摘要
環境荷爾蒙因其具有類似生物體內荷爾蒙的功能，能抑制荷爾蒙的正常作用，進而改變生物體內免疫、神經和內分泌系統的正常運作，近年來逐漸受到重視。鄰苯二甲酸酯( Phthalate esters, PAEs )即為其中之一類。在各種塑膠可塑劑當中，鄰苯二甲酸酯是被應用最多且最廣的一種；在任意棄置塑膠廢棄物與環境化學的作用下，鄰苯二甲酸酯類化合物( PAEs )很容易就流進環境中並且造成污染。
本研究利用組裝的加速溶劑萃取設備與連線的界面，與高效率液相層析儀作連線，來進行土壤中十二種鄰苯二甲酸酯的萃取與分析研究。方法兼具加速溶劑萃取法省時、省溶劑的優點，與高效率液相層析儀適於分析低揮發性化合物的優點。萃取所需溶劑只要4-6 mL，分析一個土壤樣品(包含萃取、層析偵測)只要1小時左右。方法之偵測極限可達2 g/g ~ 5 g/g之範圍。實際應用在真實土壤的分析上，顯示本法對於真實土壤的分析同樣可行。
關鍵詞：鄰苯二甲酸酯、環境荷爾蒙、加速溶劑萃取、高效率液相
層析儀、土壤、塑膠可塑劑

Abstract
One class of organic pollutants which has been extensively studied for their levels in the environment is phthalate esters. They are one of the most common plasticizers employed in the plastics industry. Carcinogenicity, hepatoxicity, and mutagenicity have been related with phthalate esters. Among the twelve target analytes studied in the present work, six have been listed by United State Environmental Protection Agency as top priority pollutants, and six have been listed by Environment Agency Government of Japan as endocrine disrupting compounds.
A method was developed in this study to couple an accelerated solvent extraction system (ASE) with high performance liquid chromatography (HPLC) for the analysis of PAEs in soil samples. Total solvent consumption for one extraction is only 4~6 mL, and total time consumption for a sample analysis is only about 1 hour. Detection limits for the PAEs in soil are ranged from 2 g/g ~ 5 g/g. Real samples collected at different sites in Taiwan have also been analyzed. The data shows the feasibly of this method for analyzing soil samples with satisfactory accuracy and precision.
Key word: endocrine disrupting compound ( EDC ), phthalate ester ( PAE ), accelerated solvent extraction ( ASE ), high performance liquid chromatography ( HPLC ), soil, plasticizer

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