵詞：	奈米鑽石、蛋白質
英文關鍵詞：	Nanodiamond, Protein
DOI URL：	https://doi.org/10.6345/NTNU202205298
論文種類：	學術論文
相關次數：	點閱：173 下載：7
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本篇論文研究的目的為探討奈米鑽石表面與蛋白質的作用。本篇利用兩種具不同表面電荷的奈米鑽石作為對照，分別與四種不同的蛋白質反應，包括肌紅素，牛血清蛋白，Alpha-Lactalbumin和胰島素。藉由紫外光¬可見光譜儀，粒徑儀，基質輔助雷射解吸質譜儀的幫助，可以計算出奈米鑽石表面上蛋白質的吸附率，進而理解蛋白質吸附在奈米鑽石表面的情況。以兩種奈米鑽石和四種蛋白質組合產生的八種結果，可以被大略的分成三種模式，分別是平衡模式，錯合物模式和聚合物模式。平衡模式表示蛋白質在奈米鑽石表面的吸附是一種動態平衡，可以計算出吸附平衡常數，例子有肌紅素。錯合物模式表示蛋白質會比平衡模式更緊密的吸附在奈米鑽石表面，覆蓋整個表面為止，例子有牛血清蛋白。聚合物模式和錯合物模式相似，只是蛋白質不僅僅是覆蓋奈米鑽石的表面，會更進一步的在表面上聚合，形成一層大於一個蛋白質單位的外層。例子有胰島素。

關鍵字: 奈米鑽石，蛋白質

The objective of this thesis is to understand the effect of proteins on Nanodiamond. In this thesis two kinds of Nanodiamonds with different surface charge were used, reacting with four kinds of proteins respectively. Proteins used here are Myoglobin，Bovine serum albumin，Alpha-Lactalbumin and Insulin. By the assist of UV-Vis spectrometer, particle size analysis and MALDI, the surface coverage of proteins on Nanodiamonds can be calculated. Thus, the adsorption of Protein on Nanodiamonds can be observed.
Mixing two kinds of Nanodiamonds and four kinds of proteins gives eight kinds of results. These results can be briefly cataloged into three models, Equilibrium model, cluster model and polymer model. Equilibrium model means the status of protein adsorbed on the surface of Nanodiamond is a kinetic equilibrium. The equilibrium constant can be estimated and the affinity can be observed. The example is Myoglobin. Cluster model shows stronger adsorption of protein on Nanodiamond than Equilibrium model. The protein will adsorb on the Nanodiamond till the surface of Nanodaimond is used out. The example is Bovine serum albumin. Polymer model is similar to cluster model. The protein not only cover the surface of Nanodaimond, but form polymer with itself and form a shield on the Nanodiamond surface with more than one unit thick.

Key Words: Nanodiamond, Protein

Table of Contents……………………………………………………….I
摘要…………………………………………………………………….III
Abstract…………………………………………………………………IV
List of figures…………………………………………………………..VI
List of tables……………………………………………………………VII

Chapter 1 Introduction…………………………………………………..1
Chapter 2 Materials and Instruments……………………………………5
 2-1 Nanodiamonds……………………………………………………..5
   2-1-1  High pressure high temperature Nanodiamonds (HPHT-ND)5
   2-1-2  Monodisperse single nanodiamond (jND)…………………..5
 2-2 Proteins……………………………………………………………..6
 2-3 Instruments…………………………………………………………7
Chapter 3 Experiment………………………………………………..…12
Chapter 4 Result and discussion………………………………………..16
   4-1 Myoglobin + NDs………………………………………………17
     4-1-1  Comparison between two kinds of nanodiamonds………17
     4-1-2  Evidence of the attachment………………………………23
   4-2 Insulin + NDs…………………………………………………...27
4-2-1  Comparison between two kinds of nanodiamonds………27
     4-2-2  Evidence of the attachment………………………………36
   4-3 BSA +NDs………………………………………………….......38
4-3-1  Comparison between two kinds of nanodiamonds………38

4-4     Desorption of Protein-ND tested with DLS……………………43
     4-4-1  BSA+hND………………………………………………..49
     4-4-2  INS+hND………………………………………………...50
     4-4-3  Myoglobin+hND………….…….......................................51
     4-4-4  INS+jND…………………………………………………53
     4-4-5  BSA+jND………………………………………………..56
     4-4-6  Myoglobin+jND…………………………………………57
Chapter 5 Conclusion…………………………………………...............61
Reference………………………………………………………………..63
                                

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