Basic Search / Detailed Display

Author: 李宜諺
Li, Yi-Yan
Thesis Title: 光與磁造影之超導生醫磁導儀應用於探討肝腫瘤的標靶造影
Scanning SQUID Biosusceptometery Applied to Exploring of Hepatocellular Carcinoma Targeted by Optics and Magnetic Dual-Mode Imaging.
Advisor: 謝振傑
Chieh, Jen-Jie
Degree: 碩士
Master
Department: 光電工程研究所
Graduate Institute of Electro-Optical Engineering
Thesis Publication Year: 2015
Academic Year: 103
Language: 中文
Number of pages: 29
Keywords (in Chinese): 磁性奈米粒子超導磁導儀批覆生物探針交流磁化率
Thesis Type: Academic thesis/ dissertation
Reference times: Clicks: 136Downloads: 4
Share:
School Collection Retrieve National Library Collection Retrieve Error Report
  • 現今醫學影像方面如斷層掃描、核磁共振造影、X-Ray,都具有高解析度的斷層影像,但因為維護價格高與屏蔽系統需要夠良好,所以,往往只有大型醫療機構擁有。為了增加靈敏度與專一性,批覆生物探針的奈米粒子已成為主流,利用批覆胎兒蛋白(Alpha-fetoprotein,AFP)抗體、批覆去羧機凝血脢原(Des gamma carboxy prothrombin,DCP)、批覆酯肌醇蛋白聚醣3(Glypican-3,GPC3 )抗體的磁性奈米流體,注射於癌鼠做標靶實驗,並開發出光學與磁學雙模式的超導磁導儀檢測磁性奈米粒子的交流磁化率。本實驗除了用磁導儀對注射磁流體試劑的癌鼠進行檢測,利用核磁共振造影與肝臟五葉個別量測的一致性證實了磁導儀可行性。因此,未來病患可注射批覆抗體的磁性奈米粒子試劑後進行活體篩檢,如果有腫瘤症狀,需要進一步確認再進行高額的其他檢查,減少民眾負擔。

    目錄 摘要..........................I 目錄.......................... II 圖表目錄.......................... IV 第一章 緒論..........................1 第二章 研究理論.......................... 3 2.1 磁導儀磁訊號量測理論..........................3 第三章 磁性奈米粒子與標靶試劑檢測方法.....................9 3.1 磁性奈米流體備製..........................9 圖3.1批覆抗體磁性流體備置流程........................10 3.2激發線圈與感應線圈設計與製作..................10 圖3.2激發線圈側視與底視..........................11 3.3雙模式磁導儀系統設計與製作..........................11 第四章 實驗結果..........................13 4.1 雙模式探頭性能測試..........................13 4.1.1假體測試..........................13 4.1.2磁性奈米粒子流體不同濃度相同深度量測..........13 4.2動物研究測試..........................16 4.2.1 實驗規劃..........................16 4.2.2影像疊合與間歇性量測測試.................16 4.2.3背部腫瘤..........................17 4.2.4肝臟腫瘤..........................18 第五章 結論..........................24 參考文獻..........................25 致謝..........................28 圖表目錄 圖 2.1生物磁場強度與超導量子干涉儀的靈敏度........4 圖2.2磁量耦合示意圖..........................6 圖2.3 rf SQUID與共振電路形成互感的等效電路........7 圖2.4為SQUID工作電路..........................8 圖3.1批覆抗體磁性流體備置流程..........................10 圖3.2激發線圈側視與底視..........................11 圖3.3光學與磁學雙模式造影系統..........................12 圖4.1假體測試磁影像與強度比較..........................13 圖4.3 不同MF濃度與不同探頭深度,光學與磁學影像疊合.....15 圖4.4不同探頭深度不同與MF濃度之強度總合比較............15 圖4.5標準曲線圖..........................15 圖4.6間歇掃描檢查[26]、連續靜態檢查........17 圖4.7背部腫瘤老鼠注射試劑前後強度影像.......18 圖4.8掃描範圍與不同時間量測強度比較........20 圖4.9核磁共振造影與訊號比較................21 圖4.10手術引導肝腫瘤之磁導儀量測............22 圖4.11不同試劑不同時間點量測強度比較.........23

