找到一個簡單、實用的奈米碳管探針角度修整的方法，藉由此方法可以將使用在原子力顯微術的奈米碳管探針調整成垂直於樣品，而增進空間解析。而成束的奈米碳管做成的探針才能達此形變目的，選擇適當的規則形貌樣品做掃描，則奈米碳管探針束彎折的位置、角度都可修整。並將奈米碳管探針應用在原子力顯微術，測試了奈米碳管探針對一般幾何形狀的樣品使用壽命、空間解析與穩定度。比較奈米碳管探針和一般矽製探針分別與生物樣品之間的作用力關係圖，得到奈米碳管探針在生物樣品上的表現將優於一般矽製探針的結果，故又進行了一些生物樣品的高度形貌量測、解析，進一步從事生物樣品間運作機制（去氧核醣核酸發生重組過程中的酵素作用）的研究討論。

Abstract
A simple, practical, and reliable method has been developed to bend the carbon nanotube probe of an atomic force microscope to vertically align with the sample structure. Carbon nanotubes can be plastically deformed only when they are in bundle. By scanning a patterned sample, both the bent position and angle of the attached tube probe can be adjusted. We also compare the lifetime between carbon nanotube probe and the commercial probe for normal structures. In advance, we learn the force interaction between tip and sample from force curve of an atomic force microscope. Use a carbon nanotube (CNT) probe to observe the details in some bio-molecules under atomic force microscopy (AFM) tapping mode in air. Also we study the proteins in DNA recombination.

1. H. Dai, J. H. Hafner, A. G. Rinzler, D. T. Colbert, and R. E. Smalley,
Nature (London) 384(1996) 147 .
2. G. Nagy, M. Levy, R. Scarmozzino, R. M. Osgood, Jr., H. Dai, R. E.
Smalley, C. A. Michaels, G. W. Flynn, and G. F. McLane, Appl. Phys.
Lett. 73 (1998) 529 .
3. H. Dai, N. Franklin, and J. Han, Appl. Phys. Lett. 73 (1998) 1508.
4. A. Okazaki, S. Akita, and Y. Nakayama, Physica B 323 (2002) 151.
5. H. Nishjima, S. Kamo, S. Akita, Y. Nakayama, K. I. Hohmura, S. H. Yoshimura and K. Takeyasu: Appl. Phys. Lett. 74 (1999) 4061.
6. X. Michalet: Nano Lett. 1 (2001) 341.
7. H. Nakao, H. Hayashi, T. Yoshino, S. Sugiyama, K. Otobe and T. Ohtani: Nano Lett. 2 (2002) 475.
8. A. M. Oliveira Brett and A. M. Chiorcea: Langmuir 19 (2003) 3830.
9. J. Tang, J. Li, C. Wang and C. Baia: J. Vac. Sci. & Technol. B 18 (2000) 1858.
10. T. Yoshinoa, S. Sugiyamaa, S. Hagiwaraa, D. Fukushia, M. Shichiria, H. Nakaob, J. M. Kima, T. Hirosea, H. Muramatsud and T. Ohtania: Ultramicroscopy 97 (2003) 81.
11. L. S. Shlyakhtenko, A. A. Gall, J. J. Weimer, D. D. Hawn and Y. L. Lyubchenko: Biophys. J. 77 (1999) 568.
12. L. Cai, H. Tabata and T. Kawai: Appl. Phys. Lett. 77 (2000) 3105.
13. D. Fotiadis, Y. Liang, S. Filipek, D. A. Saperstein, A. Engel and K. Palczewski: Nature 421 (2003) 127.
14. H. Yan, S. H. Park, G. Finkelstein, J. H. Reif and T. H. LaBean: Science 301 (2003) 1882.
15. H. G. Hansma: Proc. Natl. Acad. Sci. U.S.A. 96 (1999) 14678.
16. O. Medalia, J. Englander, R. Guckenberger and J. Sperling: Ultramicroscopy 90 (2002) 103.
17. J. Zheng, Z. Li, A. Wu and H. Zhou: Biophys. Chem. 104 (2003) 37.
18. C. V. Nguyen, R. M. D. Stevens, J. Barber, J. Han, M. Meyyappan, M. I. Sanchez, C. Larson and W. D. Hinsberg: Appl. Phys. Lett. 81 (2002) 901.
19. S. S. Wong, J. D. Harper, P. T. Lansbury and C. M. Lieber: J. Am. Chem. Soc. 120 (1998) 603.
20. J. H. Hafner; C.L. Cheung and C. M. Lieber: Nature 398 (1999) 761.
21. Y. C. Chang, D. C. Wang, C. S. Chang and T. T. Tsong: Appl. Phys. Lett. 82 (2003) 3541.
22. C. L. Cheung, J. H. Hafner and C. M. Lieber: Proc. Natl. Acad. Sci. U.S.A. 97 (2000) 3809.
23. A. T. Woolley, C. Guillemette, C. L. Cheung, D. E. Housman and C. M. Lieber: Nature Biotechnol. 18 (2000) 760.
24. K. I. Hohmura, Y. Itokazu, S. H. Yoshimura, G. Mizuguchi, Y.-S. Masamura, K. Takeyasu, Y. Shiomi, T. Tsurimoto, H. Nishijima, S. Akita and Y. Nakayama: J. Electron Microscopy 49 (2000) 415.
25. Y. Maeda, H. Nishijima, S. Akita, T. Matsumoto, Y. Nakayama and T. Kawai: Jpn. J. Appl. Phys. 40 (2001) 1425.
26. T. Uchihashi, N. Choi, M. Tanigawa, M. Ashno, Y. Sugawara, H. Nishjima, S. Akita, Y. Nakayama, H. Tokumoto, K. Yokoyama, S. Morita and M. Ishokawa: Jpn. J. Appl. Phys. 39 (2000) L887.
27. N. D. Jonge, Y. Lamy and M. Kaiser: Nano Lett. 3 (2003) 1621.
28.科儀新知第二十三卷第五期91.4（掃描式探針顯微鏡於生物樣本的量測與應用 吳靖宙、張憲彰）
29. V.J.Morris,A.R.Kirby,and A.P.Imperial College Press:London （1999）
30.化工資訊與商情 第三十五期 葉瑞銘、翁暢健
31. J. H. Hafner, C. L. Cheung, T. H. Oosterkamp and C. M. Lieber: J. Phys. Chem. B 105 (2001) 743.
32. Y.W.Su,C.S.Wu,C.C.Chen,and C.D.Chen,Adv.Mater.15 49（2003）
33. I.N. Senddon. Int.J.Engng Sci,3 .47（1965）
34. B.B.Akhremitchev and G.C.Walker.Langmuir,15.5630（1999）
35. F.Moreno Herrero et al Ultramicroscopy 96（2003）167-174
36. Kazuo Umemura ,et al,Biochemical and Biophysical Research Communications,281,390-395（2001）
37. Sophia I. Passy et al,Biophysics Vol.96,pp.10684-10688（1999）
38. Blat, R. U. Protacio, N. Hunter and N. Kleckner: Cell 111 (2002) 791.
39. S. I. Passy, X. Yu, Z. Li, C. M. Radding, J.-Y. Masson, S. C. West and E. H. Egelman: Proc. Natl. Acad. Sci. U.S.A. 96 (1999) 10684.