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研究生: 范揚錦
Yang-Ching, Fan
論文名稱: 台灣桃園縣地區網柱細胞黏菌之調查
A survey of dictyostelid cellular slime molds in Taoyuan County, Taiwan
指導教授: 葉增勇
Yeh, Zeng-Yung
學位類別: 碩士
Master
系所名稱: 生命科學系
Department of Life Science
論文出版年: 2002
畢業學年度: 90
語文別: 中文
論文頁數: 67
中文關鍵詞: 細胞黏菌網柱細胞黏菌輪生細胞黏菌桃園台灣
英文關鍵詞: Dictyostelid cellular slime molds, Dictyostelium, Polysphondylium, Taoyuan, Taiwan
論文種類: 學術論文
相關次數: 點閱:440下載:22
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  • 民國88年7月至90年8月間,自桃園縣達觀山、小烏來、石門水庫、虎頭山及大棟山等不同地區之森林植被下土壤表層採取土壤樣品,分離出細胞黏菌純培養菌株,經鑑定後共得1科2屬10種,分別為巨大頭網柱細胞黏菌(Dictyostelium macrocephalum Hagiwara, Yeh et Chien)、布列菲氏網柱細胞黏菌(D. brefeldianum Hagiwara)、紫色網柱細胞黏菌(D. purpureum Olive)、根足網柱細胞黏菌(D. rhizopodium Raper et Fennell)、纖細網柱細胞黏菌(D. delicatum Hagiwara)、淡紫柄網柱細胞黏菌(D. coeruleo-stipes Raper et Fennell)、細小網柱細胞黏菌(D. minutum Raper)、棍棒頭網柱細胞黏菌(D. clavatum Hagiwara)、透明輪生細胞黏菌(Polysphondylium pallidum Olive)和紫色輪生細胞黏菌(P. violaceum Brefeld)。其中淡紫柄網柱細胞黏菌、細小網柱細胞黏菌及棍棒頭網柱細胞黏菌,皆為桃園地區分離出之台灣新紀錄種。透明輪生細胞黏菌及紫色網柱細胞黏菌出現的頻率最高,是桃園地區廣泛分布的種類。
    文中除記述各種細胞黏菌形態特徵及附圖說明,並製作檢索表。保存之菌種可供作中學鄉土生物輔助教材用。

    Samples were collected under forest soils from Ta-kuan-shan, Hsio-wu-lai, Shih-men Reservoir, Hu-tou-shan and Ta-tung-shan areas in Taoyuan County, during July 1999 to Auguest 2001. Some isolates of dictyostelid cellular slime molds were obtained. They were identified as one family, two genera, and ten species, namely: Dictyostelium macrocephalum Hagiwara, Yeh et Chien, D. brefeldianum Hagiwara, D. purpureum Olive, D. rhizopodium Raper et Fennell, D. delicatum Hagiwara, D. coeruleo-stipes Raper et Fennell, D. minutum Raper, D. clavatum Hagiwara, Polysphondylium pallidum Olive and P. violaceum Brefeld. Among them, D. coeruleo-stipes Raper et Fennell, D. minutum Raper and D. clavatum Hagiwara were reported as new records to Taiwan.
    The distribution and frequency of occurrence of these ten species were also determined. P. pallidum Olive and D. purpureum Olive are widely distributed in Taoyuan County.
    These species were described and illustrated in this text. Pure cultures are maintained and can be used as biological teaching materials for the middle high schools in Taiwan.

    摘要…………………………………………………………IV 英文摘要…………………………………………………… V 前言………………………………………………………… 1 壹、細胞黏菌簡介………………………………………… 1 (一)細胞黏菌的發現…………………………………… 1 (二)細胞黏菌的生活史………………………………… 2 (三)細胞黏菌的分類地位……………………………… 10 (四)細胞黏菌的分布…………………………………… 12 貳、細胞黏菌的研究歷史………………………………… 14 (一)國外學者的研究…………………………………… 14 (二)台灣學者的研究…………………………………… 15 參、桃園縣環境概況……………………………………… 18 (一)達觀山自然保護區………………………………… 18 (二)小烏來風景特定區………………………………… 19 (三)石門水庫…………………………………………… 19 (四)虎頭山公園………………………………………… 20 (五)大棟山……………………………………………… 20 肆、研究目的……………………………………………… 20 材料與方法………………………………………………… 22 壹、採樣…………………………………………………… 22 貳、培養基………………………………………………… 23 參、分離與純培養………………………………………… 23 肆、形態觀察……………………………………………… 24 伍、菌種的鑑定…………………………………………… 24 陸、細胞黏菌出現頻率分析……………………………… 25 柒、菌種的保存…………………………………………… 25 結果………………………………………………………… 26 壹、檢索表………………………………………………… 27 (一)屬的檢索表………………………………………… 27 (二)種的檢索表………………………………………… 27 貳、種的描述……………………………………………… 29 (1)巨大頭網柱細胞黏菌………………………………… 29 (2)布列菲氏網柱細胞黏菌……………………………… 29 (3)紫色網柱細胞黏菌…………………………………… 30 (4)根足網柱細胞黏菌…………………………………… 31 (5)纖細網柱細胞黏菌…………………………………… 32 (6)淡紫柄網柱細胞黏菌………………………………… 33 (7)細小網柱細胞黏菌…………………………………… 34 (8)棍棒頭網柱細胞黏菌………………………………… 35 (9)透明輪生細胞黏菌…………………………………… 36 (10)紫色輪生細胞黏菌……………………………………37 參、網柱細胞黏菌圖版………………………………………38 肆、出現頻率分析……………………………………………48 討論……………………………………………………………51 結論……………………………………………………………56 參考文獻………………………………………………………57 附錄:細胞黏菌的形態特徵…………………………………66

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