    參考文獻
    [1]行政院衛生福利部104年國人死因統計結果
    [2]Hong CY,Wu CC,Yang SY,et al.Magnetic susceptibility reduction method for magnrtically labeled immunoassay. Appl Phys Lett.;88:212512-1(2006).
    [3] Huang KW,Yang SY,Hong YW,et al.Feasibility studies for
    Assaying alpha-fetoprotein using antibody-activated magnetic nanoparticles.Int J Nanomed.;7:1991(2012)
    [4]Rosensweig RE.Heating magnetic fluid with alternating magnetic field.J Magn Magn Mater.;252:370(2002)
    [5]Tseng HY,Lee GB,Lee CY,et al.Localised heating of tumors
    Utilizing injectable magnetic nanoparticles for hyperthermia cancer therapy.IET Nanobiotechnol.;3(2):46(2009)
    [6]K -W Huang, H -H Chen, H -C Yang, H -E Horng, S -H Liao, J -J Chieh and S Y Yang. Use of a high-Tc SQUID-based nuclear magnetic resonance spectrometer in magnetically unshielded environments to discriminate tumors in rats, by characterizing the longitudinal relaxation rate. Journal of Instrumentation.; 7:P06005(2012).
    [7]Moritz FK,Umar M,Raymond SK,et al.A multimodal nanoparticle
    for preoperative magnetic delineation resonance imaging and intraoperative optical brain tumor.Cancer Res.;63:8122(2003)
    [8]Watkin KL,McDonald MA.Multi-modal contrast agents:a first step.Academic Radiology 9:S285(2002)
    [9]Chieh JJ,Hong CY,.Non-invasive and High-sensitivity scanning detection of magnetic nanoparticles in animals using high-Tc scanning superconductingquantum-interference-device biosusceptometry.Rev Sci Instrum.82:084301-1(2011)
    [10]Martin Nikolo.A guide to alternating current susceptibility measurements and alternating current susceptometer design.American Association oh Physics 57-65
    [11]Chieh JJ,Tseng WK,Horng HE,et al.In-vivo and real-time measurements of magnetic-nanoparticles distribution in animals by scanning SQUID biosusceptometry for biomedicine study.IEEE IEEE Trans Biomed Eng.;58(10):2719(2011)
    [12]Tseng WK,Chieh JJ,Yang SY,et al.In-vivo and fast examination of iron concertration of magnetic nanoparticles in an animal torso via sanning SQUID Biosusceptometry. IEEE Trans Appl Supercond.;21(3):2250-2253(2011)
    [13]Tadayuki Kondol and Hideo Itozaki.Normal conducting transfer coil for SQUID. NDE SUPERCONDUCTOR SCIENCE AND TECHNOLOGY,17,459(2004)
    [14]Y.S Greenberg.Application of superconducting quantum interference devices to nuclear magnetic resonance.Rev.Mod.Phys.,vol.70:175(2002)
    [15]R.McDermott,A.H Trabesinger,M.Muck,E.L.Haln,A.pines,and J. Clark,Liquid-state NMR and scalar couplings in microtesla magenetic fields,Science,vol.295:2247(2002)
    [16]Y.Zhang,L.Qiu,H.Krause,S.Hartiwig,M.Burghoff,and L.Trahms,Liquid state nuclear magnetic resonance at low fields using a nitrogen cooled superconducting quantum interference device,Appl.Phys.Lett.,90:182503(2007)
    [17]K.Schlenga,R.McDermott,J.Clark,R.E.de Souza,A.Wong-Foy,and A.Pines,Low-field magnetic resonance imaging with a high-Tc dc superconducting quantum interference device, Appl.Phys.Lett.,75:3695(1999)
    [18]M.Burghoff,S.Hartwig,L.Trahms,and J.Bernarding,Nuclear magntic resonance in the nanoTesla range, Appl.Phys.Lett.,87:054103,(2005)
    [19]L.Qiu,Y.Zhang,H.J Krause,A.H Brainski,M.Burghoff,and L.Trahms,Nuclear magnetic resonance in the earth’s magnetic field using a nitrogen-cooled superconducting quantum interference device.APPLIED PHYSICS LETTERS,91:072505(2007)
    [21]Horng HE,Yang SY,Huang YW,Jiang WQ,Hong CY,et al.Nanomagnetic Perticles for SQUID-based Magnetically Labeled Immunoassay,IEEE Trans Appl Supercond.15:668(2005)
    [22]Yang SY,Jian ZF,Horng HE,Hong CY,Yang HC,et al.Dualimmobilization and magnetic manipulation of magnetic nanoparticles.J MagnMagn Mater.320:2688(2008)
    [23]Chaur-Shine Wang,Chih-Lin Lin,His-Chang Lee,Kuan-Yang Chen,Ming-Feng Chiang,Huan-Sheng Chen,Tsung-Jung Lin,Li-Yang Liao.Usefulness of serum des-y-carboxy prothrombin in detection of hepatocellular carcinoma,World J Gastroenterol.11:6115-6119(39)(2005)
    [24]Jukubovic BD,Jothy S.Glypican-3:form the mutations of Simpson-Golabi-Behmel genetic syndrome to a tumor marker for hepatocellular carcinoma,Exp Mol Pathol.82:184-9(2)(2007)

    [25]羅錦河(2014)肝癌之症狀與篩檢,高雄榮民總醫院衛教期刊,取自http://www.vghks.gov.tw/gi/htm/本科衛教/肝癌之症狀與篩檢.htm
    [26]J.J Chieh and C.Y Hong.Non-invasive and High-sensitivity scanning detection of magnetic nanoparticles in animals using high-Tc superconducting-quantum-interference-device

    下載圖示
    QR CODE