摘要
含鐵黏土修飾電極可有效催化發光胺(3-aminophthalhydrazide，簡稱luminol)與過氧化氫之化學發光反應。為能探討此反應中所涉及之含氧中間產物，本論文利用N-acetyl-3,7-dihydroxyphenoxazine(簡稱amplex red)進行偵測。Amplex red可與氫氧自由基反應，並產生resorufin。我們藉由resorufin之生成及其螢光光譜，發現含鐵黏土微粒可有效催化生成O2-.離子自由基與OH.自由基。由於此一結果，我們便利用黏土修飾電極、葡萄糖酵素與amplex red對葡萄糖進行定量分析。此外，實驗亦顯示：維生素K3可有效催化O2-.離子自由基與OH.自由基之生成。根據此一結果，我們成功利用維生素K3、Fe(II)離子、超過氧物轉化酵素(superoxide dismutase，簡稱SOD)對二氯丙酸鈉、氯丙醇與氯酚進行分解。

Abstract
In order to identify the reactive oxygen species generated in the luminol chemiluminescence reaction, we used N-acetyl-3,7-dihydroxyphenoxazine (denoted amplex red) as a probe. Amplex red can react with hydroxyl radical to produce resorufin. Resorufin is a highly fluorescing species. Its formation and the associated characteristic emission bands become a useful tool in diagnosis of the reactive oxygen species derived from H2O2. In this thesis we described the results during this characterization. Besides, we also found that vitamin K3 (denoted VK3) is a useful electrochemical catalyst. Cyclic voltammetry and emission spectroscopy suggested that VK3 might form 1:1 complexes with 3-chloropropanol and sodium 2,3-dichloropropionate. The equilibrium constants were estimated to be 1000 M-1 and 100 M-1, respectively, for the reaction of VK3 with 3-chloropropanol and sodium 2,3-dichloropropionate based on the electrochemical techniques. Based on the emission spectroscopic method, the equilibrium constants were slightly greater, about 104 M-1. The reduced VK3 was also found as an effective catalyst for the reduction of oxygen. By using superoxide dismutase (SOD) and amplex red as probes, we confirmed that superoxide anion radical and the derived reactive oxygen species could be produced in aerated solutions as VK3 was incorporated. According to the in-situ monitoring of the proton levels in these systems, the reactive oxygen species produced by the catalysis of VK3 in aerated solutions were evidenced to be able to cause degradation of these chlorinated compounds.

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