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研究生: 王偉丞
Wei Cheng Wang
論文名稱: 磁減量檢測於豬流感病毒檢驗特性研究
指導教授: 洪姮娥
Horng, Herng-Er
楊謝樂
Yang, Shieh-Yueh
學位類別: 碩士
Master
系所名稱: 光電工程研究所
Graduate Institute of Electro-Optical Engineering
論文出版年: 2008
畢業學年度: 96
語文別: 中文
論文頁數: 41
中文關鍵詞: 磁性奈米粒子豬流行性感冒病毒生物標記
論文種類: 學術論文
相關次數: 點閱:240下載:0
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  • 本研究係利用化學共沉方法,合成出具有超順磁特性的磁性奈米粒子。因被介面活性劑包覆著,使磁性奈米粒子可在水溶液中均勻分佈。並進一步將豬流行性感冒病毒( Swine Influenza Virus )之抗體與磁性奈米粒子上結合,形成一具有磁性生物標記( magnetic bio-marker )之磁性試劑( magnetic reagent )。再使用本實驗室所研發之磁減量檢測( magnetoreduction assay ; MRA )進行特性檢測:
    (1) 豬流行性感冒病毒( Swine Influenza Virus )磁減量檢測之專一性與非專一性檢測,在本研究中可控制不同的外加交流( alternating current )磁場而抑制非專一性的結合。(2) 豬流行性感冒病毒( Swine Influenza Virus )磁減量檢測之靈敏度檢測。

    第一章 緒論................................................................................................................1 1.1 免疫檢測.................................................................................................2 1.2 豬流行性感冒病毒( Swine Influenza Virus )介紹…………………...15 第二章 磁性試劑製程方法與特性量測 2.1 磁性試劑製程方法...............................................................................17 2.2 磁性試劑物理特性量測 2.2.1 震動樣品磁化儀量測磁性流體的飽和磁化率........................21 2.2.2 雷射粒徑量測法量測磁性流體粒徑大小................................21 第三章 豬流行性感冒病毒( Swine Influenza Virus )磁減量檢測特性 3.1豬流行性感冒病毒( Swine Influenza Virus )磁減量檢測之 靈敏度量測............................................................................................24 3.2豬流行性感冒病毒( Swine Influenza Virus )磁減量檢測之 專一性與非專一性結合........................................................................29 第四章 結論..............................................................................................................33 參考文獻 致謝

