Author: |
呂丹妮 Tan-Ni Lu |
---|---|
Thesis Title: |
四君子湯對人類B淋巴球分泌免疫球蛋白的調節作用 The Regulatory effect of Shy-Jiun-Tsi-Tang on Immunoglobulin |
Advisor: |
曾哲明
Tseng, Jer-Ming |
Degree: |
碩士 Master |
Department: |
生命科學系 Department of Life Science |
Academic Year: | 83 |
Language: | 中文 |
Number of pages: | 76 |
Keywords (in Chinese): | 四君子湯; 人類B淋巴球; 免疫球蛋白 |
Keywords (in English): | Shy-Jiun-Tsi-Tang; human B lymphocyte; Immunoglobuliun |
Thesis Type: | Academic thesis/ dissertation |
Reference times: | Clicks: 212 Downloads: 0 |
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四君子湯是補氣的基本處方,以黨參為主藥,佐以健脾利水的白朮、
茯苓,再配合有調和諸藥作用的炙甘草所構成。本實驗探討四君子湯
50%熱乙醇萃取液,對人類B淋巴球分泌免疫球蛋白(Ig)的調節作用。
四君子湯複方、單方,分別加入人類周邊血液與扁桃腺單核細胞
(PMNC、TMNC)培養基中四天後計算細胞總數與存活率,上清液測定Ig
分泌量。中藥對PMNC之實驗結果顯示,中藥均不影響細胞總數,四君子湯
抑制細胞存活率及IgE和IgM的分泌,茯苓抑制細胞存活率及IgE的分泌,
炙甘草抑制IgE分泌,黨參及白朮對細胞存活率及Ig分泌則無影響。
中藥對TMNC之實驗結果顯示,茯苓至抑制細胞總數,其餘中藥則
不影響細胞總數。茯苓、黨參、四君子湯,抑制細胞存活率,
白朮、炙甘草則不影響存活率。茯苓抑制TMNC產生IgM,
但不影響IgA、IgG之分泌。黨參抑制IgA、IgM,但不影響IgG。白朮抑制
IgA ,但不影響IgG、IgM,炙甘草抑制IgM,對IgA、IgG之分泌則無影響,
四君子湯則抑制IgA、IgG、IgM。中藥對PMNC、TMNC純化之B淋巴球的實驗
結果顯示,四君子湯複方、單方,都不影響細胞總數、存活率與Ig的分泌
。為瞭解中藥如何影響IgE的分泌,實驗進一步以U266IgE分泌細胞株(Cell
line)重複上述實驗,發現茯苓、黨參、四君子湯,都抑制細胞總數與存活
率,白朮、炙甘草則否,茯苓、黨參、白朮、四君子湯都抑制IgE的分泌,
炙甘草則無影響。本實驗結果證實,四君子湯確實對人類B淋巴球活性及
分泌免疫球蛋白(Ig)有調節作用,且依細胞來源不同,其影響程度亦有差
異。
Shy-Jiun-Tsi-Tang is one of the widely used Chinese herbal medi-
cine. In this study, human B lymphocytes were in vitro treated
with 50% hot ethanol extract of Shy-Jiun-Tsi-Tang and its four
major ingredients ( Dang-Shen, Bair-Jwu, Gan-Tsao and Fu-Ling ).
The concentration of IgM, IgG, IgA and IgE in the culture super-
natants were measured using an ELISA after four days of cultiva-
tion. Data obstaining from the experiments suggested that the
effects of the drugs on B-cell growth,viability and Ig secretion
were diverse in terms of the source and purity of the B lympho-
cytes. For the peripheral mononuclear cell ( PMNC ), the drugs
showed no effect on the total cell numbers. Shy-Jiun-Tsi-Tang
suppressed viability, IgM secretion and IgE secretion. Fu-Ling
suppressed viability and IgE secretion. Gan-Tsao suppressed IgE
secretion. Dang-Shen and Bair-Jwu had no effect on the viability
and Ig secretion. For the tonsil mononuclear cells ( TMNC ). Fu-
Ling suppressed the total cell numbers. Fu-Ling, Dang-Shen, Bair
-Jwu and Shy-Jiun-Tsi-Tang but Gan-Tsao significantly suppressed
on its viability. Fu-Ling suppressed IgM secretion, Dang-Shen
suppressed IgA and IgM secretion. Bair-Jwu suppressed IgA secre-
tion. Gan-Tsao suppressed IgM secretion. Shy-Jiun-Tsi-Tang sup-
pressed IgA, IgM and IgG secretiobn. For the B-lymphocytes(99.23
% in purity) from PMNC and TMNC, Shy-Jiun-Tsi-Tang and its four
major ingredients showed no effect on the total cell numbers,
viability and Ig secretion. To study the effect of the drugs on
IgE secretion further, an IgE-secreting line, U266, was used to
repeat the previous study. The data suggested that Fu-Ling, Dang
-Shen, and Shy-Jiun-Tsi-Tang significantly suppressed both the
total cell numbers and viability. Fu-Ling, Dang-Shen, Bair-Jwu
and Shy-Jiun-Tsi-Tang significantly suppressed IgE secretion by
U266 cells. This study demonstrated that Shy-Jiun-Tsi-Tang did
have regulatory effect on the growth, viability and Ig-secretion
of human B cells. The extent of the effect was mainly dependent
on the source of B lymphocytes.
Shy-Jiun-Tsi-Tang is one of the widely used Chinese herbal medi-