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| 研究生： |
許齊文 Chi-Wen Shiu |
|---|---|
| 論文名稱： |
磁減量生化檢測應用於 人類肝癌及大腸癌之檢驗特性研究 Immunomagnetic rednction of human Alpha-fetoprotein and carcinoembryonic Antigen |
| 指導教授： |
洪姮娥
Horng, Herng-Er 謝振傑 Chieh, Jen-Je |
| 學位類別： |
碩士 Master |
| 系所名稱： |
光電工程研究所 Graduate Institute of Electro-Optical Engineering |
| 論文出版年： | 2012 |
| 畢業學年度： | 100 |
| 語文別： | 中文 |
| 論文頁數： | 38 |
| 中文關鍵詞： | 磁減量 |
| 論文種類： | 學術論文 |
| 相關次數： | 點閱：72 下載：0 |
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在臨床診斷上約 60%~70% 的肝癌患者血清中Alpha-fetoprotein ( AFP ) 濃度較正常人來的高 ( AFP cut-off 值為 20 mg/ml )，適合作為篩檢肝癌的腫瘤指標蛋白 ( biomarker )。另外在臨床診斷上約50%~80%的大腸癌患者血清中Carcinoembryonic Antigen ( CEA ) 濃度較正常人來的高 ( CEA cut-off 值為 5 ng/ml )，適合作為篩檢大腸癌的 biomarker。然而現行普遍使用的免疫檢測如酵素連結免疫吸附分析 ( Enzyme-Link Immunosorbent Assay；ELISA ) 因其操作過程繁雜與易受待測樣品影響等種種問題，我們需要另尋一種更快速且準確的免疫檢測方法。
本實驗室已歷經使用Anti-CRP( C-reactive protein )與奈米磁性粒子結合，形成一具有磁性生物標記( magnetic biomarker ) 之磁性試劑( magnetic reagent )。再利用本實驗室所研發之磁減量檢測法( Immunomagnetic rednction ; IMR )，其靈敏度可達10-4 mg/ml，於是本研究中我們如法泡製將Anti-AFP 和Anti-CEA 分別與奈米磁性粒子結合成為AFP試劑和CEA試劑，並進行試劑長期穩定度觀察、干擾度測試，最後藉由IMR檢測人類血清檢體，以探討人類肝癌及大腸癌之檢驗特性。
參考文獻
1. 行政院衛生署國民健康局98年度癌症登記資料報告
2. C.Y. Hong, C.C. Wu, Y.C. Chiu, S.Y. Yang, H.E. Horng, and H.C. Yang, Appl. Phys. Lett. 88, 212512 (2006).
3. S.Y. Yang, Z.F. Jian, J.J. Chieh, H.E. Horng, H.C. Yang, and Chin-Yih Hong,” Wash-free, antibody-assisted magnetoreduction assays on orchid viruses”, J. Virol. Methods, 149, 334 (2008).
4. S.Y. Yang, J.J. Chieh, W.C. Wang, C.Y. Yu, C.B. Lan, J.H. Chen, H.E. Horng, Chin-Yih Hong, and H.C. Yang,” Ultra-highly sensitive and wash-free bio-detections for H5N1 virus via magnetoreduction assays”, J. Virol. Methods 153, 250 (2008).
5. M.W. Lu, S.Y. Yang, H.E. Horng, C.C. Yang, J.J. Chieh, Y.W. Hong, C.Y. Hong, H.C. Yang, and J.L. Wu,” Immunomagnetic reduction assay for nervous necrosis virus extracted from groupers”, IEEE J. Virol. Methods 181, 68 (2012).
6. Chen-Yu Chen, Chia-Chen Chang, Chun Yu, Shieh-Yueh Yang, and Chii-Wann Lin,” Immunomagnetic reduction for fetal fibronectin detection - A novel method to detect the preterm biomarker”, Biomed. Eng.: Appl. Basis. Commun., 23, 273 (2011).
7. H.E. Horng*, S.Y. Yang, C.M. Liu, P.S. Tsai, Chin-Yih Hong*, H.C. Yang, and C.C. Wu, “Bio-functionalized magnetic nanoparticles for immunomagnetic detections of human c-reactive protein”, Appl. Phys. Lett., 88, 252506 (3 pages) (2006).
8. M.J. Chiu, H.E. Horng, J.J. Chieh, S.H. Liao, C.H. Chen, B.Y. Shih, C.C. Yang, C.L. Lee, T.F. Chen, S.Y. Yang, C.Y. Hong, and H.C. Yang,” Multi-channel SQUID-based ultra-high-sensitivity in-vitro detections for bio-markers of Alzheimer's disease via immunomagnetic reduction”, IEEE Trans. Appl. Supercond., 21, 477 (2011).
9. K. W. Huang, S. Y. Yang, C. Y. Yu, J. J. Chieh, Che-Chuan Yang, Herng-Er Horng, Chin-Yih Hong, Hong-Chang Yang, and Chau-Chung Wu,” Exploration of the relationship between the tumor burden and the concentration of vascular endothelial growth factor in liver-cancer-bearing animals using immunomagnetic reduction assay”, J. Biomed. Nanotechnol. 7, 535 (2011).
10. S.Y. Yang, C.S. Ho, C.L. Lee, B.Y. Shih, H.E. Horng, Chin-Yih Hong, H.C. Yang,Y.H. Chung, J.C. Chen and T.C. Lin,” Immunomagnetic reduction assay on chloramphenicol extracted from shrimp”, Food Chemistry 131 (2012) 1021–1025.
11. R. A. Goldsby, T. J. Kindt, B. A. Osborne, J. Kuby, FREEMAN,Immunology, New York.
12. J. Bauer, J. Chromatography B: Biomedical Sciences and Applications, 722, 55(1999).
13. P.J. Fisher, M.J. Springett, A.B. Dietz, P.A. Bulur, and S. Vuk-Pavlovic, J. Immunological Methods, 262, 95(2002).
14. W.Q. Jiang, H.C. Yang, S.Y. Yang, H.E. Horng, J.C. Hung, Y.C. Chen, and Chin-Yih Hong, J. Magn. Magn. Mater., 283, 210(2004).
15. H. E. Horng, S. Y. Yang, Y. W. Huang, W. Q. Jiang, C.-Y. Hong, and H.C. Yang, IEEE Trans. Appl. Supercond., 15, 668(2005).
16. H.E. Horng, Chin-Yih Hong, S.Y. Yang, H.C. Yang, S.H. Liao, C.M. Liu, and C.C. Wu, J. Korean Phys. Soc., 48, 999(2006).
17. R. Kötitz, W. Weitschies, L. Trahms, W. Brewer, and W. Semmler, J. Magn. Magn. Mater. 194, 62 (1999).
18. H. C. Yang, S. Y. Yang, S. H. Liao, G. L. Fang, W. H. Huang, C. H. Liu, H. E. Horng, and Chin-Yih Hong, J. Appl. Phys. 99, 124701 (2006).
19. H.E. Horng, S.Y. Yang, Y.W. Huang, W.Q. Jiang, C.Y. Hong, and H.C. Yang, IEEE Trans. Appl. Supercond. 15, 668 (2005).
20. H.J. Krause, N. Wolters, Y. Zhang, A. Offenhäusser, P. Miethe, M.H.F. Meyer, M. Hartmann, and M. Keusgen, J. Magn. Magn. Mater. 311, 436 (2007).
21. T.Rheinländer , R. Kötitz, W. Weitschies,W. Semmler, Journal of Magnetism and Magnetic Materials, 219, 219(2000).
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