    1. Horng, HE; Yang, SY; Hong, CY; Liu, CM; Tsai, PS; Yang, HC;
    Wu, CC. Biofunctionalized magnetic nanoparticles for high-sensitivity immunomagnetic detection of human C-reactive protein”, Appl. Phys. Lett. 88, 252506 (2006).
    2. http://www.miltenyibiotec.com/en/NN_27_MACS_Cell_Separation_
    Reagents.aspx
    3. Chin-Yih Hong, C.C. Wu, Y.C. Chiu, S.Y. Yang, H.E. Horng, and H.C. Yang, Magnetic susceptiblity reduction method for magnetically labeled immunoassay. Appl. Phys. Lett. 88, 212512 (2006).
    4. C. C. Wu, L. Y. Lin, L. C. Lin, H. C. Huang, Y. F. Yang, Y. B. Liu, M. C. Tsai,Y. L. Gao, W. C. Wang, S. W. Hung, S. Y. Yang, H. E. Horng, H. C. Yang, W. Y. I. Tseng, H. I. Yeh, C. F. Hsuan, T. L. Lee, and W. K. Tseng, Biofunctionalized magnetic nanoparticles for in vitro labeling and in vivo locating specific biomolecules, Appl. Phys. Lett. 92,142504(2007).
    5. http://juang.bst.ntu.edu.tw/ECX/monoclonal.htm
    6. http://www.sdix.com/
    7. http://www.accessexcellence.org/
    8. http://www.accessexcellence.org/RC/VL/GG/
    9. http://www.bio.davidson.edu/COURSES/genomics/method/ SDSPAGE/SDSPAGE. html
    10. http://en.wikipedia.org/wiki/ELISA
    11. J. Bauer, J. Chromatography, Advances in cell separation: recent developments in counterflow centrifugal elutriation and continuous flow cell separation, Biomedical Sciences and Applications, 722, 55(1999).
    12. P.J. Fisher, M.J. Springett, A.B. Dietz, P.A. Bulur, and S. Vuk-Pavlovic, Immunomagnetic separation reagents as markers in electron microscopy, J. Immunological Methods, 262, 95(2002).
    13. J. Lange, R. Kötitz, A. Haller, L. Trahms, W. Semmler and W. Weitschies,Magnetorelaxometry—a new binding specific detection method based on magnetic nanoparticles, Journal of Magnetism and Magnetic Materials, 381, 252(2002).
    14. Chin-Yih Hong, C. C. Wu, Y. C. Chiu, S. Y. Yang, H. E. Horng, and H. C. Yang, Magnetic susceptibility method for magnetically labeled immunoassay, Appl. Phys. Lett. 88,212512(2006).
    15. H. E. Horng, S. Y. Yang, Y. W. Huang, W. Q. Jiang, C.-Y. Hong, and H.C. Yang, Bio-compatible Nano-magnetic Particles for Immunoassay, IEEE Trans. Appl. Supercond., 15, 668(2005).
    16. H.E. Horng, S.Y. Yang, Chin-Yih Hong, C.M. Liu, P.S. Tsai, H.C. Yang, and C.C. Wu, “Bio-functionalized Magnetic Nano-particles for High-sensitivity Immunomagnetic Detection of Human C-reactive Protein”, Appl. Phys. Lett., 88, 252506 (2006)
    17. H.E. Horng, Chin-Yih Hong, S.Y. Yang, H.C. Yang, S.H. Liao, C.M. Liu, and C.C. Wu, “Magnetic nano-particles and their applications in immunoassay”, J. Korean Phys. Soc., 48, 999(2006)
    18. H.C. Yang, S.Y. Yang, S.H. Liao, G.L. Fang, W.H. Huang, C.H. Liu, H.E. Horng, and Chin-Yih Hong, Magnetic Relaxation Measurement in Immunoassay Using High-Tc SQUID System, J. Appl. Phys., 99, 124701 (2006)
    19. K. Enpuku, T. Minotani, T. Gima, Y. Kuroki, Y. Itoh, M. Yamashita,
    Y. Katakura, and S. Kuhara, Detection of Magnetic Nanoparticles with Superconducting Quantum Interference Device (SQUID) Magnetometer and Application to Immunoassays, Jpn. J. Appl. Phys., 38, L1102(1999)
    20. Chin-Yih Hong, C.C. Wu, Y.C. Chiu, S.Y. Yang, H.E. Horng, and H.C. Yang, Magnetic susceptiblity reduction method for magnetically labeled immunoassay. Appl. Phys. Lett. 88, 212512 (2006).
    21. Chin-Yih Hong, C.C. Wu, Y.C. Chiu, S.Y. Yang, H.E. Horng, and H.C. Yang, Magnetic susceptiblity reduction method for magnetically labeled immunoassay. Appl. Phys. Lett. 88, 212512 (2006).
    22. http://juang.bst.ntu.edu.tw/ECX/monoclonal.htm
    23. http://vettech.nvri.gov.tw/Articles/hs/1671.html
    24. http://www.nvri.gov.tw/Module/PrintFriendly/Print.aspx?
    pid=u0C75xpKiKA%3D
    25. http://www.as.nchu.edu.tw/EColi/web/dis/inf.htm
    26. Wanquan Jianga, H.C. Yanga, S.Y. Yangb, H.E. Horngb, J.C. Hungd,Y.C. Chene, Chin-Yih Hong, Preparation and properties of superparamagnetic nanoparticles with narrow size distribution and biocompatible, Journal of Magnetism and Magnetic Materials 283 (2004) 210-214.
    27. Chin-Yih Hong, W.H. Chen, C.F. Chien, S.Y. Yang, H.E. Horng, L.C. Yang, and H.C. Yang, “Wash-free immunomagnetic detection for serum through magnetic susceptibility reduction”, Appl. Phys. Lett., 90, 74105 (2007).
    28. C.C. Wu, B.F. Hong, B.H. Wu, S.Y. Yang, H.E. Horng, H.C. Yang, and W.Y. Tseng, “Animal magnetocardiography using superconducting quantum interference device gradiometers assisted with magnetic nanoparticle injection - A sensitive method for early detecting electromagnetic changes induced by hypercholesterolemia”, Appl. Phys. Lett., 90, 54111 (2007)
    29. Chin-Yih Hong, Y.H. Ke, H.E. Horng, S.Y. Yang, and H.C. Yang, Thermal activated variation in the ordered structures in magnetic fluids films, J. Magn. Magn. Mater., 289, 93(2005)
    30. H.E. Horng, C.S. Chen, K.L. Fang, S.Y. Yang, J.J. Chieh, Chin-Yih Hong, and H.C. Yang, “Tunable optical switch by using magnetic fluids”, Appl. Phys. Lett., 85, 5592(2004)

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