腫瘤細胞中，血管內皮細胞生長因子（vascular endothelial growth factor，VEGF）與基質金屬蛋白酶（matrix metalloproteinase，MMP）抑制劑可藉由許多不同的訊息傳遞路徑，達到具有抑制血管新生及細胞增生的效果。過去對於中草藥調節血管新生的作用機轉較少被探討。本研究使用各種不同中草藥投至人類肝癌細胞（HepG2及Huh 7），先以細胞存活率試驗初步篩選具生長抑制的藥物。實驗結果顯示，香蘇散及黃連解毒湯為複方中最具效力的待測中草藥。香蘇散能夠降低VEGF的蛋白質表現，並可藉由抑制MMP-9的RNA與蛋白質表現以抑制其蛋白質活性；初步判斷，其有效單方紫蘇葉藉由抑制VEGF表現以及MMP-9的活性以降低肝癌細胞的侵犯與血管新生作用。
黃連解毒湯可以抑制β-catenin的蛋白質表現，其有效單方黃連，除了能抑制β-catenin蛋白質表現外也會調降MMP-9的蛋白質表現與活性。
另外，單方藥物：石上柏、夏枯草、鴨膽子、苦參與黃獨均能抑制肝癌細胞生長；其中，夏枯草與黃獨亦能抑制VEGF表現，而鴨膽子與苦參是藉由抑制MMP-9及β-catenin的蛋白質表現以抑制肝癌細胞的侵犯與血管新生作用。
本實驗以蛋白質體學的方法，篩選具有調節肝癌血管新生相關因子的中草藥，達到中草藥實證效應的目標。

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謝明村（1996）中藥學概論，中國古典醫籍新解叢書（台北市）

成佳憲，行政院衛生署，中醫藥委員會95年度計畫編號： CCMP95-RD-210中藥材黃連組成物對肺癌及肝癌放射線治療動物模式之免疫調控因子基因表現

行政院衛生署，衛生統計資料（http://www.cichb.gov.tw/sta_information/sta_informations.asp）

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